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Multifunctional Tannic Acid/Silver Nanoparticle-Based Mucoadhesive Hydrogel for Improved Local Treatment of HSV Infection: In Vitro and In Vivo Studies

Mucoadhesive gelling systems with tannic acid modified silver nanoparticles were developed for effective treatment of herpes virus infections. To increase nanoparticle residence time after local application, semi solid formulations designed from generally regarded as safe (GRAS) excipients were inve...

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Autores principales: Szymańska, Emilia, Orłowski, Piotr, Winnicka, Katarzyna, Tomaszewska, Emilia, Bąska, Piotr, Celichowski, Grzegorz, Grobelny, Jarosław, Basa, Anna, Krzyżowska, Małgorzata
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5855609/
https://www.ncbi.nlm.nih.gov/pubmed/29382085
http://dx.doi.org/10.3390/ijms19020387
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author Szymańska, Emilia
Orłowski, Piotr
Winnicka, Katarzyna
Tomaszewska, Emilia
Bąska, Piotr
Celichowski, Grzegorz
Grobelny, Jarosław
Basa, Anna
Krzyżowska, Małgorzata
author_facet Szymańska, Emilia
Orłowski, Piotr
Winnicka, Katarzyna
Tomaszewska, Emilia
Bąska, Piotr
Celichowski, Grzegorz
Grobelny, Jarosław
Basa, Anna
Krzyżowska, Małgorzata
author_sort Szymańska, Emilia
collection PubMed
description Mucoadhesive gelling systems with tannic acid modified silver nanoparticles were developed for effective treatment of herpes virus infections. To increase nanoparticle residence time after local application, semi solid formulations designed from generally regarded as safe (GRAS) excipients were investigated for their rheological and mechanical properties followed with ex vivo mucoadhesive behavior to the porcine vaginal mucosa. Particular effort was made to evaluate the activity of nanoparticle-based hydrogels toward herpes simplex virus (HSV) type 1 and 2 infection in vitro in immortal human keratinocyte cell line and in vivo using murine model of HSV-2 genital infection. The effect of infectivity was determined by real time quantitative polymerase chain reaction, plaque assay, inactivation, attachment, penetration and cell-to-cell assessments. All analyzed nanoparticle-based hydrogels exhibited pseudoplastic and thixotropic properties. Viscosity and mechanical measurements of hydrogels were found to correlate with the mucoadhesive properties. The results confirmed the ability of nanoparticle-based hydrogels to affect viral attachment, impede penetration and cell-to-cell transmission, although profound differences in the activity evoked by tested preparations toward HSV-1 and HSV-2 were noted. In addition, these findings demonstrated the in vivo potential of tannic acid modified silver nanoparticle-based hydrogels for vaginal treatment of HSV-2 genital infection.
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spelling pubmed-58556092018-03-20 Multifunctional Tannic Acid/Silver Nanoparticle-Based Mucoadhesive Hydrogel for Improved Local Treatment of HSV Infection: In Vitro and In Vivo Studies Szymańska, Emilia Orłowski, Piotr Winnicka, Katarzyna Tomaszewska, Emilia Bąska, Piotr Celichowski, Grzegorz Grobelny, Jarosław Basa, Anna Krzyżowska, Małgorzata Int J Mol Sci Article Mucoadhesive gelling systems with tannic acid modified silver nanoparticles were developed for effective treatment of herpes virus infections. To increase nanoparticle residence time after local application, semi solid formulations designed from generally regarded as safe (GRAS) excipients were investigated for their rheological and mechanical properties followed with ex vivo mucoadhesive behavior to the porcine vaginal mucosa. Particular effort was made to evaluate the activity of nanoparticle-based hydrogels toward herpes simplex virus (HSV) type 1 and 2 infection in vitro in immortal human keratinocyte cell line and in vivo using murine model of HSV-2 genital infection. The effect of infectivity was determined by real time quantitative polymerase chain reaction, plaque assay, inactivation, attachment, penetration and cell-to-cell assessments. All analyzed nanoparticle-based hydrogels exhibited pseudoplastic and thixotropic properties. Viscosity and mechanical measurements of hydrogels were found to correlate with the mucoadhesive properties. The results confirmed the ability of nanoparticle-based hydrogels to affect viral attachment, impede penetration and cell-to-cell transmission, although profound differences in the activity evoked by tested preparations toward HSV-1 and HSV-2 were noted. In addition, these findings demonstrated the in vivo potential of tannic acid modified silver nanoparticle-based hydrogels for vaginal treatment of HSV-2 genital infection. MDPI 2018-01-28 /pmc/articles/PMC5855609/ /pubmed/29382085 http://dx.doi.org/10.3390/ijms19020387 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Szymańska, Emilia
Orłowski, Piotr
Winnicka, Katarzyna
Tomaszewska, Emilia
Bąska, Piotr
Celichowski, Grzegorz
Grobelny, Jarosław
Basa, Anna
Krzyżowska, Małgorzata
Multifunctional Tannic Acid/Silver Nanoparticle-Based Mucoadhesive Hydrogel for Improved Local Treatment of HSV Infection: In Vitro and In Vivo Studies
title Multifunctional Tannic Acid/Silver Nanoparticle-Based Mucoadhesive Hydrogel for Improved Local Treatment of HSV Infection: In Vitro and In Vivo Studies
title_full Multifunctional Tannic Acid/Silver Nanoparticle-Based Mucoadhesive Hydrogel for Improved Local Treatment of HSV Infection: In Vitro and In Vivo Studies
title_fullStr Multifunctional Tannic Acid/Silver Nanoparticle-Based Mucoadhesive Hydrogel for Improved Local Treatment of HSV Infection: In Vitro and In Vivo Studies
title_full_unstemmed Multifunctional Tannic Acid/Silver Nanoparticle-Based Mucoadhesive Hydrogel for Improved Local Treatment of HSV Infection: In Vitro and In Vivo Studies
title_short Multifunctional Tannic Acid/Silver Nanoparticle-Based Mucoadhesive Hydrogel for Improved Local Treatment of HSV Infection: In Vitro and In Vivo Studies
title_sort multifunctional tannic acid/silver nanoparticle-based mucoadhesive hydrogel for improved local treatment of hsv infection: in vitro and in vivo studies
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5855609/
https://www.ncbi.nlm.nih.gov/pubmed/29382085
http://dx.doi.org/10.3390/ijms19020387
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