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GDP-L-fucose is required for boundary definition in plants

The CUP-SHAPED COTYLEDON (CUC) transcription factors control plant boundary formation, thus allowing the emergence of novel growth axes. While the developmental roles of the CUC genes in different organs and across species are well characterized, upstream and downstream events that contribute to the...

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Autores principales: Gonçalves, Beatriz, Maugarny-Calès, Aude, Adroher, Bernard, Cortizo, Millán, Borrega, Nero, Blein, Thomas, Hasson, Alice, Gineau, Emilie, Mouille, Grégory, Laufs, Patrick, Arnaud, Nicolas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5854112/
https://www.ncbi.nlm.nih.gov/pubmed/29186469
http://dx.doi.org/10.1093/jxb/erx402
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author Gonçalves, Beatriz
Maugarny-Calès, Aude
Adroher, Bernard
Cortizo, Millán
Borrega, Nero
Blein, Thomas
Hasson, Alice
Gineau, Emilie
Mouille, Grégory
Laufs, Patrick
Arnaud, Nicolas
author_facet Gonçalves, Beatriz
Maugarny-Calès, Aude
Adroher, Bernard
Cortizo, Millán
Borrega, Nero
Blein, Thomas
Hasson, Alice
Gineau, Emilie
Mouille, Grégory
Laufs, Patrick
Arnaud, Nicolas
author_sort Gonçalves, Beatriz
collection PubMed
description The CUP-SHAPED COTYLEDON (CUC) transcription factors control plant boundary formation, thus allowing the emergence of novel growth axes. While the developmental roles of the CUC genes in different organs and across species are well characterized, upstream and downstream events that contribute to their function are still poorly understood. To identify new players in this network, we performed a suppressor screen of CUC2g-m4, a line overexpressing CUC2 that has highly serrated leaves. We identified a mutation that simplifies leaf shape and affects MURUS1 (MUR1), which is responsible for GDP-L-fucose production. Using detailed morphometric analysis, we show that GDP-L-fucose has an essential role in leaf shape acquisition by sustaining differential growth at the leaf margins. Accordingly, reduced CUC2 expression levels are observed in mur1 leaves. Furthermore, genetic analyses reveal a conserved role for GDP-L-fucose in different developmental contexts where it contributes to organ separation in the same pathway as CUC2. Taken together, our results reveal that GDP-L-fucose is necessary for proper establishment of boundary domains in various developmental contexts.
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spelling pubmed-58541122018-07-25 GDP-L-fucose is required for boundary definition in plants Gonçalves, Beatriz Maugarny-Calès, Aude Adroher, Bernard Cortizo, Millán Borrega, Nero Blein, Thomas Hasson, Alice Gineau, Emilie Mouille, Grégory Laufs, Patrick Arnaud, Nicolas J Exp Bot Research Papers The CUP-SHAPED COTYLEDON (CUC) transcription factors control plant boundary formation, thus allowing the emergence of novel growth axes. While the developmental roles of the CUC genes in different organs and across species are well characterized, upstream and downstream events that contribute to their function are still poorly understood. To identify new players in this network, we performed a suppressor screen of CUC2g-m4, a line overexpressing CUC2 that has highly serrated leaves. We identified a mutation that simplifies leaf shape and affects MURUS1 (MUR1), which is responsible for GDP-L-fucose production. Using detailed morphometric analysis, we show that GDP-L-fucose has an essential role in leaf shape acquisition by sustaining differential growth at the leaf margins. Accordingly, reduced CUC2 expression levels are observed in mur1 leaves. Furthermore, genetic analyses reveal a conserved role for GDP-L-fucose in different developmental contexts where it contributes to organ separation in the same pathway as CUC2. Taken together, our results reveal that GDP-L-fucose is necessary for proper establishment of boundary domains in various developmental contexts. Oxford University Press 2017-12-16 2017-11-25 /pmc/articles/PMC5854112/ /pubmed/29186469 http://dx.doi.org/10.1093/jxb/erx402 Text en © The Author 2017. Published by Oxford University Press on behalf of the Society for Experimental Biology. http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Papers
Gonçalves, Beatriz
Maugarny-Calès, Aude
Adroher, Bernard
Cortizo, Millán
Borrega, Nero
Blein, Thomas
Hasson, Alice
Gineau, Emilie
Mouille, Grégory
Laufs, Patrick
Arnaud, Nicolas
GDP-L-fucose is required for boundary definition in plants
title GDP-L-fucose is required for boundary definition in plants
title_full GDP-L-fucose is required for boundary definition in plants
title_fullStr GDP-L-fucose is required for boundary definition in plants
title_full_unstemmed GDP-L-fucose is required for boundary definition in plants
title_short GDP-L-fucose is required for boundary definition in plants
title_sort gdp-l-fucose is required for boundary definition in plants
topic Research Papers
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5854112/
https://www.ncbi.nlm.nih.gov/pubmed/29186469
http://dx.doi.org/10.1093/jxb/erx402
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