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The Protective Effect of Different Extracts of Three Artemisia Species against H(2)O(2)-Induced Oxidative Stress and Apoptosis in PC12 Neuronal Cells

BACKGROUND: Oxidative stress causes cell damage and is involved in many neurological diseases. The antioxidant properties of plant materials for the maintenance of health and protecting against different diseases stimulated scientist to investigate different herbs. Different Artemisia species have e...

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Autores principales: Hosseinzadeh, Leila, Malekshahi, Alireza, Ahmadi, Farahnaz, Emami, Seyed Ahmad, Hajialyani, Marziyeh, Mojarrab, Mahdi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medknow Publications & Media Pvt Ltd 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5855376/
https://www.ncbi.nlm.nih.gov/pubmed/29568190
http://dx.doi.org/10.4103/pr.pr_98_17
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author Hosseinzadeh, Leila
Malekshahi, Alireza
Ahmadi, Farahnaz
Emami, Seyed Ahmad
Hajialyani, Marziyeh
Mojarrab, Mahdi
author_facet Hosseinzadeh, Leila
Malekshahi, Alireza
Ahmadi, Farahnaz
Emami, Seyed Ahmad
Hajialyani, Marziyeh
Mojarrab, Mahdi
author_sort Hosseinzadeh, Leila
collection PubMed
description BACKGROUND: Oxidative stress causes cell damage and is involved in many neurological diseases. The antioxidant properties of plant materials for the maintenance of health and protecting against different diseases stimulated scientist to investigate different herbs. Different Artemisia species have exhibited antioxidant activity. This study aims to investigate whether different Artemisia species could protect the PC12 cells against oxidative stress mediated by H(2)O(2). METHODS: For this purpose, different extracts of three Artemisia species (Artemisia aucheri, Artemisia turanica, and Artemisia turcomanica) were prepared using petroleum ether, dichloromethane, ethyl acetate, ethanol, and Water: Ethanol mixture (1:1 volume ratio). The protective effect of the prepared extracts against H(2)O(2)-induced cytotoxicity and reactive oxygen species production were compared. The effect of treatment of PC12 cells with different extracts on total glutathione (GSH) level, caspase-3 activity, and mitochondrial membrane potential were also compared. RESULTS: The A. aucheri extracts could not rescue the PC12 cells from oxidative stress consequences. The A. turanica and A. turcomanica extracts were found potent in suppressing the toxicity and apoptosis of PC12 cells mediated by H(2)O(2) and significantly antagonized the H(2)O(2)-induced GSH depletion. The hydroethanolic and ethyl acetate extracts of A. turanica and the petroleum ether and hydroethanolic extracts of A. turcomanica more efficiently suppressed cytotoxicity and loss of GSH caused by H(2)O(2). CONCLUSION: This study shows the protective effects of Artemisia extracts on PC12 cell line and suggested that these species could be also considered as promising neuroprotective agents in treatment of different neurodegenerative diseases. SUMMARY: Artemisia turanica and Artemisia turcomanica extracts were found to potentially exert neuroprotective effect on PC12 cells. The results exhibited that the cytoprotective potential and anti-apoptotic mechanism of these species is not the same for different extracts, and suggested that based on the type of species and the type of solvents used in extraction, both intrinsic and extrinsic pathways could be included in the anti-apoptotic mechanism of these species. [Image: see text] Abbreviations Used: GSH: Glutathion. ROS: Reactive Oxygen Species. GSSG: Glutathione disulfide. DCF-DA:2',7'-Dichlorofluorescin diacetate. FBS: Fetal Bovin Serum. MMP: Mitochondrial Membrane Potential. H-Et: Hydro-ethanolic. DCM: Dichloromethane. PE: Petroleum Ether. Et: Etanolic. EA: Ethyl Acetate.
