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Carriage of colistin-resistant, extended-spectrum β-lactamase-producing Escherichia coli harboring the mcr-1 resistance gene after short-term international travel to Vietnam

BACKGROUND: Due to increasing colistin usage, the dissemination of the colistin-resistant gene mcr-1 has been increasingly investigated. The aim of this study was to determine whether a traveler on a short-term international trip to a developing country could bring mcr-1 back to their home country....

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Autores principales: Nakayama, Tatsuya, Kumeda, Yuko, Kawahara, Ryuji, Yamaguchi, Takahiro, Yamamoto, Yoshimasa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove Medical Press 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5856025/
https://www.ncbi.nlm.nih.gov/pubmed/29559800
http://dx.doi.org/10.2147/IDR.S153178
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author Nakayama, Tatsuya
Kumeda, Yuko
Kawahara, Ryuji
Yamaguchi, Takahiro
Yamamoto, Yoshimasa
author_facet Nakayama, Tatsuya
Kumeda, Yuko
Kawahara, Ryuji
Yamaguchi, Takahiro
Yamamoto, Yoshimasa
author_sort Nakayama, Tatsuya
collection PubMed
description BACKGROUND: Due to increasing colistin usage, the dissemination of the colistin-resistant gene mcr-1 has been increasingly investigated. The aim of this study was to determine whether a traveler on a short-term international trip to a developing country could bring mcr-1 back to their home country. MATERIALS AND METHODS: Thirty-four travel events from Japan to Vietnam encompassing 19 travelers were assessed. A fecal specimen was collected from each traveler before and after each travel event and was inoculated on CHROMagar containing cefotaxime (CTX). Three to seven colonies exhibiting the characteristics of Escherichia coli were collected. Susceptibility to antibiotics and extended-spectrum β-lactamase (ESBL) production were determined by the disk diffusion method and the double-disk synergy test, respectively. ESBL-encoding genes were genotyped, and phylogenetic groupings were determined by multiplex polymerase chain reaction (PCR). The presence of mcr-1 was also confirmed by PCR and sequencing. RESULTS: A total of 175 ESBL-producing E. coli isolated before and up to 2 weeks after traveling to Vietnam were analyzed. Genotyping of ESBL-producing isolates showed that bla(CTX-M-1)/bla(TEM) (27.7%) and bla(CTX-M-9) (45.9%) were the most prevalent genotypes, while the most frequently detected phylogenetic group was D (41.9%) followed by B2 (23.0%). In a significant number of travel events, travelers brought ESBL-producing E. coli back to Japan and three events by three travelers carried mcr-1. ESBL-producing E. coli isolates harboring mcr-1 were identified as those carrying both bla(CTX-M-14) or bla(CTX-M-55) and mcr-1. CONCLUSION: Using Vietnam as an example, we have shown that even a short-term trip to some countries may result in ESBL-producing mcr-1-positive E. coli carriage by international travelers.
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spelling pubmed-58560252018-03-20 Carriage of colistin-resistant, extended-spectrum β-lactamase-producing Escherichia coli harboring the mcr-1 resistance gene after short-term international travel to Vietnam Nakayama, Tatsuya Kumeda, Yuko Kawahara, Ryuji Yamaguchi, Takahiro Yamamoto, Yoshimasa Infect Drug Resist Original Research BACKGROUND: Due to increasing colistin usage, the dissemination of the colistin-resistant gene mcr-1 has been increasingly investigated. The aim of this study was to determine whether a traveler on a short-term international trip to a developing country could bring mcr-1 back to their home country. MATERIALS AND METHODS: Thirty-four travel events from Japan to Vietnam encompassing 19 travelers were assessed. A fecal specimen was collected from each traveler before and after each travel event and was inoculated on CHROMagar containing cefotaxime (CTX). Three to seven colonies exhibiting the characteristics of Escherichia coli were collected. Susceptibility to antibiotics and extended-spectrum β-lactamase (ESBL) production were determined by the disk diffusion method and the double-disk synergy test, respectively. ESBL-encoding genes were genotyped, and phylogenetic groupings were determined by multiplex polymerase chain reaction (PCR). The presence of mcr-1 was also confirmed by PCR and sequencing. RESULTS: A total of 175 ESBL-producing E. coli isolated before and up to 2 weeks after traveling to Vietnam were analyzed. Genotyping of ESBL-producing isolates showed that bla(CTX-M-1)/bla(TEM) (27.7%) and bla(CTX-M-9) (45.9%) were the most prevalent genotypes, while the most frequently detected phylogenetic group was D (41.9%) followed by B2 (23.0%). In a significant number of travel events, travelers brought ESBL-producing E. coli back to Japan and three events by three travelers carried mcr-1. ESBL-producing E. coli isolates harboring mcr-1 were identified as those carrying both bla(CTX-M-14) or bla(CTX-M-55) and mcr-1. CONCLUSION: Using Vietnam as an example, we have shown that even a short-term trip to some countries may result in ESBL-producing mcr-1-positive E. coli carriage by international travelers. Dove Medical Press 2018-03-12 /pmc/articles/PMC5856025/ /pubmed/29559800 http://dx.doi.org/10.2147/IDR.S153178 Text en © 2018 Nakayama et al. This work is published and licensed by Dove Medical Press Limited The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed.
spellingShingle Original Research
Nakayama, Tatsuya
Kumeda, Yuko
Kawahara, Ryuji
Yamaguchi, Takahiro
Yamamoto, Yoshimasa
Carriage of colistin-resistant, extended-spectrum β-lactamase-producing Escherichia coli harboring the mcr-1 resistance gene after short-term international travel to Vietnam
title Carriage of colistin-resistant, extended-spectrum β-lactamase-producing Escherichia coli harboring the mcr-1 resistance gene after short-term international travel to Vietnam
title_full Carriage of colistin-resistant, extended-spectrum β-lactamase-producing Escherichia coli harboring the mcr-1 resistance gene after short-term international travel to Vietnam
title_fullStr Carriage of colistin-resistant, extended-spectrum β-lactamase-producing Escherichia coli harboring the mcr-1 resistance gene after short-term international travel to Vietnam
title_full_unstemmed Carriage of colistin-resistant, extended-spectrum β-lactamase-producing Escherichia coli harboring the mcr-1 resistance gene after short-term international travel to Vietnam
title_short Carriage of colistin-resistant, extended-spectrum β-lactamase-producing Escherichia coli harboring the mcr-1 resistance gene after short-term international travel to Vietnam
title_sort carriage of colistin-resistant, extended-spectrum β-lactamase-producing escherichia coli harboring the mcr-1 resistance gene after short-term international travel to vietnam
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5856025/
https://www.ncbi.nlm.nih.gov/pubmed/29559800
http://dx.doi.org/10.2147/IDR.S153178
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