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Dicer-2 promotes mRNA activation through cytoplasmic polyadenylation

Cytoplasmic polyadenylation is a widespread mechanism to regulate mRNA translation. In vertebrates, this process requires two sequence elements in target 3′ UTRs: the U-rich cytoplasmic polyadenylation element and the AAUAAA hexanucleotide. In Drosophila melanogaster, cytoplasmic polyadenylation of...

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Autores principales: Coll, Olga, Guitart, Tanit, Villalba, Ana, Papin, Catherine, Simonelig, Martine, Gebauer, Fátima
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory Press 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5855953/
https://www.ncbi.nlm.nih.gov/pubmed/29317541
http://dx.doi.org/10.1261/rna.065417.117
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author Coll, Olga
Guitart, Tanit
Villalba, Ana
Papin, Catherine
Simonelig, Martine
Gebauer, Fátima
author_facet Coll, Olga
Guitart, Tanit
Villalba, Ana
Papin, Catherine
Simonelig, Martine
Gebauer, Fátima
author_sort Coll, Olga
collection PubMed
description Cytoplasmic polyadenylation is a widespread mechanism to regulate mRNA translation. In vertebrates, this process requires two sequence elements in target 3′ UTRs: the U-rich cytoplasmic polyadenylation element and the AAUAAA hexanucleotide. In Drosophila melanogaster, cytoplasmic polyadenylation of Toll mRNA occurs independently of these canonical elements and requires a machinery that remains to be characterized. Here we identify Dicer-2 as a component of this machinery. Dicer-2, a factor previously involved in RNA interference (RNAi), interacts with the cytoplasmic poly(A) polymerase Wispy. Depletion of Dicer-2 from polyadenylation-competent embryo extracts and analysis of wispy mutants indicate that both factors are necessary for polyadenylation and translation of Toll mRNA. We further identify r2d2 mRNA, encoding a Dicer-2 partner in RNAi, as a Dicer-2 polyadenylation target. Our results uncover a novel function of Dicer-2 in activation of mRNA translation through cytoplasmic polyadenylation.
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spelling pubmed-58559532018-04-01 Dicer-2 promotes mRNA activation through cytoplasmic polyadenylation Coll, Olga Guitart, Tanit Villalba, Ana Papin, Catherine Simonelig, Martine Gebauer, Fátima RNA Article Cytoplasmic polyadenylation is a widespread mechanism to regulate mRNA translation. In vertebrates, this process requires two sequence elements in target 3′ UTRs: the U-rich cytoplasmic polyadenylation element and the AAUAAA hexanucleotide. In Drosophila melanogaster, cytoplasmic polyadenylation of Toll mRNA occurs independently of these canonical elements and requires a machinery that remains to be characterized. Here we identify Dicer-2 as a component of this machinery. Dicer-2, a factor previously involved in RNA interference (RNAi), interacts with the cytoplasmic poly(A) polymerase Wispy. Depletion of Dicer-2 from polyadenylation-competent embryo extracts and analysis of wispy mutants indicate that both factors are necessary for polyadenylation and translation of Toll mRNA. We further identify r2d2 mRNA, encoding a Dicer-2 partner in RNAi, as a Dicer-2 polyadenylation target. Our results uncover a novel function of Dicer-2 in activation of mRNA translation through cytoplasmic polyadenylation. Cold Spring Harbor Laboratory Press 2018-04 /pmc/articles/PMC5855953/ /pubmed/29317541 http://dx.doi.org/10.1261/rna.065417.117 Text en © 2018 Coll et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society http://creativecommons.org/licenses/by/4.0/ This article, published in RNA, is available under a Creative Commons License (Attribution 4.0 International), as described at http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Coll, Olga
Guitart, Tanit
Villalba, Ana
Papin, Catherine
Simonelig, Martine
Gebauer, Fátima
Dicer-2 promotes mRNA activation through cytoplasmic polyadenylation
title Dicer-2 promotes mRNA activation through cytoplasmic polyadenylation
title_full Dicer-2 promotes mRNA activation through cytoplasmic polyadenylation
title_fullStr Dicer-2 promotes mRNA activation through cytoplasmic polyadenylation
title_full_unstemmed Dicer-2 promotes mRNA activation through cytoplasmic polyadenylation
title_short Dicer-2 promotes mRNA activation through cytoplasmic polyadenylation
title_sort dicer-2 promotes mrna activation through cytoplasmic polyadenylation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5855953/
https://www.ncbi.nlm.nih.gov/pubmed/29317541
http://dx.doi.org/10.1261/rna.065417.117
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