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The IDA-LIKE peptides IDL6 and IDL7 are negative modulators of stress responses in Arabidopsis thaliana

Small signalling peptides have emerged as important cell to cell messengers in plant development and stress responses. However, only a few of the predicted peptides have been functionally characterized. Here, we present functional characterization of two members of the IDA-LIKE (IDL) peptide family...

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Autores principales: Vie, Ane Kjersti, Najafi, Javad, Winge, Per, Cattan, Ester, Wrzaczek, Michael, Kangasjärvi, Jaakko, Miller, Gad, Brembu, Tore, Bones, Atle M
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5853212/
https://www.ncbi.nlm.nih.gov/pubmed/28586470
http://dx.doi.org/10.1093/jxb/erx168
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author Vie, Ane Kjersti
Najafi, Javad
Winge, Per
Cattan, Ester
Wrzaczek, Michael
Kangasjärvi, Jaakko
Miller, Gad
Brembu, Tore
Bones, Atle M
author_facet Vie, Ane Kjersti
Najafi, Javad
Winge, Per
Cattan, Ester
Wrzaczek, Michael
Kangasjärvi, Jaakko
Miller, Gad
Brembu, Tore
Bones, Atle M
author_sort Vie, Ane Kjersti
collection PubMed
description Small signalling peptides have emerged as important cell to cell messengers in plant development and stress responses. However, only a few of the predicted peptides have been functionally characterized. Here, we present functional characterization of two members of the IDA-LIKE (IDL) peptide family in Arabidopsis thaliana, IDL6 and IDL7. Localization studies suggest that the peptides require a signal peptide and C-terminal processing to be correctly transported out of the cell. Both IDL6 and IDL7 appear to be unstable transcripts under post-transcriptional regulation. Treatment of plants with synthetic IDL6 and IDL7 peptides resulted in down-regulation of a broad range of stress-responsive genes, including early stress-responsive transcripts, dominated by a large group of ZINC FINGER PROTEIN (ZFP) genes, WRKY genes, and genes encoding calcium-dependent proteins. IDL7 expression was rapidly induced by hydrogen peroxide, and idl7 and idl6 idl7 double mutants displayed reduced cell death upon exposure to extracellular reactive oxygen species (ROS). Co-treatment of the bacterial elicitor flg22 with IDL7 peptide attenuated the rapid ROS burst induced by treatment with flg22 alone. Taken together, our results suggest that IDL7, and possibly IDL6, act as negative modulators of stress-induced ROS signalling in Arabidopsis.
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spelling pubmed-58532122018-07-25 The IDA-LIKE peptides IDL6 and IDL7 are negative modulators of stress responses in Arabidopsis thaliana Vie, Ane Kjersti Najafi, Javad Winge, Per Cattan, Ester Wrzaczek, Michael Kangasjärvi, Jaakko Miller, Gad Brembu, Tore Bones, Atle M J Exp Bot Research Papers Small signalling peptides have emerged as important cell to cell messengers in plant development and stress responses. However, only a few of the predicted peptides have been functionally characterized. Here, we present functional characterization of two members of the IDA-LIKE (IDL) peptide family in Arabidopsis thaliana, IDL6 and IDL7. Localization studies suggest that the peptides require a signal peptide and C-terminal processing to be correctly transported out of the cell. Both IDL6 and IDL7 appear to be unstable transcripts under post-transcriptional regulation. Treatment of plants with synthetic IDL6 and IDL7 peptides resulted in down-regulation of a broad range of stress-responsive genes, including early stress-responsive transcripts, dominated by a large group of ZINC FINGER PROTEIN (ZFP) genes, WRKY genes, and genes encoding calcium-dependent proteins. IDL7 expression was rapidly induced by hydrogen peroxide, and idl7 and idl6 idl7 double mutants displayed reduced cell death upon exposure to extracellular reactive oxygen species (ROS). Co-treatment of the bacterial elicitor flg22 with IDL7 peptide attenuated the rapid ROS burst induced by treatment with flg22 alone. Taken together, our results suggest that IDL7, and possibly IDL6, act as negative modulators of stress-induced ROS signalling in Arabidopsis. Oxford University Press 2017-06-15 2017-06-06 /pmc/articles/PMC5853212/ /pubmed/28586470 http://dx.doi.org/10.1093/jxb/erx168 Text en © The Author 2017. Published by Oxford University Press on behalf of the Society for Experimental Biology. http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Papers
Vie, Ane Kjersti
Najafi, Javad
Winge, Per
Cattan, Ester
Wrzaczek, Michael
Kangasjärvi, Jaakko
Miller, Gad
Brembu, Tore
Bones, Atle M
The IDA-LIKE peptides IDL6 and IDL7 are negative modulators of stress responses in Arabidopsis thaliana
title The IDA-LIKE peptides IDL6 and IDL7 are negative modulators of stress responses in Arabidopsis thaliana
title_full The IDA-LIKE peptides IDL6 and IDL7 are negative modulators of stress responses in Arabidopsis thaliana
title_fullStr The IDA-LIKE peptides IDL6 and IDL7 are negative modulators of stress responses in Arabidopsis thaliana
title_full_unstemmed The IDA-LIKE peptides IDL6 and IDL7 are negative modulators of stress responses in Arabidopsis thaliana
title_short The IDA-LIKE peptides IDL6 and IDL7 are negative modulators of stress responses in Arabidopsis thaliana
title_sort ida-like peptides idl6 and idl7 are negative modulators of stress responses in arabidopsis thaliana
topic Research Papers
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5853212/
https://www.ncbi.nlm.nih.gov/pubmed/28586470
http://dx.doi.org/10.1093/jxb/erx168
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