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Frame-Insensitive Expression Cloning of Fluorescent Protein from Scolionema suvaense
Expression cloning from cDNA is an important technique for acquiring genes encoding novel fluorescent proteins. However, the probability of in-frame cDNA insertion following the first start codon of the vector is normally only 1/3, which is a cause of low cloning efficiency. To overcome this issue,...
| Autores principales: | , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
MDPI
2018
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5855593/ https://www.ncbi.nlm.nih.gov/pubmed/29373508 http://dx.doi.org/10.3390/ijms19020371 |