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Altered vector competence in an experimental mosquito-mouse transmission model of Zika infection
Few animal models of Zika virus (ZIKV) infection have incorporated arthropod-borne transmission. Here, we establish an Aedes aegypti mosquito model of ZIKV infection of mice, and demonstrate altered vector competency among three strains, (Orlando, ORL, Ho Chi Minh, HCM, and Patilas, PAT). All strain...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5854422/ https://www.ncbi.nlm.nih.gov/pubmed/29505571 http://dx.doi.org/10.1371/journal.pntd.0006350 |
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author | Uraki, Ryuta Hastings, Andrew K. Gloria-Soria, Andrea Powell, Jeffrey R. Fikrig, Erol |
author_facet | Uraki, Ryuta Hastings, Andrew K. Gloria-Soria, Andrea Powell, Jeffrey R. Fikrig, Erol |
author_sort | Uraki, Ryuta |
collection | PubMed |
description | Few animal models of Zika virus (ZIKV) infection have incorporated arthropod-borne transmission. Here, we establish an Aedes aegypti mosquito model of ZIKV infection of mice, and demonstrate altered vector competency among three strains, (Orlando, ORL, Ho Chi Minh, HCM, and Patilas, PAT). All strains acquired ZIKV in their midguts after a blood meal from infected mice, but ZIKV transmission only occurred in mice fed upon by HCM, and to a lesser extent PAT, but not ORL, mosquitoes. This defect in transmission from ORL or PAT mosquitoes was overcome by intrathoracic injection of ZIKV into mosquito. Genetic analysis revealed significant diversity among these strains, suggesting a genetic basis for differences in ability for mosquito strains to transmit ZIKV. The intrathoracic injection mosquito-mouse transmission model is critical to understanding the influence of mosquitoes on ZIKV transmission, infectivity and pathogenesis in the vertebrate host, and represents a natural transmission route for testing vaccines and therapeutics. |
format | Online Article Text |
id | pubmed-5854422 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-58544222018-03-28 Altered vector competence in an experimental mosquito-mouse transmission model of Zika infection Uraki, Ryuta Hastings, Andrew K. Gloria-Soria, Andrea Powell, Jeffrey R. Fikrig, Erol PLoS Negl Trop Dis Research Article Few animal models of Zika virus (ZIKV) infection have incorporated arthropod-borne transmission. Here, we establish an Aedes aegypti mosquito model of ZIKV infection of mice, and demonstrate altered vector competency among three strains, (Orlando, ORL, Ho Chi Minh, HCM, and Patilas, PAT). All strains acquired ZIKV in their midguts after a blood meal from infected mice, but ZIKV transmission only occurred in mice fed upon by HCM, and to a lesser extent PAT, but not ORL, mosquitoes. This defect in transmission from ORL or PAT mosquitoes was overcome by intrathoracic injection of ZIKV into mosquito. Genetic analysis revealed significant diversity among these strains, suggesting a genetic basis for differences in ability for mosquito strains to transmit ZIKV. The intrathoracic injection mosquito-mouse transmission model is critical to understanding the influence of mosquitoes on ZIKV transmission, infectivity and pathogenesis in the vertebrate host, and represents a natural transmission route for testing vaccines and therapeutics. Public Library of Science 2018-03-05 /pmc/articles/PMC5854422/ /pubmed/29505571 http://dx.doi.org/10.1371/journal.pntd.0006350 Text en © 2018 Uraki et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Uraki, Ryuta Hastings, Andrew K. Gloria-Soria, Andrea Powell, Jeffrey R. Fikrig, Erol Altered vector competence in an experimental mosquito-mouse transmission model of Zika infection |
title | Altered vector competence in an experimental mosquito-mouse transmission model of Zika infection |
title_full | Altered vector competence in an experimental mosquito-mouse transmission model of Zika infection |
title_fullStr | Altered vector competence in an experimental mosquito-mouse transmission model of Zika infection |
title_full_unstemmed | Altered vector competence in an experimental mosquito-mouse transmission model of Zika infection |
title_short | Altered vector competence in an experimental mosquito-mouse transmission model of Zika infection |
title_sort | altered vector competence in an experimental mosquito-mouse transmission model of zika infection |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5854422/ https://www.ncbi.nlm.nih.gov/pubmed/29505571 http://dx.doi.org/10.1371/journal.pntd.0006350 |
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