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Analysis of Transcriptional Responses of the Inflorescence Meristems in Jatropha curcas Following Gibberellin Treatment

Jatropha curcas L. seeds an oilseed plant with great potential for biodiesel production. However, low seed yield, which was limited by its lower female flowers, was a major drawback for its utilization. Our previous study found that the flower number and female-to-male ratio were increased by gibber...

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Autores principales: Hui, Wen-Kai, Wang, Yi, Chen, Xiao-Yang, Zayed, Mohamed Zaky, Wu, Guo-Jiang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5855654/
https://www.ncbi.nlm.nih.gov/pubmed/29389867
http://dx.doi.org/10.3390/ijms19020432
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author Hui, Wen-Kai
Wang, Yi
Chen, Xiao-Yang
Zayed, Mohamed Zaky
Wu, Guo-Jiang
author_facet Hui, Wen-Kai
Wang, Yi
Chen, Xiao-Yang
Zayed, Mohamed Zaky
Wu, Guo-Jiang
author_sort Hui, Wen-Kai
collection PubMed
description Jatropha curcas L. seeds an oilseed plant with great potential for biodiesel production. However, low seed yield, which was limited by its lower female flowers, was a major drawback for its utilization. Our previous study found that the flower number and female-to-male ratio were increased by gibberellin treatment. Here, we compared the transcriptomic profiles of inflorescence meristem at different time points after gibberellic acid A3 (GA(3)) treatment. The present study showed that 951 differentially expressed genes were obtained in response to gibberellin treatment, compared with control samples. The 6-h time point was an important phase in the response to exogenous gibberellin. Furthermore, the plant endogenous gibberellin, auxin, ethylene, abscisic acid, and brassinolide-signaling transduction pathways were repressed, whereas the genes associated with cytokinin and jasmonic acid signaling were upregulated for 24-h time point following GA(3) treatment. In addition, the floral meristem determinacy genes (JcLFY, JcSOC1) and floral organ identity genes (JcAP3, JcPI, JcSEP1-3) were significantly upregulated, but their negative regulator (JcSVP) was downregulated after GA(3) treatment. Moreover, the effects of phytohormone, which was induced by exogenous plant growth regulator, mainly acted on the female floral differentiation process. To the best of our knowledge, this data is the first comprehensive analysis of the underlying transcriptional response mechanism of floral differentiation following GA(3) treatment in J. curcas, which helps in engineering high-yielding varieties of Jatropha.
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spelling pubmed-58556542018-03-20 Analysis of Transcriptional Responses of the Inflorescence Meristems in Jatropha curcas Following Gibberellin Treatment Hui, Wen-Kai Wang, Yi Chen, Xiao-Yang Zayed, Mohamed Zaky Wu, Guo-Jiang Int J Mol Sci Article Jatropha curcas L. seeds an oilseed plant with great potential for biodiesel production. However, low seed yield, which was limited by its lower female flowers, was a major drawback for its utilization. Our previous study found that the flower number and female-to-male ratio were increased by gibberellin treatment. Here, we compared the transcriptomic profiles of inflorescence meristem at different time points after gibberellic acid A3 (GA(3)) treatment. The present study showed that 951 differentially expressed genes were obtained in response to gibberellin treatment, compared with control samples. The 6-h time point was an important phase in the response to exogenous gibberellin. Furthermore, the plant endogenous gibberellin, auxin, ethylene, abscisic acid, and brassinolide-signaling transduction pathways were repressed, whereas the genes associated with cytokinin and jasmonic acid signaling were upregulated for 24-h time point following GA(3) treatment. In addition, the floral meristem determinacy genes (JcLFY, JcSOC1) and floral organ identity genes (JcAP3, JcPI, JcSEP1-3) were significantly upregulated, but their negative regulator (JcSVP) was downregulated after GA(3) treatment. Moreover, the effects of phytohormone, which was induced by exogenous plant growth regulator, mainly acted on the female floral differentiation process. To the best of our knowledge, this data is the first comprehensive analysis of the underlying transcriptional response mechanism of floral differentiation following GA(3) treatment in J. curcas, which helps in engineering high-yielding varieties of Jatropha. MDPI 2018-02-01 /pmc/articles/PMC5855654/ /pubmed/29389867 http://dx.doi.org/10.3390/ijms19020432 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Hui, Wen-Kai
Wang, Yi
Chen, Xiao-Yang
Zayed, Mohamed Zaky
Wu, Guo-Jiang
Analysis of Transcriptional Responses of the Inflorescence Meristems in Jatropha curcas Following Gibberellin Treatment
title Analysis of Transcriptional Responses of the Inflorescence Meristems in Jatropha curcas Following Gibberellin Treatment
title_full Analysis of Transcriptional Responses of the Inflorescence Meristems in Jatropha curcas Following Gibberellin Treatment
title_fullStr Analysis of Transcriptional Responses of the Inflorescence Meristems in Jatropha curcas Following Gibberellin Treatment
title_full_unstemmed Analysis of Transcriptional Responses of the Inflorescence Meristems in Jatropha curcas Following Gibberellin Treatment
title_short Analysis of Transcriptional Responses of the Inflorescence Meristems in Jatropha curcas Following Gibberellin Treatment
title_sort analysis of transcriptional responses of the inflorescence meristems in jatropha curcas following gibberellin treatment
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5855654/
https://www.ncbi.nlm.nih.gov/pubmed/29389867
http://dx.doi.org/10.3390/ijms19020432
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