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Downregulation of lumican accelerates lung cancer cell invasion through p120 catenin
The overexpression of lumican has been found in lung cancer cells; however, the functional role of lumican in lung cancer cells remains unclear. In this study, we found lumican functioned as a tubulin-binding protein and the depletion of lumican by transfection with its specific shRNA increased lung...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5856799/ https://www.ncbi.nlm.nih.gov/pubmed/29549325 http://dx.doi.org/10.1038/s41419-017-0212-3 |
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author | Yang, Cheng-Ta Li, Jhy-Ming Chu, Wing-Keung Chow, Shu-Er |
author_facet | Yang, Cheng-Ta Li, Jhy-Ming Chu, Wing-Keung Chow, Shu-Er |
author_sort | Yang, Cheng-Ta |
collection | PubMed |
description | The overexpression of lumican has been found in lung cancer cells; however, the functional role of lumican in lung cancer cells remains unclear. In this study, we found lumican functioned as a tubulin-binding protein and the depletion of lumican by transfection with its specific shRNA increased lung cancer cell invasion. Such alterations led to morphological changes and actin cytoskeleton remodeling, including the induction of membrane ruffling or protrusion and stress fiber formation, correlated with the increased activities of Rac and Rho. The downregulation of lumican was also implicated in macrophage-conditioned media (maCM)-induced cell invasion. Immunofluorescence images and immunoprecipitation assays revealed the co-localization of p120-catenin (p120ctn) and lumican. Reduction in the levels of p120ctn induced membrane ruffling and the activation of the Rho family, which accelerated cell invasion. Our data indicated that lumican is associated with microtubule-modulated p120ctn signaling, providing important insights into lung cancer progression. |
format | Online Article Text |
id | pubmed-5856799 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-58567992018-05-31 Downregulation of lumican accelerates lung cancer cell invasion through p120 catenin Yang, Cheng-Ta Li, Jhy-Ming Chu, Wing-Keung Chow, Shu-Er Cell Death Dis Article The overexpression of lumican has been found in lung cancer cells; however, the functional role of lumican in lung cancer cells remains unclear. In this study, we found lumican functioned as a tubulin-binding protein and the depletion of lumican by transfection with its specific shRNA increased lung cancer cell invasion. Such alterations led to morphological changes and actin cytoskeleton remodeling, including the induction of membrane ruffling or protrusion and stress fiber formation, correlated with the increased activities of Rac and Rho. The downregulation of lumican was also implicated in macrophage-conditioned media (maCM)-induced cell invasion. Immunofluorescence images and immunoprecipitation assays revealed the co-localization of p120-catenin (p120ctn) and lumican. Reduction in the levels of p120ctn induced membrane ruffling and the activation of the Rho family, which accelerated cell invasion. Our data indicated that lumican is associated with microtubule-modulated p120ctn signaling, providing important insights into lung cancer progression. Nature Publishing Group UK 2018-03-16 /pmc/articles/PMC5856799/ /pubmed/29549325 http://dx.doi.org/10.1038/s41419-017-0212-3 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Yang, Cheng-Ta Li, Jhy-Ming Chu, Wing-Keung Chow, Shu-Er Downregulation of lumican accelerates lung cancer cell invasion through p120 catenin |
title | Downregulation of lumican accelerates lung cancer cell invasion through p120 catenin |
title_full | Downregulation of lumican accelerates lung cancer cell invasion through p120 catenin |
title_fullStr | Downregulation of lumican accelerates lung cancer cell invasion through p120 catenin |
title_full_unstemmed | Downregulation of lumican accelerates lung cancer cell invasion through p120 catenin |
title_short | Downregulation of lumican accelerates lung cancer cell invasion through p120 catenin |
title_sort | downregulation of lumican accelerates lung cancer cell invasion through p120 catenin |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5856799/ https://www.ncbi.nlm.nih.gov/pubmed/29549325 http://dx.doi.org/10.1038/s41419-017-0212-3 |
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