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spelling pubmed-58553762018-03-22 The Protective Effect of Different Extracts of Three Artemisia Species against H(2)O(2)-Induced Oxidative Stress and Apoptosis in PC12 Neuronal Cells Hosseinzadeh, Leila Malekshahi, Alireza Ahmadi, Farahnaz Emami, Seyed Ahmad Hajialyani, Marziyeh Mojarrab, Mahdi Pharmacognosy Res Original Article BACKGROUND: Oxidative stress causes cell damage and is involved in many neurological diseases. The antioxidant properties of plant materials for the maintenance of health and protecting against different diseases stimulated scientist to investigate different herbs. Different Artemisia species have exhibited antioxidant activity. This study aims to investigate whether different Artemisia species could protect the PC12 cells against oxidative stress mediated by H(2)O(2). METHODS: For this purpose, different extracts of three Artemisia species (Artemisia aucheri, Artemisia turanica, and Artemisia turcomanica) were prepared using petroleum ether, dichloromethane, ethyl acetate, ethanol, and Water: Ethanol mixture (1:1 volume ratio). The protective effect of the prepared extracts against H(2)O(2)-induced cytotoxicity and reactive oxygen species production were compared. The effect of treatment of PC12 cells with different extracts on total glutathione (GSH) level, caspase-3 activity, and mitochondrial membrane potential were also compared. RESULTS: The A. aucheri extracts could not rescue the PC12 cells from oxidative stress consequences. The A. turanica and A. turcomanica extracts were found potent in suppressing the toxicity and apoptosis of PC12 cells mediated by H(2)O(2) and significantly antagonized the H(2)O(2)-induced GSH depletion. The hydroethanolic and ethyl acetate extracts of A. turanica and the petroleum ether and hydroethanolic extracts of A. turcomanica more efficiently suppressed cytotoxicity and loss of GSH caused by H(2)O(2). CONCLUSION: This study shows the protective effects of Artemisia extracts on PC12 cell line and suggested that these species could be also considered as promising neuroprotective agents in treatment of different neurodegenerative diseases. SUMMARY: Artemisia turanica and Artemisia turcomanica extracts were found to potentially exert neuroprotective effect on PC12 cells. The results exhibited that the cytoprotective potential and anti-apoptotic mechanism of these species is not the same for different extracts, and suggested that based on the type of species and the type of solvents used in extraction, both intrinsic and extrinsic pathways could be included in the anti-apoptotic mechanism of these species. [Image: see text] Abbreviations Used: GSH: Glutathion. ROS: Reactive Oxygen Species. GSSG: Glutathione disulfide. DCF-DA:2',7'-Dichlorofluorescin diacetate. FBS: Fetal Bovin Serum. MMP: Mitochondrial Membrane Potential. H-Et: Hydro-ethanolic. DCM: Dichloromethane. PE: Petroleum Ether. Et: Etanolic. EA: Ethyl Acetate. Medknow Publications & Media Pvt Ltd 2018 /pmc/articles/PMC5855376/ /pubmed/29568190 http://dx.doi.org/10.4103/pr.pr_98_17 Text en Copyright: © 2018 Pharmacognosy Research http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 3.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as the author is credited and the new creations are licensed under the identical terms.
spellingShingle Original Article
Hosseinzadeh, Leila
Malekshahi, Alireza
Ahmadi, Farahnaz
Emami, Seyed Ahmad
Hajialyani, Marziyeh
Mojarrab, Mahdi
The Protective Effect of Different Extracts of Three Artemisia Species against H(2)O(2)-Induced Oxidative Stress and Apoptosis in PC12 Neuronal Cells
title The Protective Effect of Different Extracts of Three Artemisia Species against H(2)O(2)-Induced Oxidative Stress and Apoptosis in PC12 Neuronal Cells
title_full The Protective Effect of Different Extracts of Three Artemisia Species against H(2)O(2)-Induced Oxidative Stress and Apoptosis in PC12 Neuronal Cells
title_fullStr The Protective Effect of Different Extracts of Three Artemisia Species against H(2)O(2)-Induced Oxidative Stress and Apoptosis in PC12 Neuronal Cells
title_full_unstemmed The Protective Effect of Different Extracts of Three Artemisia Species against H(2)O(2)-Induced Oxidative Stress and Apoptosis in PC12 Neuronal Cells
title_short The Protective Effect of Different Extracts of Three Artemisia Species against H(2)O(2)-Induced Oxidative Stress and Apoptosis in PC12 Neuronal Cells
title_sort protective effect of different extracts of three artemisia species against h(2)o(2)-induced oxidative stress and apoptosis in pc12 neuronal cells
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5855376/
https://www.ncbi.nlm.nih.gov/pubmed/29568190
http://dx.doi.org/10.4103/pr.pr_98_17
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