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Antifungal activity, main active components and mechanism of Curcuma longa extract against Fusarium graminearum
Curcuma longa possesses powerful antifungal activity, as demonstrated in many studies. In this study, the antifungal spectrum of Curcuma longa alcohol extract was determined, and the resulting EC50 values (mg/mL) of its extract on eleven fungi, including Fusarium graminearum, Fusarium chlamydosporum...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5854386/ https://www.ncbi.nlm.nih.gov/pubmed/29543859 http://dx.doi.org/10.1371/journal.pone.0194284 |
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author | Chen, Ciqiong Long, Li Zhang, Fusheng Chen, Qin Chen, Cheng Yu, Xiaorui Liu, Qingya Bao, Jinku Long, Zhangfu |
author_facet | Chen, Ciqiong Long, Li Zhang, Fusheng Chen, Qin Chen, Cheng Yu, Xiaorui Liu, Qingya Bao, Jinku Long, Zhangfu |
author_sort | Chen, Ciqiong |
collection | PubMed |
description | Curcuma longa possesses powerful antifungal activity, as demonstrated in many studies. In this study, the antifungal spectrum of Curcuma longa alcohol extract was determined, and the resulting EC50 values (mg/mL) of its extract on eleven fungi, including Fusarium graminearum, Fusarium chlamydosporum, Alternaria alternate, Fusarium tricinctum, Sclerotinia sclerotiorum, Botrytis cinerea, Fusarium culmorum, Rhizopus oryzae, Cladosporium cladosporioides, Fusarium oxysporum and Colletotrichum higginsianum, were 0.1088, 0.1742, 0.1888, 0.2547, 0.3135, 0.3825, 0.4229, 1.2086, 4.5176, 3.8833 and 5.0183, respectively. Among them, F. graminearum was selected to determine the inhibitory effects of the compounds (including curdione, isocurcumenol, curcumenol, curzerene, β-elemene, curcumin, germacrone and curcumol) derived from Curcuma longa. In addition, the antifungal activities of curdione, curcumenol, curzerene, curcumol and isocurcumenol and the synergies of the complexes of curdione and seven other chemicals were investigated. Differential proteomics of F. graminearum was also compared, and at least 2021 reproducible protein spots were identified. Among these spots, 46 were classified as differentially expressed proteins, and these proteins are involved in energy metabolism, tRNA synthesis and glucose metabolism. Furthermore, several fungal physiological differences were also analysed. The antifungal effect included fungal cell membrane disruption and inhibition of ergosterol synthesis, respiration, succinate dehydrogenase (SDH) and NADH oxidase. |
format | Online Article Text |
id | pubmed-5854386 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-58543862018-03-28 Antifungal activity, main active components and mechanism of Curcuma longa extract against Fusarium graminearum Chen, Ciqiong Long, Li Zhang, Fusheng Chen, Qin Chen, Cheng Yu, Xiaorui Liu, Qingya Bao, Jinku Long, Zhangfu PLoS One Research Article Curcuma longa possesses powerful antifungal activity, as demonstrated in many studies. In this study, the antifungal spectrum of Curcuma longa alcohol extract was determined, and the resulting EC50 values (mg/mL) of its extract on eleven fungi, including Fusarium graminearum, Fusarium chlamydosporum, Alternaria alternate, Fusarium tricinctum, Sclerotinia sclerotiorum, Botrytis cinerea, Fusarium culmorum, Rhizopus oryzae, Cladosporium cladosporioides, Fusarium oxysporum and Colletotrichum higginsianum, were 0.1088, 0.1742, 0.1888, 0.2547, 0.3135, 0.3825, 0.4229, 1.2086, 4.5176, 3.8833 and 5.0183, respectively. Among them, F. graminearum was selected to determine the inhibitory effects of the compounds (including curdione, isocurcumenol, curcumenol, curzerene, β-elemene, curcumin, germacrone and curcumol) derived from Curcuma longa. In addition, the antifungal activities of curdione, curcumenol, curzerene, curcumol and isocurcumenol and the synergies of the complexes of curdione and seven other chemicals were investigated. Differential proteomics of F. graminearum was also compared, and at least 2021 reproducible protein spots were identified. Among these spots, 46 were classified as differentially expressed proteins, and these proteins are involved in energy metabolism, tRNA synthesis and glucose metabolism. Furthermore, several fungal physiological differences were also analysed. The antifungal effect included fungal cell membrane disruption and inhibition of ergosterol synthesis, respiration, succinate dehydrogenase (SDH) and NADH oxidase. Public Library of Science 2018-03-15 /pmc/articles/PMC5854386/ /pubmed/29543859 http://dx.doi.org/10.1371/journal.pone.0194284 Text en © 2018 Chen et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Chen, Ciqiong Long, Li Zhang, Fusheng Chen, Qin Chen, Cheng Yu, Xiaorui Liu, Qingya Bao, Jinku Long, Zhangfu Antifungal activity, main active components and mechanism of Curcuma longa extract against Fusarium graminearum |
title | Antifungal activity, main active components and mechanism of Curcuma longa extract against Fusarium graminearum |
title_full | Antifungal activity, main active components and mechanism of Curcuma longa extract against Fusarium graminearum |
title_fullStr | Antifungal activity, main active components and mechanism of Curcuma longa extract against Fusarium graminearum |
title_full_unstemmed | Antifungal activity, main active components and mechanism of Curcuma longa extract against Fusarium graminearum |
title_short | Antifungal activity, main active components and mechanism of Curcuma longa extract against Fusarium graminearum |
title_sort | antifungal activity, main active components and mechanism of curcuma longa extract against fusarium graminearum |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5854386/ https://www.ncbi.nlm.nih.gov/pubmed/29543859 http://dx.doi.org/10.1371/journal.pone.0194284 |
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