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The role of Rubisco kinetics and pyrenoid morphology in shaping the CCM of haptophyte microalgae

The haptophyte algae are a cosmopolitan group of primary producers that contribute significantly to the marine carbon cycle and play a major role in paleo-climate studies. Despite their global importance, little is known about carbon assimilation in haptophytes, in particular the kinetics of their F...

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Autores principales: Heureux, Ana M C, Young, Jodi N, Whitney, Spencer M, Eason-Hubbard, Maeve R, Lee, Renee B Y, Sharwood, Robert E, Rickaby, Rosalind E M
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5853415/
https://www.ncbi.nlm.nih.gov/pubmed/28582571
http://dx.doi.org/10.1093/jxb/erx179
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author Heureux, Ana M C
Young, Jodi N
Whitney, Spencer M
Eason-Hubbard, Maeve R
Lee, Renee B Y
Sharwood, Robert E
Rickaby, Rosalind E M
author_facet Heureux, Ana M C
Young, Jodi N
Whitney, Spencer M
Eason-Hubbard, Maeve R
Lee, Renee B Y
Sharwood, Robert E
Rickaby, Rosalind E M
author_sort Heureux, Ana M C
collection PubMed
description The haptophyte algae are a cosmopolitan group of primary producers that contribute significantly to the marine carbon cycle and play a major role in paleo-climate studies. Despite their global importance, little is known about carbon assimilation in haptophytes, in particular the kinetics of their Form 1D CO(2)-fixing enzyme, Rubisco. Here we examine Rubisco properties of three haptophytes with a range of pyrenoid morphologies (Pleurochrysis carterae, Tisochrysis lutea, and Pavlova lutheri) and the diatom Phaeodactylum tricornutum that exhibit contrasting sensitivities to the trade-offs between substrate affinity (K(m)) and turnover rate (k(cat)) for both CO(2) and O(2). The pyrenoid-containing T. lutea and P. carterae showed lower Rubisco content and carboxylation properties (K(C) and k(C)(cat)) comparable with those of Form 1D-containing non-green algae. In contrast, the pyrenoid-lacking P. lutheri produced Rubisco in 3-fold higher amounts, and displayed a Form 1B Rubisco k(C)(cat)–K(C) relationship and increased CO(2)/O(2) specificity that, when modeled in the context of a C(3) leaf, supported equivalent rates of photosynthesis to higher plant Rubisco. Correlation between the differing Rubisco properties and the occurrence and localization of pyrenoids with differing intracellular CO(2):O(2) microenvironments has probably influenced the divergent evolution of Form 1B and 1D Rubisco kinetics.
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spelling pubmed-58534152018-07-25 The role of Rubisco kinetics and pyrenoid morphology in shaping the CCM of haptophyte microalgae Heureux, Ana M C Young, Jodi N Whitney, Spencer M Eason-Hubbard, Maeve R Lee, Renee B Y Sharwood, Robert E Rickaby, Rosalind E M J Exp Bot Research Papers The haptophyte algae are a cosmopolitan group of primary producers that contribute significantly to the marine carbon cycle and play a major role in paleo-climate studies. Despite their global importance, little is known about carbon assimilation in haptophytes, in particular the kinetics of their Form 1D CO(2)-fixing enzyme, Rubisco. Here we examine Rubisco properties of three haptophytes with a range of pyrenoid morphologies (Pleurochrysis carterae, Tisochrysis lutea, and Pavlova lutheri) and the diatom Phaeodactylum tricornutum that exhibit contrasting sensitivities to the trade-offs between substrate affinity (K(m)) and turnover rate (k(cat)) for both CO(2) and O(2). The pyrenoid-containing T. lutea and P. carterae showed lower Rubisco content and carboxylation properties (K(C) and k(C)(cat)) comparable with those of Form 1D-containing non-green algae. In contrast, the pyrenoid-lacking P. lutheri produced Rubisco in 3-fold higher amounts, and displayed a Form 1B Rubisco k(C)(cat)–K(C) relationship and increased CO(2)/O(2) specificity that, when modeled in the context of a C(3) leaf, supported equivalent rates of photosynthesis to higher plant Rubisco. Correlation between the differing Rubisco properties and the occurrence and localization of pyrenoids with differing intracellular CO(2):O(2) microenvironments has probably influenced the divergent evolution of Form 1B and 1D Rubisco kinetics. Oxford University Press 2017-06-22 2017-06-03 /pmc/articles/PMC5853415/ /pubmed/28582571 http://dx.doi.org/10.1093/jxb/erx179 Text en © The Author 2017. Published by Oxford University Press on behalf of the Society for Experimental Biology. http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Papers
Heureux, Ana M C
Young, Jodi N
Whitney, Spencer M
Eason-Hubbard, Maeve R
Lee, Renee B Y
Sharwood, Robert E
Rickaby, Rosalind E M
The role of Rubisco kinetics and pyrenoid morphology in shaping the CCM of haptophyte microalgae
title The role of Rubisco kinetics and pyrenoid morphology in shaping the CCM of haptophyte microalgae
title_full The role of Rubisco kinetics and pyrenoid morphology in shaping the CCM of haptophyte microalgae
title_fullStr The role of Rubisco kinetics and pyrenoid morphology in shaping the CCM of haptophyte microalgae
title_full_unstemmed The role of Rubisco kinetics and pyrenoid morphology in shaping the CCM of haptophyte microalgae
title_short The role of Rubisco kinetics and pyrenoid morphology in shaping the CCM of haptophyte microalgae
title_sort role of rubisco kinetics and pyrenoid morphology in shaping the ccm of haptophyte microalgae
topic Research Papers
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5853415/
https://www.ncbi.nlm.nih.gov/pubmed/28582571
http://dx.doi.org/10.1093/jxb/erx179
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