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Live Attenuated Pertussis Vaccine BPZE1 Protects Baboons Against Bordetella pertussis Disease and Infection

Evidence suggests that the resurgence of pertussis in many industrialized countries may result from the failure of current vaccines to prevent nasopharyngeal colonization by Bordetella pertussis, the principal causative agent of whooping cough. Here, we used a baboon model to test the protective pot...

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Autores principales: Locht, Camille, Papin, James F., Lecher, Sophie, Debrie, Anne-Sophie, Thalen, Marcel, Solovay, Ken, Rubin, Keith, Mielcarek, Nathalie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5853371/
https://www.ncbi.nlm.nih.gov/pubmed/28535276
http://dx.doi.org/10.1093/infdis/jix254
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author Locht, Camille
Papin, James F.
Lecher, Sophie
Debrie, Anne-Sophie
Thalen, Marcel
Solovay, Ken
Rubin, Keith
Mielcarek, Nathalie
author_facet Locht, Camille
Papin, James F.
Lecher, Sophie
Debrie, Anne-Sophie
Thalen, Marcel
Solovay, Ken
Rubin, Keith
Mielcarek, Nathalie
author_sort Locht, Camille
collection PubMed
description Evidence suggests that the resurgence of pertussis in many industrialized countries may result from the failure of current vaccines to prevent nasopharyngeal colonization by Bordetella pertussis, the principal causative agent of whooping cough. Here, we used a baboon model to test the protective potential of the novel, live attenuated pertussis vaccine candidate BPZE1. A single intranasal/intratracheal inoculation of juvenile baboons with BPZE1 resulted in transient nasopharyngeal colonization and induction of immunoglobulin G and immunoglobulin A to all antigens tested, while causing no adverse symptoms or leukocytosis. When BPZE1-vaccinated baboons were challenged with a high dose of a highly virulent B. pertussis isolate, they were fully protected against disease, whereas naive baboons developed illness (with 1 death) and leukocytosis. Total postchallenge nasopharyngeal virulent bacterial burden of vaccinated animals was substantially reduced (0.002%) compared to naive controls, providing promising evidence in nonhuman primates that BPZE1 protects against both pertussis disease and B. pertussis infection.
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spelling pubmed-58533712018-03-23 Live Attenuated Pertussis Vaccine BPZE1 Protects Baboons Against Bordetella pertussis Disease and Infection Locht, Camille Papin, James F. Lecher, Sophie Debrie, Anne-Sophie Thalen, Marcel Solovay, Ken Rubin, Keith Mielcarek, Nathalie J Infect Dis Major Article Evidence suggests that the resurgence of pertussis in many industrialized countries may result from the failure of current vaccines to prevent nasopharyngeal colonization by Bordetella pertussis, the principal causative agent of whooping cough. Here, we used a baboon model to test the protective potential of the novel, live attenuated pertussis vaccine candidate BPZE1. A single intranasal/intratracheal inoculation of juvenile baboons with BPZE1 resulted in transient nasopharyngeal colonization and induction of immunoglobulin G and immunoglobulin A to all antigens tested, while causing no adverse symptoms or leukocytosis. When BPZE1-vaccinated baboons were challenged with a high dose of a highly virulent B. pertussis isolate, they were fully protected against disease, whereas naive baboons developed illness (with 1 death) and leukocytosis. Total postchallenge nasopharyngeal virulent bacterial burden of vaccinated animals was substantially reduced (0.002%) compared to naive controls, providing promising evidence in nonhuman primates that BPZE1 protects against both pertussis disease and B. pertussis infection. Oxford University Press 2017-07-01 2017-05-23 /pmc/articles/PMC5853371/ /pubmed/28535276 http://dx.doi.org/10.1093/infdis/jix254 Text en © The Author 2017. Published by Oxford University Press for the Infectious Diseases Society of America. http://creativecommons.org/licenses/by-nc-nd/4.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs licence (http://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial reproduction and distribution of the work, in any medium, provided the original work is not altered or transformed in any way, and that the work is properly cited. For commercial re-use, please contact journals.permissions@oup.com
spellingShingle Major Article
Locht, Camille
Papin, James F.
Lecher, Sophie
Debrie, Anne-Sophie
Thalen, Marcel
Solovay, Ken
Rubin, Keith
Mielcarek, Nathalie
Live Attenuated Pertussis Vaccine BPZE1 Protects Baboons Against Bordetella pertussis Disease and Infection
title Live Attenuated Pertussis Vaccine BPZE1 Protects Baboons Against Bordetella pertussis Disease and Infection
title_full Live Attenuated Pertussis Vaccine BPZE1 Protects Baboons Against Bordetella pertussis Disease and Infection
title_fullStr Live Attenuated Pertussis Vaccine BPZE1 Protects Baboons Against Bordetella pertussis Disease and Infection
title_full_unstemmed Live Attenuated Pertussis Vaccine BPZE1 Protects Baboons Against Bordetella pertussis Disease and Infection
title_short Live Attenuated Pertussis Vaccine BPZE1 Protects Baboons Against Bordetella pertussis Disease and Infection
title_sort live attenuated pertussis vaccine bpze1 protects baboons against bordetella pertussis disease and infection
topic Major Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5853371/
https://www.ncbi.nlm.nih.gov/pubmed/28535276
http://dx.doi.org/10.1093/infdis/jix254
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