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Functional architecture of reward learning in mushroom body extrinsic neurons of larval Drosophila

The brain adaptively integrates present sensory input, past experience, and options for future action. The insect mushroom body exemplifies how a central brain structure brings about such integration. Here we use a combination of systematic single-cell labeling, connectomics, transgenic silencing, a...

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Autores principales: Saumweber, Timo, Rohwedder, Astrid, Schleyer, Michael, Eichler, Katharina, Chen, Yi-chun, Aso, Yoshinori, Cardona, Albert, Eschbach, Claire, Kobler, Oliver, Voigt, Anne, Durairaja, Archana, Mancini, Nino, Zlatic, Marta, Truman, James W., Thum, Andreas S., Gerber, Bertram
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5856778/
https://www.ncbi.nlm.nih.gov/pubmed/29549237
http://dx.doi.org/10.1038/s41467-018-03130-1
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author Saumweber, Timo
Rohwedder, Astrid
Schleyer, Michael
Eichler, Katharina
Chen, Yi-chun
Aso, Yoshinori
Cardona, Albert
Eschbach, Claire
Kobler, Oliver
Voigt, Anne
Durairaja, Archana
Mancini, Nino
Zlatic, Marta
Truman, James W.
Thum, Andreas S.
Gerber, Bertram
author_facet Saumweber, Timo
Rohwedder, Astrid
Schleyer, Michael
Eichler, Katharina
Chen, Yi-chun
Aso, Yoshinori
Cardona, Albert
Eschbach, Claire
Kobler, Oliver
Voigt, Anne
Durairaja, Archana
Mancini, Nino
Zlatic, Marta
Truman, James W.
Thum, Andreas S.
Gerber, Bertram
author_sort Saumweber, Timo
collection PubMed
description The brain adaptively integrates present sensory input, past experience, and options for future action. The insect mushroom body exemplifies how a central brain structure brings about such integration. Here we use a combination of systematic single-cell labeling, connectomics, transgenic silencing, and activation experiments to study the mushroom body at single-cell resolution, focusing on the behavioral architecture of its input and output neurons (MBINs and MBONs), and of the mushroom body intrinsic APL neuron. Our results reveal the identity and morphology of almost all of these 44 neurons in stage 3 Drosophila larvae. Upon an initial screen, functional analyses focusing on the mushroom body medial lobe uncover sparse and specific functions of its dopaminergic MBINs, its MBONs, and of the GABAergic APL neuron across three behavioral tasks, namely odor preference, taste preference, and associative learning between odor and taste. Our results thus provide a cellular-resolution study case of how brains organize behavior.
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spelling pubmed-58567782018-03-20 Functional architecture of reward learning in mushroom body extrinsic neurons of larval Drosophila Saumweber, Timo Rohwedder, Astrid Schleyer, Michael Eichler, Katharina Chen, Yi-chun Aso, Yoshinori Cardona, Albert Eschbach, Claire Kobler, Oliver Voigt, Anne Durairaja, Archana Mancini, Nino Zlatic, Marta Truman, James W. Thum, Andreas S. Gerber, Bertram Nat Commun Article The brain adaptively integrates present sensory input, past experience, and options for future action. The insect mushroom body exemplifies how a central brain structure brings about such integration. Here we use a combination of systematic single-cell labeling, connectomics, transgenic silencing, and activation experiments to study the mushroom body at single-cell resolution, focusing on the behavioral architecture of its input and output neurons (MBINs and MBONs), and of the mushroom body intrinsic APL neuron. Our results reveal the identity and morphology of almost all of these 44 neurons in stage 3 Drosophila larvae. Upon an initial screen, functional analyses focusing on the mushroom body medial lobe uncover sparse and specific functions of its dopaminergic MBINs, its MBONs, and of the GABAergic APL neuron across three behavioral tasks, namely odor preference, taste preference, and associative learning between odor and taste. Our results thus provide a cellular-resolution study case of how brains organize behavior. Nature Publishing Group UK 2018-03-16 /pmc/articles/PMC5856778/ /pubmed/29549237 http://dx.doi.org/10.1038/s41467-018-03130-1 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Saumweber, Timo
Rohwedder, Astrid
Schleyer, Michael
Eichler, Katharina
Chen, Yi-chun
Aso, Yoshinori
Cardona, Albert
Eschbach, Claire
Kobler, Oliver
Voigt, Anne
Durairaja, Archana
Mancini, Nino
Zlatic, Marta
Truman, James W.
Thum, Andreas S.
Gerber, Bertram
Functional architecture of reward learning in mushroom body extrinsic neurons of larval Drosophila
title Functional architecture of reward learning in mushroom body extrinsic neurons of larval Drosophila
title_full Functional architecture of reward learning in mushroom body extrinsic neurons of larval Drosophila
title_fullStr Functional architecture of reward learning in mushroom body extrinsic neurons of larval Drosophila
title_full_unstemmed Functional architecture of reward learning in mushroom body extrinsic neurons of larval Drosophila
title_short Functional architecture of reward learning in mushroom body extrinsic neurons of larval Drosophila
title_sort functional architecture of reward learning in mushroom body extrinsic neurons of larval drosophila
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5856778/
https://www.ncbi.nlm.nih.gov/pubmed/29549237
http://dx.doi.org/10.1038/s41467-018-03130-1
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