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Visualization and Quantitative 3D Analysis of Intraocular Melanoma and Its Vascularization in a Hamster Eye

A tumor vasculature network undergoes intense growth and rebuilding during tumor growth. Traditionally, vascular networks are histologically examined using parameters such as vessel density determined from two-dimensional slices of the tumor. Two-dimensional probing of a complicated three-dimensiona...

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Autores principales: Leszczyński, Bartosz, Śniegocka, Martyna, Wróbel, Andrzej, Pędrys, Roman, Szczygieł, Małgorzata, Romanowska-Dixon, Bożena, Urbańska, Krystyna, Elas, Martyna
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5855554/
https://www.ncbi.nlm.nih.gov/pubmed/29364141
http://dx.doi.org/10.3390/ijms19020332
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author Leszczyński, Bartosz
Śniegocka, Martyna
Wróbel, Andrzej
Pędrys, Roman
Szczygieł, Małgorzata
Romanowska-Dixon, Bożena
Urbańska, Krystyna
Elas, Martyna
author_facet Leszczyński, Bartosz
Śniegocka, Martyna
Wróbel, Andrzej
Pędrys, Roman
Szczygieł, Małgorzata
Romanowska-Dixon, Bożena
Urbańska, Krystyna
Elas, Martyna
author_sort Leszczyński, Bartosz
collection PubMed
description A tumor vasculature network undergoes intense growth and rebuilding during tumor growth. Traditionally, vascular networks are histologically examined using parameters such as vessel density determined from two-dimensional slices of the tumor. Two-dimensional probing of a complicated three-dimensional (3D) structure only provides partial information. Therefore, we propose the use of microcomputed tomography (micro-CT) imaging to analyze the evolution of a tumor vasculature in an experimental ocular tumor model. A Bomirski Hamster Melanoma was implanted in the anterior chamber of a hamster eye. Ultrasound (US) imaging of the same tumor was performed in vivo, and the vascular results obtained using the two methods were compared. Normal ocular tissues, a tumor, and a tumor vascular structure were revealed with high accuracy using micro-CT. The vessels that grew within the tumor were chaotic, leaky, and contained many convoluted micro-vessels and embolizations. They comprised 20–38% of the tumor mass. The blood flow in the larger functional vessels was in the range from 10 to 25 mm/s, as determined by in vivo Doppler US. The micro-CT imaging of the hamster eyeball enabled both qualitative and quantitative 3D analyses of the globe at a histological level. Although the presented images were obtained ex vivo, micro-CT noninvasive imaging is being developed intensively, and high-resolution in vivo imaging is feasible.
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spelling pubmed-58555542018-03-20 Visualization and Quantitative 3D Analysis of Intraocular Melanoma and Its Vascularization in a Hamster Eye Leszczyński, Bartosz Śniegocka, Martyna Wróbel, Andrzej Pędrys, Roman Szczygieł, Małgorzata Romanowska-Dixon, Bożena Urbańska, Krystyna Elas, Martyna Int J Mol Sci Article A tumor vasculature network undergoes intense growth and rebuilding during tumor growth. Traditionally, vascular networks are histologically examined using parameters such as vessel density determined from two-dimensional slices of the tumor. Two-dimensional probing of a complicated three-dimensional (3D) structure only provides partial information. Therefore, we propose the use of microcomputed tomography (micro-CT) imaging to analyze the evolution of a tumor vasculature in an experimental ocular tumor model. A Bomirski Hamster Melanoma was implanted in the anterior chamber of a hamster eye. Ultrasound (US) imaging of the same tumor was performed in vivo, and the vascular results obtained using the two methods were compared. Normal ocular tissues, a tumor, and a tumor vascular structure were revealed with high accuracy using micro-CT. The vessels that grew within the tumor were chaotic, leaky, and contained many convoluted micro-vessels and embolizations. They comprised 20–38% of the tumor mass. The blood flow in the larger functional vessels was in the range from 10 to 25 mm/s, as determined by in vivo Doppler US. The micro-CT imaging of the hamster eyeball enabled both qualitative and quantitative 3D analyses of the globe at a histological level. Although the presented images were obtained ex vivo, micro-CT noninvasive imaging is being developed intensively, and high-resolution in vivo imaging is feasible. MDPI 2018-01-24 /pmc/articles/PMC5855554/ /pubmed/29364141 http://dx.doi.org/10.3390/ijms19020332 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Leszczyński, Bartosz
Śniegocka, Martyna
Wróbel, Andrzej
Pędrys, Roman
Szczygieł, Małgorzata
Romanowska-Dixon, Bożena
Urbańska, Krystyna
Elas, Martyna
Visualization and Quantitative 3D Analysis of Intraocular Melanoma and Its Vascularization in a Hamster Eye
title Visualization and Quantitative 3D Analysis of Intraocular Melanoma and Its Vascularization in a Hamster Eye
title_full Visualization and Quantitative 3D Analysis of Intraocular Melanoma and Its Vascularization in a Hamster Eye
title_fullStr Visualization and Quantitative 3D Analysis of Intraocular Melanoma and Its Vascularization in a Hamster Eye
title_full_unstemmed Visualization and Quantitative 3D Analysis of Intraocular Melanoma and Its Vascularization in a Hamster Eye
title_short Visualization and Quantitative 3D Analysis of Intraocular Melanoma and Its Vascularization in a Hamster Eye
title_sort visualization and quantitative 3d analysis of intraocular melanoma and its vascularization in a hamster eye
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5855554/
https://www.ncbi.nlm.nih.gov/pubmed/29364141
http://dx.doi.org/10.3390/ijms19020332
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