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Delaying chloroplast turnover increases water-deficit stress tolerance through the enhancement of nitrogen assimilation in rice
Abiotic stress-induced senescence in crops is a process particularly affecting the photosynthetic apparatus, decreasing photosynthetic activity and inducing chloroplast degradation. A pathway for stress-induced chloroplast degradation that involves the CHLOROPLAST VESICULATION (CV) gene was characte...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5853860/ https://www.ncbi.nlm.nih.gov/pubmed/28992306 http://dx.doi.org/10.1093/jxb/erx247 |
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author | Sade, Nir Umnajkitikorn, Kamolchanok Rubio Wilhelmi, Maria del Mar Wright, Matthew Wang, Songhu Blumwald, Eduardo |
author_facet | Sade, Nir Umnajkitikorn, Kamolchanok Rubio Wilhelmi, Maria del Mar Wright, Matthew Wang, Songhu Blumwald, Eduardo |
author_sort | Sade, Nir |
collection | PubMed |
description | Abiotic stress-induced senescence in crops is a process particularly affecting the photosynthetic apparatus, decreasing photosynthetic activity and inducing chloroplast degradation. A pathway for stress-induced chloroplast degradation that involves the CHLOROPLAST VESICULATION (CV) gene was characterized in rice (Oryza sativa) plants. OsCV expression was up-regulated with the age of the plants and when plants were exposed to water-deficit conditions. The down-regulation of OsCV expression contributed to the maintenance of the chloroplast integrity under stress. OsCV-silenced plants displayed enhanced source fitness (i.e. carbon and nitrogen assimilation) and photorespiration, leading to water-deficit stress tolerance. Co-immunoprecipitation, intracellular co-localization, and bimolecular fluorescence demonstrated the in vivo interaction between OsCV and chloroplastic glutamine synthetase (OsGS2), affecting source–sink relationships of the plants under stress. Our results would indicate that the OsCV-mediated chloroplast degradation pathway is involved in the regulation of nitrogen assimilation during stress-induced plant senescence. |
format | Online Article Text |
id | pubmed-5853860 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-58538602018-07-12 Delaying chloroplast turnover increases water-deficit stress tolerance through the enhancement of nitrogen assimilation in rice Sade, Nir Umnajkitikorn, Kamolchanok Rubio Wilhelmi, Maria del Mar Wright, Matthew Wang, Songhu Blumwald, Eduardo J Exp Bot Research Papers Abiotic stress-induced senescence in crops is a process particularly affecting the photosynthetic apparatus, decreasing photosynthetic activity and inducing chloroplast degradation. A pathway for stress-induced chloroplast degradation that involves the CHLOROPLAST VESICULATION (CV) gene was characterized in rice (Oryza sativa) plants. OsCV expression was up-regulated with the age of the plants and when plants were exposed to water-deficit conditions. The down-regulation of OsCV expression contributed to the maintenance of the chloroplast integrity under stress. OsCV-silenced plants displayed enhanced source fitness (i.e. carbon and nitrogen assimilation) and photorespiration, leading to water-deficit stress tolerance. Co-immunoprecipitation, intracellular co-localization, and bimolecular fluorescence demonstrated the in vivo interaction between OsCV and chloroplastic glutamine synthetase (OsGS2), affecting source–sink relationships of the plants under stress. Our results would indicate that the OsCV-mediated chloroplast degradation pathway is involved in the regulation of nitrogen assimilation during stress-induced plant senescence. Oxford University Press 2018-02-06 2017-07-27 /pmc/articles/PMC5853860/ /pubmed/28992306 http://dx.doi.org/10.1093/jxb/erx247 Text en © The Author(s) 2017. Published by Oxford University Press on behalf of the Society for Experimental Biology. http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Papers Sade, Nir Umnajkitikorn, Kamolchanok Rubio Wilhelmi, Maria del Mar Wright, Matthew Wang, Songhu Blumwald, Eduardo Delaying chloroplast turnover increases water-deficit stress tolerance through the enhancement of nitrogen assimilation in rice |
title | Delaying chloroplast turnover increases water-deficit stress tolerance through the enhancement of nitrogen assimilation in rice |
title_full | Delaying chloroplast turnover increases water-deficit stress tolerance through the enhancement of nitrogen assimilation in rice |
title_fullStr | Delaying chloroplast turnover increases water-deficit stress tolerance through the enhancement of nitrogen assimilation in rice |
title_full_unstemmed | Delaying chloroplast turnover increases water-deficit stress tolerance through the enhancement of nitrogen assimilation in rice |
title_short | Delaying chloroplast turnover increases water-deficit stress tolerance through the enhancement of nitrogen assimilation in rice |
title_sort | delaying chloroplast turnover increases water-deficit stress tolerance through the enhancement of nitrogen assimilation in rice |
topic | Research Papers |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5853860/ https://www.ncbi.nlm.nih.gov/pubmed/28992306 http://dx.doi.org/10.1093/jxb/erx247 |
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