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Low-Level Laser Therapy Reduces Lung Inflammation in an Experimental Model of Chronic Obstructive Pulmonary Disease Involving P2X7 Receptor
Chronic obstructive pulmonary disease (COPD) is a progressive disease characterized by irreversible airflow limitation, airway inflammation and remodeling, and enlargement of alveolar spaces. COPD is in the top five leading causes of deaths worldwide and presents a high economic cost. However, there...
Autores principales: | , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5857317/ https://www.ncbi.nlm.nih.gov/pubmed/29686745 http://dx.doi.org/10.1155/2018/6798238 |
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author | da Cunha Moraes, Gabriel Vitoretti, Luana Beatriz de Brito, Auriléia Aparecida Alves, Cintia Estefano de Oliveira, Nicole Cristine Rigonato dos Santos Dias, Alana Matos, Yves Silva Teles Oliveira-Junior, Manoel Carneiro Oliveira, Luis Vicente Franco da Palma, Renata Kelly Candeo, Larissa Carbonera Lino-dos-Santos-Franco, Adriana Horliana, Anna Carolina Ratto Tempestine Gimenes Júnior, João Antonio Aimbire, Flavio Vieira, Rodolfo Paula Ligeiro-de-Oliveira, Ana Paula |
author_facet | da Cunha Moraes, Gabriel Vitoretti, Luana Beatriz de Brito, Auriléia Aparecida Alves, Cintia Estefano de Oliveira, Nicole Cristine Rigonato dos Santos Dias, Alana Matos, Yves Silva Teles Oliveira-Junior, Manoel Carneiro Oliveira, Luis Vicente Franco da Palma, Renata Kelly Candeo, Larissa Carbonera Lino-dos-Santos-Franco, Adriana Horliana, Anna Carolina Ratto Tempestine Gimenes Júnior, João Antonio Aimbire, Flavio Vieira, Rodolfo Paula Ligeiro-de-Oliveira, Ana Paula |
author_sort | da Cunha Moraes, Gabriel |
collection | PubMed |
description | Chronic obstructive pulmonary disease (COPD) is a progressive disease characterized by irreversible airflow limitation, airway inflammation and remodeling, and enlargement of alveolar spaces. COPD is in the top five leading causes of deaths worldwide and presents a high economic cost. However, there are some preventive measures to lower the risk of developing COPD. Low-level laser therapy (LLLT) is a new effective therapy, with very low cost and no side effects. So, our objective was to investigate if LLLT reduces pulmonary alterations in an experimental model of COPD. C57BL/6 mice were submitted to cigarette smoke for 75 days (2x/day). After 60 days to smoke exposure, the treated group was submitted to LLLT (diode laser, 660 nm, 30 mW, and 3 J/cm(2)) for 15 days and euthanized for morphologic and functional analysis of the lungs. Our results showed that LLLT significantly reduced the number of inflammatory cells and the proinflammatory cytokine secretion such as IL-1β, IL-6, and TNF-α in bronchoalveolar lavage fluid (BALF). We also observed that LLLT decreased collagen deposition as well as the expression of purinergic P2X7 receptor. On the other hand, LLLT increased the IL-10 release. Thus, LLLT can be pointed as a promising therapeutic approach for lung inflammatory diseases as COPD. |
format | Online Article Text |
id | pubmed-5857317 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Hindawi |
record_format | MEDLINE/PubMed |
spelling | pubmed-58573172018-04-23 Low-Level Laser Therapy Reduces Lung Inflammation in an Experimental Model of Chronic Obstructive Pulmonary Disease Involving P2X7 Receptor da Cunha Moraes, Gabriel Vitoretti, Luana Beatriz de Brito, Auriléia Aparecida Alves, Cintia Estefano de Oliveira, Nicole Cristine Rigonato dos Santos Dias, Alana Matos, Yves Silva Teles Oliveira-Junior, Manoel Carneiro Oliveira, Luis Vicente Franco da Palma, Renata Kelly Candeo, Larissa Carbonera Lino-dos-Santos-Franco, Adriana Horliana, Anna Carolina Ratto Tempestine Gimenes Júnior, João Antonio Aimbire, Flavio Vieira, Rodolfo Paula Ligeiro-de-Oliveira, Ana Paula Oxid Med Cell Longev Research Article Chronic obstructive pulmonary disease (COPD) is a progressive disease characterized by irreversible airflow limitation, airway inflammation and remodeling, and enlargement of alveolar spaces. COPD is in the top five leading causes of deaths worldwide and presents a high economic cost. However, there are some preventive measures to lower the risk of developing COPD. Low-level laser therapy (LLLT) is a new effective therapy, with very low cost and no side effects. So, our objective was to investigate if LLLT reduces pulmonary alterations in an experimental model of COPD. C57BL/6 mice were submitted to cigarette smoke for 75 days (2x/day). After 60 days to smoke exposure, the treated group was submitted to LLLT (diode laser, 660 nm, 30 mW, and 3 J/cm(2)) for 15 days and euthanized for morphologic and functional analysis of the lungs. Our results showed that LLLT significantly reduced the number of inflammatory cells and the proinflammatory cytokine secretion such as IL-1β, IL-6, and TNF-α in bronchoalveolar lavage fluid (BALF). We also observed that LLLT decreased collagen deposition as well as the expression of purinergic P2X7 receptor. On the other hand, LLLT increased the IL-10 release. Thus, LLLT can be pointed as a promising therapeutic approach for lung inflammatory diseases as COPD. Hindawi 2018-03-04 /pmc/articles/PMC5857317/ /pubmed/29686745 http://dx.doi.org/10.1155/2018/6798238 Text en Copyright © 2018 Gabriel da Cunha Moraes et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article da Cunha Moraes, Gabriel Vitoretti, Luana Beatriz de Brito, Auriléia Aparecida Alves, Cintia Estefano de Oliveira, Nicole Cristine Rigonato dos Santos Dias, Alana Matos, Yves Silva Teles Oliveira-Junior, Manoel Carneiro Oliveira, Luis Vicente Franco da Palma, Renata Kelly Candeo, Larissa Carbonera Lino-dos-Santos-Franco, Adriana Horliana, Anna Carolina Ratto Tempestine Gimenes Júnior, João Antonio Aimbire, Flavio Vieira, Rodolfo Paula Ligeiro-de-Oliveira, Ana Paula Low-Level Laser Therapy Reduces Lung Inflammation in an Experimental Model of Chronic Obstructive Pulmonary Disease Involving P2X7 Receptor |
title | Low-Level Laser Therapy Reduces Lung Inflammation in an Experimental Model of Chronic Obstructive Pulmonary Disease Involving P2X7 Receptor |
title_full | Low-Level Laser Therapy Reduces Lung Inflammation in an Experimental Model of Chronic Obstructive Pulmonary Disease Involving P2X7 Receptor |
title_fullStr | Low-Level Laser Therapy Reduces Lung Inflammation in an Experimental Model of Chronic Obstructive Pulmonary Disease Involving P2X7 Receptor |
title_full_unstemmed | Low-Level Laser Therapy Reduces Lung Inflammation in an Experimental Model of Chronic Obstructive Pulmonary Disease Involving P2X7 Receptor |
title_short | Low-Level Laser Therapy Reduces Lung Inflammation in an Experimental Model of Chronic Obstructive Pulmonary Disease Involving P2X7 Receptor |
title_sort | low-level laser therapy reduces lung inflammation in an experimental model of chronic obstructive pulmonary disease involving p2x7 receptor |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5857317/ https://www.ncbi.nlm.nih.gov/pubmed/29686745 http://dx.doi.org/10.1155/2018/6798238 |
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