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Altered vector competence in an experimental mosquito-mouse transmission model of Zika infection

Few animal models of Zika virus (ZIKV) infection have incorporated arthropod-borne transmission. Here, we establish an Aedes aegypti mosquito model of ZIKV infection of mice, and demonstrate altered vector competency among three strains, (Orlando, ORL, Ho Chi Minh, HCM, and Patilas, PAT). All strain...

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Autores principales: Uraki, Ryuta, Hastings, Andrew K., Gloria-Soria, Andrea, Powell, Jeffrey R., Fikrig, Erol
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5854422/
https://www.ncbi.nlm.nih.gov/pubmed/29505571
http://dx.doi.org/10.1371/journal.pntd.0006350
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author Uraki, Ryuta
Hastings, Andrew K.
Gloria-Soria, Andrea
Powell, Jeffrey R.
Fikrig, Erol
author_facet Uraki, Ryuta
Hastings, Andrew K.
Gloria-Soria, Andrea
Powell, Jeffrey R.
Fikrig, Erol
author_sort Uraki, Ryuta
collection PubMed
description Few animal models of Zika virus (ZIKV) infection have incorporated arthropod-borne transmission. Here, we establish an Aedes aegypti mosquito model of ZIKV infection of mice, and demonstrate altered vector competency among three strains, (Orlando, ORL, Ho Chi Minh, HCM, and Patilas, PAT). All strains acquired ZIKV in their midguts after a blood meal from infected mice, but ZIKV transmission only occurred in mice fed upon by HCM, and to a lesser extent PAT, but not ORL, mosquitoes. This defect in transmission from ORL or PAT mosquitoes was overcome by intrathoracic injection of ZIKV into mosquito. Genetic analysis revealed significant diversity among these strains, suggesting a genetic basis for differences in ability for mosquito strains to transmit ZIKV. The intrathoracic injection mosquito-mouse transmission model is critical to understanding the influence of mosquitoes on ZIKV transmission, infectivity and pathogenesis in the vertebrate host, and represents a natural transmission route for testing vaccines and therapeutics.
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spelling pubmed-58544222018-03-28 Altered vector competence in an experimental mosquito-mouse transmission model of Zika infection Uraki, Ryuta Hastings, Andrew K. Gloria-Soria, Andrea Powell, Jeffrey R. Fikrig, Erol PLoS Negl Trop Dis Research Article Few animal models of Zika virus (ZIKV) infection have incorporated arthropod-borne transmission. Here, we establish an Aedes aegypti mosquito model of ZIKV infection of mice, and demonstrate altered vector competency among three strains, (Orlando, ORL, Ho Chi Minh, HCM, and Patilas, PAT). All strains acquired ZIKV in their midguts after a blood meal from infected mice, but ZIKV transmission only occurred in mice fed upon by HCM, and to a lesser extent PAT, but not ORL, mosquitoes. This defect in transmission from ORL or PAT mosquitoes was overcome by intrathoracic injection of ZIKV into mosquito. Genetic analysis revealed significant diversity among these strains, suggesting a genetic basis for differences in ability for mosquito strains to transmit ZIKV. The intrathoracic injection mosquito-mouse transmission model is critical to understanding the influence of mosquitoes on ZIKV transmission, infectivity and pathogenesis in the vertebrate host, and represents a natural transmission route for testing vaccines and therapeutics. Public Library of Science 2018-03-05 /pmc/articles/PMC5854422/ /pubmed/29505571 http://dx.doi.org/10.1371/journal.pntd.0006350 Text en © 2018 Uraki et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Uraki, Ryuta
Hastings, Andrew K.
Gloria-Soria, Andrea
Powell, Jeffrey R.
Fikrig, Erol
Altered vector competence in an experimental mosquito-mouse transmission model of Zika infection
title Altered vector competence in an experimental mosquito-mouse transmission model of Zika infection
title_full Altered vector competence in an experimental mosquito-mouse transmission model of Zika infection
title_fullStr Altered vector competence in an experimental mosquito-mouse transmission model of Zika infection
title_full_unstemmed Altered vector competence in an experimental mosquito-mouse transmission model of Zika infection
title_short Altered vector competence in an experimental mosquito-mouse transmission model of Zika infection
title_sort altered vector competence in an experimental mosquito-mouse transmission model of zika infection
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5854422/
https://www.ncbi.nlm.nih.gov/pubmed/29505571
http://dx.doi.org/10.1371/journal.pntd.0006350
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