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Biotherapeutic Effect of Gingival Stem Cells Conditioned Medium in Bone Tissue Restoration

Bone tissue engineering is one of the main branches of regenerative medicine. In this field, the use of a scaffold, which supported bone development, in combination with mesenchymal stem cells (MSCs), has promised better outcomes for bone regeneration. In particular, human gingival mesenchymal stem...

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Autores principales: Diomede, Francesca, Gugliandolo, Agnese, Scionti, Domenico, Merciaro, Ilaria, Cavalcanti, Marcos FXB, Mazzon, Emanuela, Trubiani, Oriana
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5855551/
https://www.ncbi.nlm.nih.gov/pubmed/29360771
http://dx.doi.org/10.3390/ijms19020329
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author Diomede, Francesca
Gugliandolo, Agnese
Scionti, Domenico
Merciaro, Ilaria
Cavalcanti, Marcos FXB
Mazzon, Emanuela
Trubiani, Oriana
author_facet Diomede, Francesca
Gugliandolo, Agnese
Scionti, Domenico
Merciaro, Ilaria
Cavalcanti, Marcos FXB
Mazzon, Emanuela
Trubiani, Oriana
author_sort Diomede, Francesca
collection PubMed
description Bone tissue engineering is one of the main branches of regenerative medicine. In this field, the use of a scaffold, which supported bone development, in combination with mesenchymal stem cells (MSCs), has promised better outcomes for bone regeneration. In particular, human gingival mesenchymal stem cells (hGMSCs) may present advantages compared to other MSCs, including the easier isolation. However, MSCs’ secretome has attracted much attention for its potential use in tissue regeneration, such as conditioned medium (CM) that contains different soluble factors proved to be useful for the regenerative purposes. In this study, we evaluated the osteogenic capacity of a poly-(lactide) (3D-PLA) scaffold enriched with hGMSCs and hGMSCs derived CM and its ability to regenerate bone defects in rat calvarias. 3D-PLA alone, 3D-PLA + CM or 3D-PLA + hGMSCs with/without CM were implanted in Wistar male rats subjected to calvarial defects. We observed that 3D-PLA scaffold enriched with hGMSCs and CM showed a better osteogenic capacity, being able to repair the calvarial defect as revealed in vivo by morphological evaluation. Moreover, transcriptomic analysis in vitro revealed the upregulation of genes involved in ossification and regulation of ossification in the 3D-PLA + CM + hGMSCs group. All of these results indicate the great osteogenic ability of 3D-PLA + CM + hGMSCs supporting its use in bone regenerative medicine, in particular in the repair of cranial bone defects. Especially, hGMSCs derived CM played a key role in the induction of the osteogenic process and in bone regeneration.
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spelling pubmed-58555512018-03-20 Biotherapeutic Effect of Gingival Stem Cells Conditioned Medium in Bone Tissue Restoration Diomede, Francesca Gugliandolo, Agnese Scionti, Domenico Merciaro, Ilaria Cavalcanti, Marcos FXB Mazzon, Emanuela Trubiani, Oriana Int J Mol Sci Article Bone tissue engineering is one of the main branches of regenerative medicine. In this field, the use of a scaffold, which supported bone development, in combination with mesenchymal stem cells (MSCs), has promised better outcomes for bone regeneration. In particular, human gingival mesenchymal stem cells (hGMSCs) may present advantages compared to other MSCs, including the easier isolation. However, MSCs’ secretome has attracted much attention for its potential use in tissue regeneration, such as conditioned medium (CM) that contains different soluble factors proved to be useful for the regenerative purposes. In this study, we evaluated the osteogenic capacity of a poly-(lactide) (3D-PLA) scaffold enriched with hGMSCs and hGMSCs derived CM and its ability to regenerate bone defects in rat calvarias. 3D-PLA alone, 3D-PLA + CM or 3D-PLA + hGMSCs with/without CM were implanted in Wistar male rats subjected to calvarial defects. We observed that 3D-PLA scaffold enriched with hGMSCs and CM showed a better osteogenic capacity, being able to repair the calvarial defect as revealed in vivo by morphological evaluation. Moreover, transcriptomic analysis in vitro revealed the upregulation of genes involved in ossification and regulation of ossification in the 3D-PLA + CM + hGMSCs group. All of these results indicate the great osteogenic ability of 3D-PLA + CM + hGMSCs supporting its use in bone regenerative medicine, in particular in the repair of cranial bone defects. Especially, hGMSCs derived CM played a key role in the induction of the osteogenic process and in bone regeneration. MDPI 2018-01-23 /pmc/articles/PMC5855551/ /pubmed/29360771 http://dx.doi.org/10.3390/ijms19020329 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Diomede, Francesca
Gugliandolo, Agnese
Scionti, Domenico
Merciaro, Ilaria
Cavalcanti, Marcos FXB
Mazzon, Emanuela
Trubiani, Oriana
Biotherapeutic Effect of Gingival Stem Cells Conditioned Medium in Bone Tissue Restoration
title Biotherapeutic Effect of Gingival Stem Cells Conditioned Medium in Bone Tissue Restoration
title_full Biotherapeutic Effect of Gingival Stem Cells Conditioned Medium in Bone Tissue Restoration
title_fullStr Biotherapeutic Effect of Gingival Stem Cells Conditioned Medium in Bone Tissue Restoration
title_full_unstemmed Biotherapeutic Effect of Gingival Stem Cells Conditioned Medium in Bone Tissue Restoration
title_short Biotherapeutic Effect of Gingival Stem Cells Conditioned Medium in Bone Tissue Restoration
title_sort biotherapeutic effect of gingival stem cells conditioned medium in bone tissue restoration
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5855551/
https://www.ncbi.nlm.nih.gov/pubmed/29360771
http://dx.doi.org/10.3390/ijms19020329
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