Cargando…

Downregulation of lumican accelerates lung cancer cell invasion through p120 catenin

The overexpression of lumican has been found in lung cancer cells; however, the functional role of lumican in lung cancer cells remains unclear. In this study, we found lumican functioned as a tubulin-binding protein and the depletion of lumican by transfection with its specific shRNA increased lung...

Descripción completa

Detalles Bibliográficos
Autores principales: Yang, Cheng-Ta, Li, Jhy-Ming, Chu, Wing-Keung, Chow, Shu-Er
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5856799/
https://www.ncbi.nlm.nih.gov/pubmed/29549325
http://dx.doi.org/10.1038/s41419-017-0212-3
_version_ 1783307342566129664
author Yang, Cheng-Ta
Li, Jhy-Ming
Chu, Wing-Keung
Chow, Shu-Er
author_facet Yang, Cheng-Ta
Li, Jhy-Ming
Chu, Wing-Keung
Chow, Shu-Er
author_sort Yang, Cheng-Ta
collection PubMed
description The overexpression of lumican has been found in lung cancer cells; however, the functional role of lumican in lung cancer cells remains unclear. In this study, we found lumican functioned as a tubulin-binding protein and the depletion of lumican by transfection with its specific shRNA increased lung cancer cell invasion. Such alterations led to morphological changes and actin cytoskeleton remodeling, including the induction of membrane ruffling or protrusion and stress fiber formation, correlated with the increased activities of Rac and Rho. The downregulation of lumican was also implicated in macrophage-conditioned media (maCM)-induced cell invasion. Immunofluorescence images and immunoprecipitation assays revealed the co-localization of p120-catenin (p120ctn) and lumican. Reduction in the levels of p120ctn induced membrane ruffling and the activation of the Rho family, which accelerated cell invasion. Our data indicated that lumican is associated with microtubule-modulated p120ctn signaling, providing important insights into lung cancer progression.
format Online
Article
Text
id pubmed-5856799
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher Nature Publishing Group UK
record_format MEDLINE/PubMed
spelling pubmed-58567992018-05-31 Downregulation of lumican accelerates lung cancer cell invasion through p120 catenin Yang, Cheng-Ta Li, Jhy-Ming Chu, Wing-Keung Chow, Shu-Er Cell Death Dis Article The overexpression of lumican has been found in lung cancer cells; however, the functional role of lumican in lung cancer cells remains unclear. In this study, we found lumican functioned as a tubulin-binding protein and the depletion of lumican by transfection with its specific shRNA increased lung cancer cell invasion. Such alterations led to morphological changes and actin cytoskeleton remodeling, including the induction of membrane ruffling or protrusion and stress fiber formation, correlated with the increased activities of Rac and Rho. The downregulation of lumican was also implicated in macrophage-conditioned media (maCM)-induced cell invasion. Immunofluorescence images and immunoprecipitation assays revealed the co-localization of p120-catenin (p120ctn) and lumican. Reduction in the levels of p120ctn induced membrane ruffling and the activation of the Rho family, which accelerated cell invasion. Our data indicated that lumican is associated with microtubule-modulated p120ctn signaling, providing important insights into lung cancer progression. Nature Publishing Group UK 2018-03-16 /pmc/articles/PMC5856799/ /pubmed/29549325 http://dx.doi.org/10.1038/s41419-017-0212-3 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Yang, Cheng-Ta
Li, Jhy-Ming
Chu, Wing-Keung
Chow, Shu-Er
Downregulation of lumican accelerates lung cancer cell invasion through p120 catenin
title Downregulation of lumican accelerates lung cancer cell invasion through p120 catenin
title_full Downregulation of lumican accelerates lung cancer cell invasion through p120 catenin
title_fullStr Downregulation of lumican accelerates lung cancer cell invasion through p120 catenin
title_full_unstemmed Downregulation of lumican accelerates lung cancer cell invasion through p120 catenin
title_short Downregulation of lumican accelerates lung cancer cell invasion through p120 catenin
title_sort downregulation of lumican accelerates lung cancer cell invasion through p120 catenin
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5856799/
https://www.ncbi.nlm.nih.gov/pubmed/29549325
http://dx.doi.org/10.1038/s41419-017-0212-3
work_keys_str_mv AT yangchengta downregulationoflumicanaccelerateslungcancercellinvasionthroughp120catenin
AT lijhyming downregulationoflumicanaccelerateslungcancercellinvasionthroughp120catenin
AT chuwingkeung downregulationoflumicanaccelerateslungcancercellinvasionthroughp120catenin
AT chowshuer downregulationoflumicanaccelerateslungcancercellinvasionthroughp120catenin