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Root transcriptomic responses of grafted grapevines to heterogeneous nitrogen availability depend on rootstock genotype

In many fruit species, including grapevine, grafting is used to improve scion productivity and quality and to adapt the plant to environmental conditions. However, the mechanisms underlying the rootstock control of scion development are still poorly understood. The ability of rootstocks to regulate...

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Autores principales: Cochetel, Noé, Escudié, Frédéric, Cookson, Sarah Jane, Dai, Zhanwu, Vivin, Philippe, Bert, Pierre-François, Muñoz, Mindy Stephania, Delrot, Serge, Klopp, Christophe, Ollat, Nathalie, Lauvergeat, Virginie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2017
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5854021/
https://www.ncbi.nlm.nih.gov/pubmed/28922755
http://dx.doi.org/10.1093/jxb/erx224
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author Cochetel, Noé
Escudié, Frédéric
Cookson, Sarah Jane
Dai, Zhanwu
Vivin, Philippe
Bert, Pierre-François
Muñoz, Mindy Stephania
Delrot, Serge
Klopp, Christophe
Ollat, Nathalie
Lauvergeat, Virginie
author_facet Cochetel, Noé
Escudié, Frédéric
Cookson, Sarah Jane
Dai, Zhanwu
Vivin, Philippe
Bert, Pierre-François
Muñoz, Mindy Stephania
Delrot, Serge
Klopp, Christophe
Ollat, Nathalie
Lauvergeat, Virginie
author_sort Cochetel, Noé
collection PubMed
description In many fruit species, including grapevine, grafting is used to improve scion productivity and quality and to adapt the plant to environmental conditions. However, the mechanisms underlying the rootstock control of scion development are still poorly understood. The ability of rootstocks to regulate nitrogen uptake and assimilation may contribute to this control. A split-root system was used to grow heterografted grapevines and to investigate the molecular responses to changes in nitrate availability of two rootstocks known to affect scion growth differently. Transcriptome profiling by RNA sequencing was performed on root samples collected 3 and 24 h after nitrogen supply. The results demonstrated a common response involving nitrogen-related genes, as well as a more pronounced transcriptomic reprogramming in the genotype conferring the lower scion growth. A weighted gene co-expression network analysis allowed the identification of co-regulated gene modules, suggesting a role for nitrate transporter 2 family genes and some transcription factors as main actors controlling this genotype-dependent response to heterogeneous nitrogen supply. The relationship between nitrate, ethylene, and strigolactone hormonal pathways was found to differ between the two genotypes. These findings indicated that the genotypes responded differently to heterogeneous nitrogen availability, and this may contribute to their contrasting effect on scion growth.
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spelling pubmed-58540212018-07-27 Root transcriptomic responses of grafted grapevines to heterogeneous nitrogen availability depend on rootstock genotype Cochetel, Noé Escudié, Frédéric Cookson, Sarah Jane Dai, Zhanwu Vivin, Philippe Bert, Pierre-François Muñoz, Mindy Stephania Delrot, Serge Klopp, Christophe Ollat, Nathalie Lauvergeat, Virginie J Exp Bot Research Papers In many fruit species, including grapevine, grafting is used to improve scion productivity and quality and to adapt the plant to environmental conditions. However, the mechanisms underlying the rootstock control of scion development are still poorly understood. The ability of rootstocks to regulate nitrogen uptake and assimilation may contribute to this control. A split-root system was used to grow heterografted grapevines and to investigate the molecular responses to changes in nitrate availability of two rootstocks known to affect scion growth differently. Transcriptome profiling by RNA sequencing was performed on root samples collected 3 and 24 h after nitrogen supply. The results demonstrated a common response involving nitrogen-related genes, as well as a more pronounced transcriptomic reprogramming in the genotype conferring the lower scion growth. A weighted gene co-expression network analysis allowed the identification of co-regulated gene modules, suggesting a role for nitrate transporter 2 family genes and some transcription factors as main actors controlling this genotype-dependent response to heterogeneous nitrogen supply. The relationship between nitrate, ethylene, and strigolactone hormonal pathways was found to differ between the two genotypes. These findings indicated that the genotypes responded differently to heterogeneous nitrogen availability, and this may contribute to their contrasting effect on scion growth. Oxford University Press 2017-07-10 2017-07-11 /pmc/articles/PMC5854021/ /pubmed/28922755 http://dx.doi.org/10.1093/jxb/erx224 Text en © The Author 2017. Published by Oxford University Press on behalf of the Society for Experimental Biology. http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Papers
Cochetel, Noé
Escudié, Frédéric
Cookson, Sarah Jane
Dai, Zhanwu
Vivin, Philippe
Bert, Pierre-François
Muñoz, Mindy Stephania
Delrot, Serge
Klopp, Christophe
Ollat, Nathalie
Lauvergeat, Virginie
Root transcriptomic responses of grafted grapevines to heterogeneous nitrogen availability depend on rootstock genotype
title Root transcriptomic responses of grafted grapevines to heterogeneous nitrogen availability depend on rootstock genotype
title_full Root transcriptomic responses of grafted grapevines to heterogeneous nitrogen availability depend on rootstock genotype
title_fullStr Root transcriptomic responses of grafted grapevines to heterogeneous nitrogen availability depend on rootstock genotype
title_full_unstemmed Root transcriptomic responses of grafted grapevines to heterogeneous nitrogen availability depend on rootstock genotype
title_short Root transcriptomic responses of grafted grapevines to heterogeneous nitrogen availability depend on rootstock genotype
title_sort root transcriptomic responses of grafted grapevines to heterogeneous nitrogen availability depend on rootstock genotype
topic Research Papers
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5854021/
https://www.ncbi.nlm.nih.gov/pubmed/28922755
http://dx.doi.org/10.1093/jxb/erx224
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