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Subcellular localization and interactions among rubber particle proteins from Hevea brasiliensis

Natural rubber (polyisoprene) from the rubber tree Hevea brasiliensis is synthesized by specialized cells called laticifers. It is not clear how rubber particles arise, although one hypothesis is that they derive from the endoplasmic reticulum (ER) membrane. Here we cloned the genes encoding four ke...

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Autores principales: Brown, Daniel, Feeney, Mistianne, Ahmadi, Mathin, Lonoce, Chiara, Sajari, Roslinda, Di Cola, Alessandra, Frigerio, Lorenzo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5853894/
https://www.ncbi.nlm.nih.gov/pubmed/29036360
http://dx.doi.org/10.1093/jxb/erx331
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author Brown, Daniel
Feeney, Mistianne
Ahmadi, Mathin
Lonoce, Chiara
Sajari, Roslinda
Di Cola, Alessandra
Frigerio, Lorenzo
author_facet Brown, Daniel
Feeney, Mistianne
Ahmadi, Mathin
Lonoce, Chiara
Sajari, Roslinda
Di Cola, Alessandra
Frigerio, Lorenzo
author_sort Brown, Daniel
collection PubMed
description Natural rubber (polyisoprene) from the rubber tree Hevea brasiliensis is synthesized by specialized cells called laticifers. It is not clear how rubber particles arise, although one hypothesis is that they derive from the endoplasmic reticulum (ER) membrane. Here we cloned the genes encoding four key proteins found in association with rubber particles and studied their intracellular localization by transient expression in Nicotiana benthamiana leaves. We show that, while the cis-prenyltransferase (CPT), responsible for the synthesis of long polyisoprene chains, is a soluble, cytosolic protein, other rubber particle proteins such as rubber elongation factor (REF), small rubber particle protein (SRPP) and Hevea rubber transferase 1-REF bridging protein (HRBP) are associated with the endoplasmic reticulum (ER). We also show that SRPP can recruit CPT to the ER and that interaction of CPT with HRBP leads to both proteins relocating to the plasma membrane. We discuss these results in the context of the biogenesis of rubber particles.
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spelling pubmed-58538942018-07-25 Subcellular localization and interactions among rubber particle proteins from Hevea brasiliensis Brown, Daniel Feeney, Mistianne Ahmadi, Mathin Lonoce, Chiara Sajari, Roslinda Di Cola, Alessandra Frigerio, Lorenzo J Exp Bot Research Papers Natural rubber (polyisoprene) from the rubber tree Hevea brasiliensis is synthesized by specialized cells called laticifers. It is not clear how rubber particles arise, although one hypothesis is that they derive from the endoplasmic reticulum (ER) membrane. Here we cloned the genes encoding four key proteins found in association with rubber particles and studied their intracellular localization by transient expression in Nicotiana benthamiana leaves. We show that, while the cis-prenyltransferase (CPT), responsible for the synthesis of long polyisoprene chains, is a soluble, cytosolic protein, other rubber particle proteins such as rubber elongation factor (REF), small rubber particle protein (SRPP) and Hevea rubber transferase 1-REF bridging protein (HRBP) are associated with the endoplasmic reticulum (ER). We also show that SRPP can recruit CPT to the ER and that interaction of CPT with HRBP leads to both proteins relocating to the plasma membrane. We discuss these results in the context of the biogenesis of rubber particles. Oxford University Press 2017-11-02 2017-09-25 /pmc/articles/PMC5853894/ /pubmed/29036360 http://dx.doi.org/10.1093/jxb/erx331 Text en © The Author 2017. Published by Oxford University Press on behalf of the Society for Experimental Biology. http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Papers
Brown, Daniel
Feeney, Mistianne
Ahmadi, Mathin
Lonoce, Chiara
Sajari, Roslinda
Di Cola, Alessandra
Frigerio, Lorenzo
Subcellular localization and interactions among rubber particle proteins from Hevea brasiliensis
title Subcellular localization and interactions among rubber particle proteins from Hevea brasiliensis
title_full Subcellular localization and interactions among rubber particle proteins from Hevea brasiliensis
title_fullStr Subcellular localization and interactions among rubber particle proteins from Hevea brasiliensis
title_full_unstemmed Subcellular localization and interactions among rubber particle proteins from Hevea brasiliensis
title_short Subcellular localization and interactions among rubber particle proteins from Hevea brasiliensis
title_sort subcellular localization and interactions among rubber particle proteins from hevea brasiliensis
topic Research Papers
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5853894/
https://www.ncbi.nlm.nih.gov/pubmed/29036360
http://dx.doi.org/10.1093/jxb/erx331
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