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Antibody secreting B cells and plasma antibody response to rotavirus vaccination in infants from Kolkata India

BACKGROUND: Assessing immune response after rotavirus vaccination consists in measuring serum or plasma IgA and IgG antibodies, but these assays provide very little information about the mucosal immune response. Thus the development of assays for detection of mucosal immune response following rotavi...

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Autores principales: Sinha, Anuradha, Kanungo, Suman, Kim, Deok Ryun, Manna, Byomkesh, Song, Manki, Park, Ju Yeon, Haldar, Bisakha, Sharma, Prashant, Mallick, Aiyel Haque, Kim, Soon Ae, Babji, Sudhir, Sur, Dipika, Kang, Gagandeep, Ali, Mohammad, Petri Jr., William A, Wierzba, Thomas F, Czerkinsky, Cecil, Nandy, Ranjan Kumar, Dey, Ayan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5857522/
https://www.ncbi.nlm.nih.gov/pubmed/29560435
http://dx.doi.org/10.1016/j.heliyon.2018.e00519
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author Sinha, Anuradha
Kanungo, Suman
Kim, Deok Ryun
Manna, Byomkesh
Song, Manki
Park, Ju Yeon
Haldar, Bisakha
Sharma, Prashant
Mallick, Aiyel Haque
Kim, Soon Ae
Babji, Sudhir
Sur, Dipika
Kang, Gagandeep
Ali, Mohammad
Petri Jr., William A
Wierzba, Thomas F
Czerkinsky, Cecil
Nandy, Ranjan Kumar
Dey, Ayan
author_facet Sinha, Anuradha
Kanungo, Suman
Kim, Deok Ryun
Manna, Byomkesh
Song, Manki
Park, Ju Yeon
Haldar, Bisakha
Sharma, Prashant
Mallick, Aiyel Haque
Kim, Soon Ae
Babji, Sudhir
Sur, Dipika
Kang, Gagandeep
Ali, Mohammad
Petri Jr., William A
Wierzba, Thomas F
Czerkinsky, Cecil
Nandy, Ranjan Kumar
Dey, Ayan
author_sort Sinha, Anuradha
collection PubMed
description BACKGROUND: Assessing immune response after rotavirus vaccination consists in measuring serum or plasma IgA and IgG antibodies, but these assays provide very little information about the mucosal immune response. Thus the development of assays for detection of mucosal immune response following rotavirus vaccination is essential. We evaluate to assess circulating antibody-secreting cells (ASCs) as a potential means to evaluate mucosal immune responses to rotavirus vaccine. METHODS: 372 subjects, aged 6 weeks, were enrolled in the study. All the subjects were assigned to receive two doses of Rotarix(®) vaccine. Using a micro-modified whole blood-based ELISPOT assay, circulating rotavirus type-specific IgA- and IgG-ASCs, including gut homing β7+ ASCs, were enumerated on week 6 before the first dose of Rotarix vaccination at 7 weeks of age and week 18 after the second vaccination at 17 weeks of age. Plasma samples collected before vaccination, and after two doses of Rotarix(®) vaccination were tested for plasma rotavirus IgA titers. RESULTS: Two doses of Rotarix(®) provided to induce sero-protective titer of ≥ 20 Units in 35% of subjects. Total blood IgA- ASC responses were detected in 26.4% of subjects who were non-responder before vaccination. Among responders, 47% of the subjects also have sero-protective plasma IgA titers. DISCUSSION: Our results suggest that virus-specific blood gut homing ASCs were detected and provide insight into mucosal immune response after rotavirus vaccination. Further studies are needed to evaluate the duration of such immune responses and to assess the programmatic utility of this whole blood-based mucosal ASC testing for the rotavirus immunization program.
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spelling pubmed-58575222018-03-20 Antibody secreting B cells and plasma antibody response to rotavirus vaccination in infants from Kolkata India Sinha, Anuradha Kanungo, Suman Kim, Deok Ryun Manna, Byomkesh Song, Manki Park, Ju Yeon Haldar, Bisakha Sharma, Prashant Mallick, Aiyel Haque Kim, Soon Ae Babji, Sudhir Sur, Dipika Kang, Gagandeep Ali, Mohammad Petri Jr., William A Wierzba, Thomas F Czerkinsky, Cecil Nandy, Ranjan Kumar Dey, Ayan Heliyon Article BACKGROUND: Assessing immune response after rotavirus vaccination consists in measuring serum or plasma IgA and IgG antibodies, but these assays provide very little information about the mucosal immune response. Thus the development of assays for detection of mucosal immune response following rotavirus vaccination is essential. We evaluate to assess circulating antibody-secreting cells (ASCs) as a potential means to evaluate mucosal immune responses to rotavirus vaccine. METHODS: 372 subjects, aged 6 weeks, were enrolled in the study. All the subjects were assigned to receive two doses of Rotarix(®) vaccine. Using a micro-modified whole blood-based ELISPOT assay, circulating rotavirus type-specific IgA- and IgG-ASCs, including gut homing β7+ ASCs, were enumerated on week 6 before the first dose of Rotarix vaccination at 7 weeks of age and week 18 after the second vaccination at 17 weeks of age. Plasma samples collected before vaccination, and after two doses of Rotarix(®) vaccination were tested for plasma rotavirus IgA titers. RESULTS: Two doses of Rotarix(®) provided to induce sero-protective titer of ≥ 20 Units in 35% of subjects. Total blood IgA- ASC responses were detected in 26.4% of subjects who were non-responder before vaccination. Among responders, 47% of the subjects also have sero-protective plasma IgA titers. DISCUSSION: Our results suggest that virus-specific blood gut homing ASCs were detected and provide insight into mucosal immune response after rotavirus vaccination. Further studies are needed to evaluate the duration of such immune responses and to assess the programmatic utility of this whole blood-based mucosal ASC testing for the rotavirus immunization program. Elsevier 2018-02-01 /pmc/articles/PMC5857522/ /pubmed/29560435 http://dx.doi.org/10.1016/j.heliyon.2018.e00519 Text en © 2018 The Authors http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Sinha, Anuradha
Kanungo, Suman
Kim, Deok Ryun
Manna, Byomkesh
Song, Manki
Park, Ju Yeon
Haldar, Bisakha
Sharma, Prashant
Mallick, Aiyel Haque
Kim, Soon Ae
Babji, Sudhir
Sur, Dipika
Kang, Gagandeep
Ali, Mohammad
Petri Jr., William A
Wierzba, Thomas F
Czerkinsky, Cecil
Nandy, Ranjan Kumar
Dey, Ayan
Antibody secreting B cells and plasma antibody response to rotavirus vaccination in infants from Kolkata India
title Antibody secreting B cells and plasma antibody response to rotavirus vaccination in infants from Kolkata India
title_full Antibody secreting B cells and plasma antibody response to rotavirus vaccination in infants from Kolkata India
title_fullStr Antibody secreting B cells and plasma antibody response to rotavirus vaccination in infants from Kolkata India
title_full_unstemmed Antibody secreting B cells and plasma antibody response to rotavirus vaccination in infants from Kolkata India
title_short Antibody secreting B cells and plasma antibody response to rotavirus vaccination in infants from Kolkata India
title_sort antibody secreting b cells and plasma antibody response to rotavirus vaccination in infants from kolkata india
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5857522/
https://www.ncbi.nlm.nih.gov/pubmed/29560435
http://dx.doi.org/10.1016/j.heliyon.2018.e00519
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