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MreB filaments align along greatest principal membrane curvature to orient cell wall synthesis
MreB is essential for rod shape in many bacteria. Membrane-associated MreB filaments move around the rod circumference, helping to insert cell wall in the radial direction to reinforce rod shape. To understand how oriented MreB motion arises, we altered the shape of Bacillus subtilis. MreB motion is...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
eLife Sciences Publications, Ltd
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5854468/ https://www.ncbi.nlm.nih.gov/pubmed/29469806 http://dx.doi.org/10.7554/eLife.32471 |
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author | Hussain, Saman Wivagg, Carl N Szwedziak, Piotr Wong, Felix Schaefer, Kaitlin Izoré, Thierry Renner, Lars D Holmes, Matthew J Sun, Yingjie Bisson-Filho, Alexandre W Walker, Suzanne Amir, Ariel Löwe, Jan Garner, Ethan C |
author_facet | Hussain, Saman Wivagg, Carl N Szwedziak, Piotr Wong, Felix Schaefer, Kaitlin Izoré, Thierry Renner, Lars D Holmes, Matthew J Sun, Yingjie Bisson-Filho, Alexandre W Walker, Suzanne Amir, Ariel Löwe, Jan Garner, Ethan C |
author_sort | Hussain, Saman |
collection | PubMed |
description | MreB is essential for rod shape in many bacteria. Membrane-associated MreB filaments move around the rod circumference, helping to insert cell wall in the radial direction to reinforce rod shape. To understand how oriented MreB motion arises, we altered the shape of Bacillus subtilis. MreB motion is isotropic in round cells, and orientation is restored when rod shape is externally imposed. Stationary filaments orient within protoplasts, and purified MreB tubulates liposomes in vitro, orienting within tubes. Together, this demonstrates MreB orients along the greatest principal membrane curvature, a conclusion supported with biophysical modeling. We observed that spherical cells regenerate into rods in a local, self-reinforcing manner: rapidly propagating rods emerge from small bulges, exhibiting oriented MreB motion. We propose that the coupling of MreB filament alignment to shape-reinforcing peptidoglycan synthesis creates a locally-acting, self-organizing mechanism allowing the rapid establishment and stable maintenance of emergent rod shape. |
format | Online Article Text |
id | pubmed-5854468 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | eLife Sciences Publications, Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-58544682018-03-20 MreB filaments align along greatest principal membrane curvature to orient cell wall synthesis Hussain, Saman Wivagg, Carl N Szwedziak, Piotr Wong, Felix Schaefer, Kaitlin Izoré, Thierry Renner, Lars D Holmes, Matthew J Sun, Yingjie Bisson-Filho, Alexandre W Walker, Suzanne Amir, Ariel Löwe, Jan Garner, Ethan C eLife Microbiology and Infectious Disease MreB is essential for rod shape in many bacteria. Membrane-associated MreB filaments move around the rod circumference, helping to insert cell wall in the radial direction to reinforce rod shape. To understand how oriented MreB motion arises, we altered the shape of Bacillus subtilis. MreB motion is isotropic in round cells, and orientation is restored when rod shape is externally imposed. Stationary filaments orient within protoplasts, and purified MreB tubulates liposomes in vitro, orienting within tubes. Together, this demonstrates MreB orients along the greatest principal membrane curvature, a conclusion supported with biophysical modeling. We observed that spherical cells regenerate into rods in a local, self-reinforcing manner: rapidly propagating rods emerge from small bulges, exhibiting oriented MreB motion. We propose that the coupling of MreB filament alignment to shape-reinforcing peptidoglycan synthesis creates a locally-acting, self-organizing mechanism allowing the rapid establishment and stable maintenance of emergent rod shape. eLife Sciences Publications, Ltd 2018-02-22 /pmc/articles/PMC5854468/ /pubmed/29469806 http://dx.doi.org/10.7554/eLife.32471 Text en © 2018, Hussain et al http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited. |
spellingShingle | Microbiology and Infectious Disease Hussain, Saman Wivagg, Carl N Szwedziak, Piotr Wong, Felix Schaefer, Kaitlin Izoré, Thierry Renner, Lars D Holmes, Matthew J Sun, Yingjie Bisson-Filho, Alexandre W Walker, Suzanne Amir, Ariel Löwe, Jan Garner, Ethan C MreB filaments align along greatest principal membrane curvature to orient cell wall synthesis |
title | MreB filaments align along greatest principal membrane curvature to orient cell wall synthesis |
title_full | MreB filaments align along greatest principal membrane curvature to orient cell wall synthesis |
title_fullStr | MreB filaments align along greatest principal membrane curvature to orient cell wall synthesis |
title_full_unstemmed | MreB filaments align along greatest principal membrane curvature to orient cell wall synthesis |
title_short | MreB filaments align along greatest principal membrane curvature to orient cell wall synthesis |
title_sort | mreb filaments align along greatest principal membrane curvature to orient cell wall synthesis |
topic | Microbiology and Infectious Disease |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5854468/ https://www.ncbi.nlm.nih.gov/pubmed/29469806 http://dx.doi.org/10.7554/eLife.32471 |
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