Closed tube loop-mediated isothermal amplification assay for rapid detection of hepatitis B virus in human blood

Recently, many studies have demonstrated the significant advantages of loop-mediated isothermal amplification (LAMP) based methods over serological tests and PCR for rapid detection of microbial pathogens. Here, a rapid LAMP assay was developed to detect the hepatitis B virus (HBV) from DNA, and par...

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Detalles Bibliográficos
Autores principales: Quoc, Nguyen Bao, Phuong, Nguyen Doan Nguyen, Chau, Nguyen Ngoc Bao, Linh, Do Thi Phuong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2018
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5857714/
https://www.ncbi.nlm.nih.gov/pubmed/29560471
http://dx.doi.org/10.1016/j.heliyon.2018.e00561
Descripción
Sumario:Recently, many studies have demonstrated the significant advantages of loop-mediated isothermal amplification (LAMP) based methods over serological tests and PCR for rapid detection of microbial pathogens. Here, a rapid LAMP assay was developed to detect the hepatitis B virus (HBV) from DNA, and particularly, blood samples from infected patients using a commercially available master mix and portable real-time fluorometer. The final optimized fluorescence-based LAMP assay provided significant amplification time of less than 15 minutes compared with over 1 hour for PCR and an opened tube LAMP system described previously. Results indicated that fluorescence-based LAMP assay was more sensitive than PCR as a rapid, sensitive, efficient, and highly reliable approach for rapid detection of HBV.

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