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Chromogenic detection of yam mosaic virus by closed-tube reverse transcription loop-mediated isothermal amplification (CT-RT-LAMP)

A closed-tube reverse transcription loop-mediated isothermal amplification (CT-RT-LAMP) assay was developed for the detection of yam mosaic virus (YMV, genus Potyvirus) infecting yam (Dioscorea spp.). The assay uses a set of six oligonucleotide primers targeting the YMV coat protein region, and the...

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Detalles Bibliográficos
Autores principales: Nkere, Chukwuemeka K., Oyekanmi, Joshua O., Silva, Gonçalo, Bömer, Moritz, Atiri, Gabriel I., Onyeka, Joseph, Maroya, Norbert G., Seal, Susan E., Kumar, P. Lava
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Vienna 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5854734/
https://www.ncbi.nlm.nih.gov/pubmed/29308543
http://dx.doi.org/10.1007/s00705-018-3706-0

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