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DOFT and DOFTIP1 affect reproductive development in the orchid Dendrobium Chao Praya Smile
FLOWERING LOCUS T (FT) in Arabidopsis encodes the florigen that moves from leaves to the shoot apical meristem to induce flowering, and this is partly mediated by FT-INTERACTING PROTEIN 1 (FTIP1). Although FT orthologs have been identified in some flowering plants, their endogenous roles in Orchidac...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5854133/ https://www.ncbi.nlm.nih.gov/pubmed/29186512 http://dx.doi.org/10.1093/jxb/erx400 |
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author | Wang, Yanwen Liu, Lu Song, Shiyong Li, Yan Shen, Lisha Yu, Hao |
author_facet | Wang, Yanwen Liu, Lu Song, Shiyong Li, Yan Shen, Lisha Yu, Hao |
author_sort | Wang, Yanwen |
collection | PubMed |
description | FLOWERING LOCUS T (FT) in Arabidopsis encodes the florigen that moves from leaves to the shoot apical meristem to induce flowering, and this is partly mediated by FT-INTERACTING PROTEIN 1 (FTIP1). Although FT orthologs have been identified in some flowering plants, their endogenous roles in Orchidaceae, which is one of the largest families of flowering plants, are still largely unknown. In this study, we show that DOFT and DOFTIP1, the orchid orthologs of FT and FTIP1, respectively, play important roles in promoting flowering in the orchid Dendrobium Chao Praya Smile. Expression of DOFT and DOFTIP1 increases in whole plantlets during the transition from vegetative to reproductive development. Both transcripts are present in significant levels in reproductive organs, including inflorescence apices, stems, floral buds, and open flowers. Through successful generation of transgenic orchids, we have revealed that overexpression or down-regulation of DOFT accelerates or delays flowering, respectively, while alteration of DOFT expression also greatly affects pseudobulb formation and flower development. In common with their counterparts in Arabidopsis and rice, DOFTIP1 interacts with DOFT and affects flowering time in orchids. Our results suggest that while DOFT and DOFTIP1 play evolutionarily conserved roles in promoting flowering, DOFT may have evolved with hitherto unknown functions pertaining to the regulation of storage organs and flower development in the Orchidaceae family. |
format | Online Article Text |
id | pubmed-5854133 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-58541332018-07-27 DOFT and DOFTIP1 affect reproductive development in the orchid Dendrobium Chao Praya Smile Wang, Yanwen Liu, Lu Song, Shiyong Li, Yan Shen, Lisha Yu, Hao J Exp Bot Research Papers FLOWERING LOCUS T (FT) in Arabidopsis encodes the florigen that moves from leaves to the shoot apical meristem to induce flowering, and this is partly mediated by FT-INTERACTING PROTEIN 1 (FTIP1). Although FT orthologs have been identified in some flowering plants, their endogenous roles in Orchidaceae, which is one of the largest families of flowering plants, are still largely unknown. In this study, we show that DOFT and DOFTIP1, the orchid orthologs of FT and FTIP1, respectively, play important roles in promoting flowering in the orchid Dendrobium Chao Praya Smile. Expression of DOFT and DOFTIP1 increases in whole plantlets during the transition from vegetative to reproductive development. Both transcripts are present in significant levels in reproductive organs, including inflorescence apices, stems, floral buds, and open flowers. Through successful generation of transgenic orchids, we have revealed that overexpression or down-regulation of DOFT accelerates or delays flowering, respectively, while alteration of DOFT expression also greatly affects pseudobulb formation and flower development. In common with their counterparts in Arabidopsis and rice, DOFTIP1 interacts with DOFT and affects flowering time in orchids. Our results suggest that while DOFT and DOFTIP1 play evolutionarily conserved roles in promoting flowering, DOFT may have evolved with hitherto unknown functions pertaining to the regulation of storage organs and flower development in the Orchidaceae family. Oxford University Press 2017-12-16 2017-11-24 /pmc/articles/PMC5854133/ /pubmed/29186512 http://dx.doi.org/10.1093/jxb/erx400 Text en © The Author 2017. Published by Oxford University Press on behalf of the Society for Experimental Biology. http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Papers Wang, Yanwen Liu, Lu Song, Shiyong Li, Yan Shen, Lisha Yu, Hao DOFT and DOFTIP1 affect reproductive development in the orchid Dendrobium Chao Praya Smile |
title |
DOFT and DOFTIP1 affect reproductive development in the orchid Dendrobium Chao Praya Smile |
title_full |
DOFT and DOFTIP1 affect reproductive development in the orchid Dendrobium Chao Praya Smile |
title_fullStr |
DOFT and DOFTIP1 affect reproductive development in the orchid Dendrobium Chao Praya Smile |
title_full_unstemmed |
DOFT and DOFTIP1 affect reproductive development in the orchid Dendrobium Chao Praya Smile |
title_short |
DOFT and DOFTIP1 affect reproductive development in the orchid Dendrobium Chao Praya Smile |
title_sort | doft and doftip1 affect reproductive development in the orchid dendrobium chao praya smile |
topic | Research Papers |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5854133/ https://www.ncbi.nlm.nih.gov/pubmed/29186512 http://dx.doi.org/10.1093/jxb/erx400 |
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