Frame-Insensitive Expression Cloning of Fluorescent Protein from Scolionema suvaense

Expression cloning from cDNA is an important technique for acquiring genes encoding novel fluorescent proteins. However, the probability of in-frame cDNA insertion following the first start codon of the vector is normally only 1/3, which is a cause of low cloning efficiency. To overcome this issue,...

Descripción completa

Detalles Bibliográficos
Autores principales: Horiuchi, Yuki, Laskaratou, Danai, Sliwa, Michel, Ruckebusch, Cyril, Hatori, Kuniyuki, Mizuno, Hideaki, Hotta, Jun-ichi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5855593/
https://www.ncbi.nlm.nih.gov/pubmed/29373508
http://dx.doi.org/10.3390/ijms19020371

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5855593/
https://www.ncbi.nlm.nih.gov/pubmed/29373508
http://dx.doi.org/10.3390/ijms19020371

Ejemplares similares