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Dicer-2 promotes mRNA activation through cytoplasmic polyadenylation
Cytoplasmic polyadenylation is a widespread mechanism to regulate mRNA translation. In vertebrates, this process requires two sequence elements in target 3′ UTRs: the U-rich cytoplasmic polyadenylation element and the AAUAAA hexanucleotide. In Drosophila melanogaster, cytoplasmic polyadenylation of...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Cold Spring Harbor Laboratory Press
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5855953/ https://www.ncbi.nlm.nih.gov/pubmed/29317541 http://dx.doi.org/10.1261/rna.065417.117 |
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author | Coll, Olga Guitart, Tanit Villalba, Ana Papin, Catherine Simonelig, Martine Gebauer, Fátima |
author_facet | Coll, Olga Guitart, Tanit Villalba, Ana Papin, Catherine Simonelig, Martine Gebauer, Fátima |
author_sort | Coll, Olga |
collection | PubMed |
description | Cytoplasmic polyadenylation is a widespread mechanism to regulate mRNA translation. In vertebrates, this process requires two sequence elements in target 3′ UTRs: the U-rich cytoplasmic polyadenylation element and the AAUAAA hexanucleotide. In Drosophila melanogaster, cytoplasmic polyadenylation of Toll mRNA occurs independently of these canonical elements and requires a machinery that remains to be characterized. Here we identify Dicer-2 as a component of this machinery. Dicer-2, a factor previously involved in RNA interference (RNAi), interacts with the cytoplasmic poly(A) polymerase Wispy. Depletion of Dicer-2 from polyadenylation-competent embryo extracts and analysis of wispy mutants indicate that both factors are necessary for polyadenylation and translation of Toll mRNA. We further identify r2d2 mRNA, encoding a Dicer-2 partner in RNAi, as a Dicer-2 polyadenylation target. Our results uncover a novel function of Dicer-2 in activation of mRNA translation through cytoplasmic polyadenylation. |
format | Online Article Text |
id | pubmed-5855953 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Cold Spring Harbor Laboratory Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-58559532018-04-01 Dicer-2 promotes mRNA activation through cytoplasmic polyadenylation Coll, Olga Guitart, Tanit Villalba, Ana Papin, Catherine Simonelig, Martine Gebauer, Fátima RNA Article Cytoplasmic polyadenylation is a widespread mechanism to regulate mRNA translation. In vertebrates, this process requires two sequence elements in target 3′ UTRs: the U-rich cytoplasmic polyadenylation element and the AAUAAA hexanucleotide. In Drosophila melanogaster, cytoplasmic polyadenylation of Toll mRNA occurs independently of these canonical elements and requires a machinery that remains to be characterized. Here we identify Dicer-2 as a component of this machinery. Dicer-2, a factor previously involved in RNA interference (RNAi), interacts with the cytoplasmic poly(A) polymerase Wispy. Depletion of Dicer-2 from polyadenylation-competent embryo extracts and analysis of wispy mutants indicate that both factors are necessary for polyadenylation and translation of Toll mRNA. We further identify r2d2 mRNA, encoding a Dicer-2 partner in RNAi, as a Dicer-2 polyadenylation target. Our results uncover a novel function of Dicer-2 in activation of mRNA translation through cytoplasmic polyadenylation. Cold Spring Harbor Laboratory Press 2018-04 /pmc/articles/PMC5855953/ /pubmed/29317541 http://dx.doi.org/10.1261/rna.065417.117 Text en © 2018 Coll et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society http://creativecommons.org/licenses/by/4.0/ This article, published in RNA, is available under a Creative Commons License (Attribution 4.0 International), as described at http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Coll, Olga Guitart, Tanit Villalba, Ana Papin, Catherine Simonelig, Martine Gebauer, Fátima Dicer-2 promotes mRNA activation through cytoplasmic polyadenylation |
title | Dicer-2 promotes mRNA activation through cytoplasmic polyadenylation |
title_full | Dicer-2 promotes mRNA activation through cytoplasmic polyadenylation |
title_fullStr | Dicer-2 promotes mRNA activation through cytoplasmic polyadenylation |
title_full_unstemmed | Dicer-2 promotes mRNA activation through cytoplasmic polyadenylation |
title_short | Dicer-2 promotes mRNA activation through cytoplasmic polyadenylation |
title_sort | dicer-2 promotes mrna activation through cytoplasmic polyadenylation |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5855953/ https://www.ncbi.nlm.nih.gov/pubmed/29317541 http://dx.doi.org/10.1261/rna.065417.117 |
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