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Calcium Imaging of GPCR Activation Using Arrays of Reverse Transfected HEK293 Cells in a Microfluidic System
Reverse-transfected cell arrays in microfluidic systems have great potential to perform large-scale parallel screening of G protein-coupled receptor (GPCR) activation. Here, we report the preparation of a novel platform using reverse transfection of HEK293 cells, imaging by stereo-fluorescence micro...
Autores principales: | Roelse, Margriet, Henquet, Maurice G.L., Verhoeven, Harrie A., de Ruijter, Norbert C.A., Wehrens, Ron, van Lenthe, Marco S., Witkamp, Renger F., Hall, Robert D., Jongsma, Maarten A. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5855233/ https://www.ncbi.nlm.nih.gov/pubmed/29462903 http://dx.doi.org/10.3390/s18020602 |
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