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MreB filaments align along greatest principal membrane curvature to orient cell wall synthesis

MreB is essential for rod shape in many bacteria. Membrane-associated MreB filaments move around the rod circumference, helping to insert cell wall in the radial direction to reinforce rod shape. To understand how oriented MreB motion arises, we altered the shape of Bacillus subtilis. MreB motion is...

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Autores principales: Hussain, Saman, Wivagg, Carl N, Szwedziak, Piotr, Wong, Felix, Schaefer, Kaitlin, Izoré, Thierry, Renner, Lars D, Holmes, Matthew J, Sun, Yingjie, Bisson-Filho, Alexandre W, Walker, Suzanne, Amir, Ariel, Löwe, Jan, Garner, Ethan C
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5854468/
https://www.ncbi.nlm.nih.gov/pubmed/29469806
http://dx.doi.org/10.7554/eLife.32471
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author Hussain, Saman
Wivagg, Carl N
Szwedziak, Piotr
Wong, Felix
Schaefer, Kaitlin
Izoré, Thierry
Renner, Lars D
Holmes, Matthew J
Sun, Yingjie
Bisson-Filho, Alexandre W
Walker, Suzanne
Amir, Ariel
Löwe, Jan
Garner, Ethan C
author_facet Hussain, Saman
Wivagg, Carl N
Szwedziak, Piotr
Wong, Felix
Schaefer, Kaitlin
Izoré, Thierry
Renner, Lars D
Holmes, Matthew J
Sun, Yingjie
Bisson-Filho, Alexandre W
Walker, Suzanne
Amir, Ariel
Löwe, Jan
Garner, Ethan C
author_sort Hussain, Saman
collection PubMed
description MreB is essential for rod shape in many bacteria. Membrane-associated MreB filaments move around the rod circumference, helping to insert cell wall in the radial direction to reinforce rod shape. To understand how oriented MreB motion arises, we altered the shape of Bacillus subtilis. MreB motion is isotropic in round cells, and orientation is restored when rod shape is externally imposed. Stationary filaments orient within protoplasts, and purified MreB tubulates liposomes in vitro, orienting within tubes. Together, this demonstrates MreB orients along the greatest principal membrane curvature, a conclusion supported with biophysical modeling. We observed that spherical cells regenerate into rods in a local, self-reinforcing manner: rapidly propagating rods emerge from small bulges, exhibiting oriented MreB motion. We propose that the coupling of MreB filament alignment to shape-reinforcing peptidoglycan synthesis creates a locally-acting, self-organizing mechanism allowing the rapid establishment and stable maintenance of emergent rod shape.
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spelling pubmed-58544682018-03-20 MreB filaments align along greatest principal membrane curvature to orient cell wall synthesis Hussain, Saman Wivagg, Carl N Szwedziak, Piotr Wong, Felix Schaefer, Kaitlin Izoré, Thierry Renner, Lars D Holmes, Matthew J Sun, Yingjie Bisson-Filho, Alexandre W Walker, Suzanne Amir, Ariel Löwe, Jan Garner, Ethan C eLife Microbiology and Infectious Disease MreB is essential for rod shape in many bacteria. Membrane-associated MreB filaments move around the rod circumference, helping to insert cell wall in the radial direction to reinforce rod shape. To understand how oriented MreB motion arises, we altered the shape of Bacillus subtilis. MreB motion is isotropic in round cells, and orientation is restored when rod shape is externally imposed. Stationary filaments orient within protoplasts, and purified MreB tubulates liposomes in vitro, orienting within tubes. Together, this demonstrates MreB orients along the greatest principal membrane curvature, a conclusion supported with biophysical modeling. We observed that spherical cells regenerate into rods in a local, self-reinforcing manner: rapidly propagating rods emerge from small bulges, exhibiting oriented MreB motion. We propose that the coupling of MreB filament alignment to shape-reinforcing peptidoglycan synthesis creates a locally-acting, self-organizing mechanism allowing the rapid establishment and stable maintenance of emergent rod shape. eLife Sciences Publications, Ltd 2018-02-22 /pmc/articles/PMC5854468/ /pubmed/29469806 http://dx.doi.org/10.7554/eLife.32471 Text en © 2018, Hussain et al http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Microbiology and Infectious Disease
Hussain, Saman
Wivagg, Carl N
Szwedziak, Piotr
Wong, Felix
Schaefer, Kaitlin
Izoré, Thierry
Renner, Lars D
Holmes, Matthew J
Sun, Yingjie
Bisson-Filho, Alexandre W
Walker, Suzanne
Amir, Ariel
Löwe, Jan
Garner, Ethan C
MreB filaments align along greatest principal membrane curvature to orient cell wall synthesis
title MreB filaments align along greatest principal membrane curvature to orient cell wall synthesis
title_full MreB filaments align along greatest principal membrane curvature to orient cell wall synthesis
title_fullStr MreB filaments align along greatest principal membrane curvature to orient cell wall synthesis
title_full_unstemmed MreB filaments align along greatest principal membrane curvature to orient cell wall synthesis
title_short MreB filaments align along greatest principal membrane curvature to orient cell wall synthesis
title_sort mreb filaments align along greatest principal membrane curvature to orient cell wall synthesis
topic Microbiology and Infectious Disease
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5854468/
https://www.ncbi.nlm.nih.gov/pubmed/29469806
http://dx.doi.org/10.7554/eLife.32471
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