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Intermittent PTH administration improves alveolar bone formation in type 1 diabetic rats with periodontitis
BACKGROUND: Periodontitis is an infectious disease that manifests as alveolar bone loss surrounding the roots of teeth. Diabetes aggravates periodontitis-induced alveolar bone loss via suppression of bone formation. Intermittent parathyroid hormone (PTH) administration displays an anabolic effect on...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5856320/ https://www.ncbi.nlm.nih.gov/pubmed/29544500 http://dx.doi.org/10.1186/s12967-018-1438-2 |
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author | Kim, Ji-Hye Kim, Ae Ri Choi, Yun Hui Kim, Aeryun Sohn, Yongsung Woo, Gye-Hyeong Cha, Jeong-Heon Bak, Eun-Jung Yoo, Yun-Jung |
author_facet | Kim, Ji-Hye Kim, Ae Ri Choi, Yun Hui Kim, Aeryun Sohn, Yongsung Woo, Gye-Hyeong Cha, Jeong-Heon Bak, Eun-Jung Yoo, Yun-Jung |
author_sort | Kim, Ji-Hye |
collection | PubMed |
description | BACKGROUND: Periodontitis is an infectious disease that manifests as alveolar bone loss surrounding the roots of teeth. Diabetes aggravates periodontitis-induced alveolar bone loss via suppression of bone formation. Intermittent parathyroid hormone (PTH) administration displays an anabolic effect on bone. In this study, we investigated the effect of intermittent PTH administration on alveolar bone loss in type 1 diabetic rats with periodontitis. METHODS: Rats were divided into control (C), periodontitis (P), periodontitis treated with PTH (P + PTH), diabetes with periodontitis (DP), and diabetes with periodontitis treated with PTH (DP + PTH) groups. To induce type 1 diabetes, rats were injected with streptozotocin and periodontitis was induced bilaterally by applying ligatures to the mandibular first molars for 30 days. During the experimental period, the P + PTH and DP + PTH groups were subcutaneously injected with PTH (40 μg/kg) three times per week, whereas the C, P, and DP groups were injected with citrate buffer. To observe the mineralization of the alveolar bone, the DP and DP + PTH groups were injected with calcein on days 10 and 27, and with alizarin red on day 20. Thirty days after ligation, histological findings and fluorescence labeling were analyzed in the furcations of the mandibular first molars. Sclerostin-positive osteocytes were assessed by immunohistochemical analyses. RESULTS: The DP groups had smaller areas of alveolar bone than the other groups, and the DP + PTH group had a larger alveolar bone area than the DP group. The DP group had less osteoid formation than the C group, whereas the DP + PTH had greater osteoid formation than the DP group. Fluorescence labeling results revealed that the DP + PTH group had more mineral deposition on the alveolar bone than the DP group. The DP + PTH group exhibited lower percentage of sclerostin-positive osteocytes in alveolar bone than the DP group. CONCLUSIONS: Intermittent PTH administration diminishes alveolar bone loss and sclerostin expression in osteocytes, but increases osteoid formation and mineralization, suggesting that intermittent PTH administration attenuates diabetes-aggravated alveolar bone loss by the induction of bone formation. PTH-induced bone formation may be related to the regulation of osteocytic sclerostin expression in type 1 diabetic rats with periodontitis. |
format | Online Article Text |
id | pubmed-5856320 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-58563202018-03-22 Intermittent PTH administration improves alveolar bone formation in type 1 diabetic rats with periodontitis Kim, Ji-Hye Kim, Ae Ri Choi, Yun Hui Kim, Aeryun Sohn, Yongsung Woo, Gye-Hyeong Cha, Jeong-Heon Bak, Eun-Jung Yoo, Yun-Jung J Transl Med Research BACKGROUND: Periodontitis is an infectious disease that manifests as alveolar bone loss surrounding the roots of teeth. Diabetes aggravates periodontitis-induced alveolar bone loss via suppression of bone formation. Intermittent parathyroid hormone (PTH) administration displays an anabolic effect on bone. In this study, we investigated the effect of intermittent PTH administration on alveolar bone loss in type 1 diabetic rats with periodontitis. METHODS: Rats were divided into control (C), periodontitis (P), periodontitis treated with PTH (P + PTH), diabetes with periodontitis (DP), and diabetes with periodontitis treated with PTH (DP + PTH) groups. To induce type 1 diabetes, rats were injected with streptozotocin and periodontitis was induced bilaterally by applying ligatures to the mandibular first molars for 30 days. During the experimental period, the P + PTH and DP + PTH groups were subcutaneously injected with PTH (40 μg/kg) three times per week, whereas the C, P, and DP groups were injected with citrate buffer. To observe the mineralization of the alveolar bone, the DP and DP + PTH groups were injected with calcein on days 10 and 27, and with alizarin red on day 20. Thirty days after ligation, histological findings and fluorescence labeling were analyzed in the furcations of the mandibular first molars. Sclerostin-positive osteocytes were assessed by immunohistochemical analyses. RESULTS: The DP groups had smaller areas of alveolar bone than the other groups, and the DP + PTH group had a larger alveolar bone area than the DP group. The DP group had less osteoid formation than the C group, whereas the DP + PTH had greater osteoid formation than the DP group. Fluorescence labeling results revealed that the DP + PTH group had more mineral deposition on the alveolar bone than the DP group. The DP + PTH group exhibited lower percentage of sclerostin-positive osteocytes in alveolar bone than the DP group. CONCLUSIONS: Intermittent PTH administration diminishes alveolar bone loss and sclerostin expression in osteocytes, but increases osteoid formation and mineralization, suggesting that intermittent PTH administration attenuates diabetes-aggravated alveolar bone loss by the induction of bone formation. PTH-induced bone formation may be related to the regulation of osteocytic sclerostin expression in type 1 diabetic rats with periodontitis. BioMed Central 2018-03-15 /pmc/articles/PMC5856320/ /pubmed/29544500 http://dx.doi.org/10.1186/s12967-018-1438-2 Text en © The Author(s) 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Kim, Ji-Hye Kim, Ae Ri Choi, Yun Hui Kim, Aeryun Sohn, Yongsung Woo, Gye-Hyeong Cha, Jeong-Heon Bak, Eun-Jung Yoo, Yun-Jung Intermittent PTH administration improves alveolar bone formation in type 1 diabetic rats with periodontitis |
title | Intermittent PTH administration improves alveolar bone formation in type 1 diabetic rats with periodontitis |
title_full | Intermittent PTH administration improves alveolar bone formation in type 1 diabetic rats with periodontitis |
title_fullStr | Intermittent PTH administration improves alveolar bone formation in type 1 diabetic rats with periodontitis |
title_full_unstemmed | Intermittent PTH administration improves alveolar bone formation in type 1 diabetic rats with periodontitis |
title_short | Intermittent PTH administration improves alveolar bone formation in type 1 diabetic rats with periodontitis |
title_sort | intermittent pth administration improves alveolar bone formation in type 1 diabetic rats with periodontitis |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5856320/ https://www.ncbi.nlm.nih.gov/pubmed/29544500 http://dx.doi.org/10.1186/s12967-018-1438-2 |
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