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MicroRNA-125a-5p Affects Adipocytes Proliferation, Differentiation and Fatty Acid Composition of Porcine Intramuscular Fat

Intramuscular fat (IMF) content and composition are considered crucial indicators of porcine meat quality. However, the molecular mechanism of porcine IMF development is still mostly unclear. Recently, new evidence suggested that microRNA (miRNAs) play important roles in porcine intramuscular adipog...

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Autores principales: Du, Jingjing, Xu, Yan, Zhang, Peiwen, Zhao, Xue, Gan, Mailin, Li, Qiang, Ma, Jideng, Tang, Guoqing, Jiang, Yanzhi, Wang, Jinyong, Li, Xuewei, Zhang, Shunhua, Zhu, Li
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5855723/
https://www.ncbi.nlm.nih.gov/pubmed/29414921
http://dx.doi.org/10.3390/ijms19020501
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author Du, Jingjing
Xu, Yan
Zhang, Peiwen
Zhao, Xue
Gan, Mailin
Li, Qiang
Ma, Jideng
Tang, Guoqing
Jiang, Yanzhi
Wang, Jinyong
Li, Xuewei
Zhang, Shunhua
Zhu, Li
author_facet Du, Jingjing
Xu, Yan
Zhang, Peiwen
Zhao, Xue
Gan, Mailin
Li, Qiang
Ma, Jideng
Tang, Guoqing
Jiang, Yanzhi
Wang, Jinyong
Li, Xuewei
Zhang, Shunhua
Zhu, Li
author_sort Du, Jingjing
collection PubMed
description Intramuscular fat (IMF) content and composition are considered crucial indicators of porcine meat quality. However, the molecular mechanism of porcine IMF development is still mostly unclear. Recently, new evidence suggested that microRNA (miRNAs) play important roles in porcine intramuscular adipogenesis. Previously, microRNA-125a-5p (miR-125a-5p) was identified as an important regulator of adipogenesis. In the present study, we found that the expression of miR-125a-5p is dynamically regulated during porcine intramuscular preadipocytes differentiation and that its expression levels in different porcine muscle tissues were negatively involved with IMF content. To investigate the potential function role of miR-125a-5p in IMF development, porcine intramuscular preadipocytes were collected and transfected with miR-125a-5p mimics, inhibitors, or a negative control (NC), respectively. The results showed that overexpression of miR-125a-5p promoted proliferation and inhibited differentiation of porcine intramuscular preadipocytes while inhibition of miR-125a-5p had the opposite effects. Furthermore, a luciferase reporter assay demonstrated that porcine kruppel like factor 3 (KLF13) is a target gene of miR-125a-5p during porcine intramuscular preadipocytes differentiation. Interestingly, porcine ELOVL fatty acid elongase 6 (ELOVL6), a regulator of fatty acid composition, was also identified as a target gene of miR-125a-5p during porcine intramuscular adipogenesis. Further studies show that miR-125a-5p overexpression reduced total saturated fatty acids (SFA) content and monounsaturated fatty acids (MUFA)/SFA ratios while having no significant impact on polyunsaturated fatty acids (PUFA)/SFA and n-6/n-3 ratios. Taken together, our results identified that miR-125a-5p may be a novel regulator of porcine intramuscular adipogenesis and the fatty acid composition of porcine IMF.
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spelling pubmed-58557232018-03-20 MicroRNA-125a-5p Affects Adipocytes Proliferation, Differentiation and Fatty Acid Composition of Porcine Intramuscular Fat Du, Jingjing Xu, Yan Zhang, Peiwen Zhao, Xue Gan, Mailin Li, Qiang Ma, Jideng Tang, Guoqing Jiang, Yanzhi Wang, Jinyong Li, Xuewei Zhang, Shunhua Zhu, Li Int J Mol Sci Article Intramuscular fat (IMF) content and composition are considered crucial indicators of porcine meat quality. However, the molecular mechanism of porcine IMF development is still mostly unclear. Recently, new evidence suggested that microRNA (miRNAs) play important roles in porcine intramuscular adipogenesis. Previously, microRNA-125a-5p (miR-125a-5p) was identified as an important regulator of adipogenesis. In the present study, we found that the expression of miR-125a-5p is dynamically regulated during porcine intramuscular preadipocytes differentiation and that its expression levels in different porcine muscle tissues were negatively involved with IMF content. To investigate the potential function role of miR-125a-5p in IMF development, porcine intramuscular preadipocytes were collected and transfected with miR-125a-5p mimics, inhibitors, or a negative control (NC), respectively. The results showed that overexpression of miR-125a-5p promoted proliferation and inhibited differentiation of porcine intramuscular preadipocytes while inhibition of miR-125a-5p had the opposite effects. Furthermore, a luciferase reporter assay demonstrated that porcine kruppel like factor 3 (KLF13) is a target gene of miR-125a-5p during porcine intramuscular preadipocytes differentiation. Interestingly, porcine ELOVL fatty acid elongase 6 (ELOVL6), a regulator of fatty acid composition, was also identified as a target gene of miR-125a-5p during porcine intramuscular adipogenesis. Further studies show that miR-125a-5p overexpression reduced total saturated fatty acids (SFA) content and monounsaturated fatty acids (MUFA)/SFA ratios while having no significant impact on polyunsaturated fatty acids (PUFA)/SFA and n-6/n-3 ratios. Taken together, our results identified that miR-125a-5p may be a novel regulator of porcine intramuscular adipogenesis and the fatty acid composition of porcine IMF. MDPI 2018-02-07 /pmc/articles/PMC5855723/ /pubmed/29414921 http://dx.doi.org/10.3390/ijms19020501 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Du, Jingjing
Xu, Yan
Zhang, Peiwen
Zhao, Xue
Gan, Mailin
Li, Qiang
Ma, Jideng
Tang, Guoqing
Jiang, Yanzhi
Wang, Jinyong
Li, Xuewei
Zhang, Shunhua
Zhu, Li
MicroRNA-125a-5p Affects Adipocytes Proliferation, Differentiation and Fatty Acid Composition of Porcine Intramuscular Fat
title MicroRNA-125a-5p Affects Adipocytes Proliferation, Differentiation and Fatty Acid Composition of Porcine Intramuscular Fat
title_full MicroRNA-125a-5p Affects Adipocytes Proliferation, Differentiation and Fatty Acid Composition of Porcine Intramuscular Fat
title_fullStr MicroRNA-125a-5p Affects Adipocytes Proliferation, Differentiation and Fatty Acid Composition of Porcine Intramuscular Fat
title_full_unstemmed MicroRNA-125a-5p Affects Adipocytes Proliferation, Differentiation and Fatty Acid Composition of Porcine Intramuscular Fat
title_short MicroRNA-125a-5p Affects Adipocytes Proliferation, Differentiation and Fatty Acid Composition of Porcine Intramuscular Fat
title_sort microrna-125a-5p affects adipocytes proliferation, differentiation and fatty acid composition of porcine intramuscular fat
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5855723/
https://www.ncbi.nlm.nih.gov/pubmed/29414921
http://dx.doi.org/10.3390/ijms19020501
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