Cargando…
Haustorium initiation in the obligate parasitic plant Phelipanche ramosa involves a host-exudated cytokinin signal
The heterotrophic lifestyle of parasitic plants relies on the development of the haustorium, a specific infectious organ required for attachment to host roots. While haustorium development is initiated upon chemodetection of host-derived molecules in hemiparasitic plants, the induction of haustorium...
Autores principales: | , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2017
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5853424/ https://www.ncbi.nlm.nih.gov/pubmed/29069455 http://dx.doi.org/10.1093/jxb/erx359 |
_version_ | 1783306753914437632 |
---|---|
author | Goyet, Vincent Billard, Estelle Pouvreau, Jean-Bernard Lechat, Marc-Marie Pelletier, Sandra Bahut, Muriel Monteau, Fabrice Spíchal, Lukáš Delavault, Philippe Montiel, Grégory Simier, Philippe |
author_facet | Goyet, Vincent Billard, Estelle Pouvreau, Jean-Bernard Lechat, Marc-Marie Pelletier, Sandra Bahut, Muriel Monteau, Fabrice Spíchal, Lukáš Delavault, Philippe Montiel, Grégory Simier, Philippe |
author_sort | Goyet, Vincent |
collection | PubMed |
description | The heterotrophic lifestyle of parasitic plants relies on the development of the haustorium, a specific infectious organ required for attachment to host roots. While haustorium development is initiated upon chemodetection of host-derived molecules in hemiparasitic plants, the induction of haustorium formation remains largely unknown in holoparasitic species such as Phelipanche ramosa. This work demonstrates that the root exudates of the host plant Brassica napus contain allelochemicals displaying haustorium-inducing activity on P. ramosa germinating seeds, which increases the parasite aggressiveness. A de novo assembled transcriptome and microarray approach with P. ramosa during early haustorium formation upon treatment with B. napus root exudates allowed the identification of differentially expressed genes involved in hormone signaling. Bioassays using exogenous cytokinins and the specific cytokinin receptor inhibitor PI-55 showed that cytokinins induced haustorium formation and increased parasite aggressiveness. Root exudates triggered the expression of cytokinin-responsive genes during early haustorium development in germinated seeds, and bio-guided UPLC-ESI(+)-/MS/MS analysis showed that these exudates contain a cytokinin with dihydrozeatin characteristics. These results suggest that cytokinins constitutively exudated from host roots play a major role in haustorium formation and aggressiveness in P. ramosa. |
format | Online Article Text |
id | pubmed-5853424 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-58534242018-07-25 Haustorium initiation in the obligate parasitic plant Phelipanche ramosa involves a host-exudated cytokinin signal Goyet, Vincent Billard, Estelle Pouvreau, Jean-Bernard Lechat, Marc-Marie Pelletier, Sandra Bahut, Muriel Monteau, Fabrice Spíchal, Lukáš Delavault, Philippe Montiel, Grégory Simier, Philippe J Exp Bot Research Papers The heterotrophic lifestyle of parasitic plants relies on the development of the haustorium, a specific infectious organ required for attachment to host roots. While haustorium development is initiated upon chemodetection of host-derived molecules in hemiparasitic plants, the induction of haustorium formation remains largely unknown in holoparasitic species such as Phelipanche ramosa. This work demonstrates that the root exudates of the host plant Brassica napus contain allelochemicals displaying haustorium-inducing activity on P. ramosa germinating seeds, which increases the parasite aggressiveness. A de novo assembled transcriptome and microarray approach with P. ramosa during early haustorium formation upon treatment with B. napus root exudates allowed the identification of differentially expressed genes involved in hormone signaling. Bioassays using exogenous cytokinins and the specific cytokinin receptor inhibitor PI-55 showed that cytokinins induced haustorium formation and increased parasite aggressiveness. Root exudates triggered the expression of cytokinin-responsive genes during early haustorium development in germinated seeds, and bio-guided UPLC-ESI(+)-/MS/MS analysis showed that these exudates contain a cytokinin with dihydrozeatin characteristics. These results suggest that cytokinins constitutively exudated from host roots play a major role in haustorium formation and aggressiveness in P. ramosa. Oxford University Press 2017-09-01 2017-10-21 /pmc/articles/PMC5853424/ /pubmed/29069455 http://dx.doi.org/10.1093/jxb/erx359 Text en © The Author 2017. Published by Oxford University Press on behalf of the Society for Experimental Biology. http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Papers Goyet, Vincent Billard, Estelle Pouvreau, Jean-Bernard Lechat, Marc-Marie Pelletier, Sandra Bahut, Muriel Monteau, Fabrice Spíchal, Lukáš Delavault, Philippe Montiel, Grégory Simier, Philippe Haustorium initiation in the obligate parasitic plant Phelipanche ramosa involves a host-exudated cytokinin signal |
title | Haustorium initiation in the obligate parasitic plant Phelipanche ramosa involves a host-exudated cytokinin signal |
title_full | Haustorium initiation in the obligate parasitic plant Phelipanche ramosa involves a host-exudated cytokinin signal |
title_fullStr | Haustorium initiation in the obligate parasitic plant Phelipanche ramosa involves a host-exudated cytokinin signal |
title_full_unstemmed | Haustorium initiation in the obligate parasitic plant Phelipanche ramosa involves a host-exudated cytokinin signal |
title_short | Haustorium initiation in the obligate parasitic plant Phelipanche ramosa involves a host-exudated cytokinin signal |
title_sort | haustorium initiation in the obligate parasitic plant phelipanche ramosa involves a host-exudated cytokinin signal |
topic | Research Papers |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5853424/ https://www.ncbi.nlm.nih.gov/pubmed/29069455 http://dx.doi.org/10.1093/jxb/erx359 |
work_keys_str_mv | AT goyetvincent haustoriuminitiationintheobligateparasiticplantphelipancheramosainvolvesahostexudatedcytokininsignal AT billardestelle haustoriuminitiationintheobligateparasiticplantphelipancheramosainvolvesahostexudatedcytokininsignal AT pouvreaujeanbernard haustoriuminitiationintheobligateparasiticplantphelipancheramosainvolvesahostexudatedcytokininsignal AT lechatmarcmarie haustoriuminitiationintheobligateparasiticplantphelipancheramosainvolvesahostexudatedcytokininsignal AT pelletiersandra haustoriuminitiationintheobligateparasiticplantphelipancheramosainvolvesahostexudatedcytokininsignal AT bahutmuriel haustoriuminitiationintheobligateparasiticplantphelipancheramosainvolvesahostexudatedcytokininsignal AT monteaufabrice haustoriuminitiationintheobligateparasiticplantphelipancheramosainvolvesahostexudatedcytokininsignal AT spichallukas haustoriuminitiationintheobligateparasiticplantphelipancheramosainvolvesahostexudatedcytokininsignal AT delavaultphilippe haustoriuminitiationintheobligateparasiticplantphelipancheramosainvolvesahostexudatedcytokininsignal AT montielgregory haustoriuminitiationintheobligateparasiticplantphelipancheramosainvolvesahostexudatedcytokininsignal AT simierphilippe haustoriuminitiationintheobligateparasiticplantphelipancheramosainvolvesahostexudatedcytokininsignal |