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Electrochemical Immunoassay Using Open Circuit Potential Detection Labeled by Platinum Nanoparticles
In this work, a simple electrochemical immunoassay based on platinum nanoparticles (PtNPs) using open circuit potential (OCP) detection was developed. The detection of human chorionic gonadotropin hormone (hCG) as a model analyte, was demonstrated by direct electrical detection of PtNPs in hydrazine...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5855978/ https://www.ncbi.nlm.nih.gov/pubmed/29401642 http://dx.doi.org/10.3390/s18020444 |
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author | Charoenkitamorn, Kanokwan Tue, Phan Trong Kawai, Keiko Chailapakul, Orawon Takamura, Yuzuru |
author_facet | Charoenkitamorn, Kanokwan Tue, Phan Trong Kawai, Keiko Chailapakul, Orawon Takamura, Yuzuru |
author_sort | Charoenkitamorn, Kanokwan |
collection | PubMed |
description | In this work, a simple electrochemical immunoassay based on platinum nanoparticles (PtNPs) using open circuit potential (OCP) detection was developed. The detection of human chorionic gonadotropin hormone (hCG) as a model analyte, was demonstrated by direct electrical detection of PtNPs in hydrazine solution using OCP measurement without any application of either potential or current to the system. Disposable screen-printed carbon electrodes (SPCEs) were utilized for the development of our immunosensor, which required a sample volume as small as 2 μL. After preparation of a sandwich-type immunosystem, hydrazine solution was dropped on the electrode’s surface, which was followed immediately by electrical detection using OCP. The change of the OCP signal originated from electrocatalytic oxidation of the hydrazine on PtNPs. Under the optimal conditions of a pH of 6.0 and a hydrazine concentration of 1 mM, a detection limit of 0.28 ng mL(−1) and a linearity of 0–10 ng mL(−1) were obtained. The PtNP-based OCP method is a simpler electrochemical detection procedure than those obtained from other electrochemical methods and has an acceptable sensitivity and reproducibility. The simplicity of the detection procedure and the cost-effectiveness of the disposable SPCE illustrate the attractive benefits of this sensor. Moreover, it could be applied to a simplified and miniaturized diagnostic system with minimal user manipulation. |
format | Online Article Text |
id | pubmed-5855978 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-58559782018-03-20 Electrochemical Immunoassay Using Open Circuit Potential Detection Labeled by Platinum Nanoparticles Charoenkitamorn, Kanokwan Tue, Phan Trong Kawai, Keiko Chailapakul, Orawon Takamura, Yuzuru Sensors (Basel) Article In this work, a simple electrochemical immunoassay based on platinum nanoparticles (PtNPs) using open circuit potential (OCP) detection was developed. The detection of human chorionic gonadotropin hormone (hCG) as a model analyte, was demonstrated by direct electrical detection of PtNPs in hydrazine solution using OCP measurement without any application of either potential or current to the system. Disposable screen-printed carbon electrodes (SPCEs) were utilized for the development of our immunosensor, which required a sample volume as small as 2 μL. After preparation of a sandwich-type immunosystem, hydrazine solution was dropped on the electrode’s surface, which was followed immediately by electrical detection using OCP. The change of the OCP signal originated from electrocatalytic oxidation of the hydrazine on PtNPs. Under the optimal conditions of a pH of 6.0 and a hydrazine concentration of 1 mM, a detection limit of 0.28 ng mL(−1) and a linearity of 0–10 ng mL(−1) were obtained. The PtNP-based OCP method is a simpler electrochemical detection procedure than those obtained from other electrochemical methods and has an acceptable sensitivity and reproducibility. The simplicity of the detection procedure and the cost-effectiveness of the disposable SPCE illustrate the attractive benefits of this sensor. Moreover, it could be applied to a simplified and miniaturized diagnostic system with minimal user manipulation. MDPI 2018-02-03 /pmc/articles/PMC5855978/ /pubmed/29401642 http://dx.doi.org/10.3390/s18020444 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Charoenkitamorn, Kanokwan Tue, Phan Trong Kawai, Keiko Chailapakul, Orawon Takamura, Yuzuru Electrochemical Immunoassay Using Open Circuit Potential Detection Labeled by Platinum Nanoparticles |
title | Electrochemical Immunoassay Using Open Circuit Potential Detection Labeled by Platinum Nanoparticles |
title_full | Electrochemical Immunoassay Using Open Circuit Potential Detection Labeled by Platinum Nanoparticles |
title_fullStr | Electrochemical Immunoassay Using Open Circuit Potential Detection Labeled by Platinum Nanoparticles |
title_full_unstemmed | Electrochemical Immunoassay Using Open Circuit Potential Detection Labeled by Platinum Nanoparticles |
title_short | Electrochemical Immunoassay Using Open Circuit Potential Detection Labeled by Platinum Nanoparticles |
title_sort | electrochemical immunoassay using open circuit potential detection labeled by platinum nanoparticles |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5855978/ https://www.ncbi.nlm.nih.gov/pubmed/29401642 http://dx.doi.org/10.3390/s18020444 |
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