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Careful use of 16S rRNA gene sequence similarity values for the identification of Mycobacterium species
In order to evaluate the suitability of 16S rRNA nucleotide sequence similarity for the classification of new Mycobacterium isolates at the species level, we systematically studied the pairwise identity values of this gene for 131 Mycobacterium species with standing in nomenclature. Only one of the...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5857167/ https://www.ncbi.nlm.nih.gov/pubmed/29556405 http://dx.doi.org/10.1016/j.nmni.2017.12.009 |
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author | Beye, M. Fahsi, N. Raoult, D. Fournier, P.-E. |
author_facet | Beye, M. Fahsi, N. Raoult, D. Fournier, P.-E. |
author_sort | Beye, M. |
collection | PubMed |
description | In order to evaluate the suitability of 16S rRNA nucleotide sequence similarity for the classification of new Mycobacterium isolates at the species level, we systematically studied the pairwise identity values of this gene for 131 Mycobacterium species with standing in nomenclature. Only one of the studied species, M. poriferae (0.76%), strictly respected the 95% and 98.65% threshold values currently recommended to determine the affiliation of bacterial isolates to an existing or new genus or species, respectively. All other species exhibited at least an identity value >98.65% and/or <95% with another Mycobacterium species. Therefore, we suggest that interpretation of interspecies 16S rRNA identity values should be made cautiously when classifying a new mycobacterial isolate at the species level. |
format | Online Article Text |
id | pubmed-5857167 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-58571672018-03-19 Careful use of 16S rRNA gene sequence similarity values for the identification of Mycobacterium species Beye, M. Fahsi, N. Raoult, D. Fournier, P.-E. New Microbes New Infect Original Article In order to evaluate the suitability of 16S rRNA nucleotide sequence similarity for the classification of new Mycobacterium isolates at the species level, we systematically studied the pairwise identity values of this gene for 131 Mycobacterium species with standing in nomenclature. Only one of the studied species, M. poriferae (0.76%), strictly respected the 95% and 98.65% threshold values currently recommended to determine the affiliation of bacterial isolates to an existing or new genus or species, respectively. All other species exhibited at least an identity value >98.65% and/or <95% with another Mycobacterium species. Therefore, we suggest that interpretation of interspecies 16S rRNA identity values should be made cautiously when classifying a new mycobacterial isolate at the species level. Elsevier 2017-12-29 /pmc/articles/PMC5857167/ /pubmed/29556405 http://dx.doi.org/10.1016/j.nmni.2017.12.009 Text en © 2017 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Original Article Beye, M. Fahsi, N. Raoult, D. Fournier, P.-E. Careful use of 16S rRNA gene sequence similarity values for the identification of Mycobacterium species |
title | Careful use of 16S rRNA gene sequence similarity values for the identification of Mycobacterium species |
title_full | Careful use of 16S rRNA gene sequence similarity values for the identification of Mycobacterium species |
title_fullStr | Careful use of 16S rRNA gene sequence similarity values for the identification of Mycobacterium species |
title_full_unstemmed | Careful use of 16S rRNA gene sequence similarity values for the identification of Mycobacterium species |
title_short | Careful use of 16S rRNA gene sequence similarity values for the identification of Mycobacterium species |
title_sort | careful use of 16s rrna gene sequence similarity values for the identification of mycobacterium species |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5857167/ https://www.ncbi.nlm.nih.gov/pubmed/29556405 http://dx.doi.org/10.1016/j.nmni.2017.12.009 |
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