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LncRNA GAS5 suppresses ovarian cancer by inducing inflammasome formation

Objective: Long noncoding RNA growth arrest-specific transcript 5 (lncRNA GAS5) is involved in various kinds of cancer. However, the role of lncGAS5 in the development of ovarian cancer remains unclear. In the present study, we explored the cellular mechanism and clinical value of lncRNA GAS5 in ova...

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Autores principales: Li, Jie, Yang, Chen, Li, Yinguang, Chen, Aiyue, Li, Li, You, Zeshan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Portland Press Ltd. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5857912/
https://www.ncbi.nlm.nih.gov/pubmed/29229673
http://dx.doi.org/10.1042/BSR20171150
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author Li, Jie
Yang, Chen
Li, Yinguang
Chen, Aiyue
Li, Li
You, Zeshan
author_facet Li, Jie
Yang, Chen
Li, Yinguang
Chen, Aiyue
Li, Li
You, Zeshan
author_sort Li, Jie
collection PubMed
description Objective: Long noncoding RNA growth arrest-specific transcript 5 (lncRNA GAS5) is involved in various kinds of cancer. However, the role of lncGAS5 in the development of ovarian cancer remains unclear. In the present study, we explored the cellular mechanism and clinical value of lncRNA GAS5 in ovarian cancer. Methods: Quantitative real-time PCR was used to detect mRNA level of lncRNA GAS5 in 20 ovarian cancer tissues. The effect of lncRNA GAS5 on cell proliferation was performed using CCK-8 assay. Cell apoptosis was evaluated by flow cytometry. Western blotting was used to detect the protein level of lncRNA GAS5 potential target. Standard sandwich ELISA was used to quantify the level of inflammatory cytokines. The cells with stable expression of lncRNA GAS5 were injected into nude mice to study the effect of lncRNA GAS5 on tumorigenesis in vivo. Results: The expression of lncRNA GAS5 was significantly decreased in ovarian cancer tissues. Decrease in lncRNA GAS5 expression resulted in increased cell proliferation and colony formation and reduced ovarian cancer cell apoptosis. In contrast, exogenous overexpression of lncRNA GAS5 in ovarian cancer cells inhibited proliferation, colony formation, and apoptosis in ovarian cancer cells. In addition, the role of lncRNA GAS5 in ovarian cancer was associated with inflammasome formation and pyroptosis. Conclusion: These results suggested that lncRNA GAS5 acts as tumor suppressor and could be used as a potential treatment target for diagnosis and therapy of ovarian cancer.
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spelling pubmed-58579122018-03-29 LncRNA GAS5 suppresses ovarian cancer by inducing inflammasome formation Li, Jie Yang, Chen Li, Yinguang Chen, Aiyue Li, Li You, Zeshan Biosci Rep Research Articles Objective: Long noncoding RNA growth arrest-specific transcript 5 (lncRNA GAS5) is involved in various kinds of cancer. However, the role of lncGAS5 in the development of ovarian cancer remains unclear. In the present study, we explored the cellular mechanism and clinical value of lncRNA GAS5 in ovarian cancer. Methods: Quantitative real-time PCR was used to detect mRNA level of lncRNA GAS5 in 20 ovarian cancer tissues. The effect of lncRNA GAS5 on cell proliferation was performed using CCK-8 assay. Cell apoptosis was evaluated by flow cytometry. Western blotting was used to detect the protein level of lncRNA GAS5 potential target. Standard sandwich ELISA was used to quantify the level of inflammatory cytokines. The cells with stable expression of lncRNA GAS5 were injected into nude mice to study the effect of lncRNA GAS5 on tumorigenesis in vivo. Results: The expression of lncRNA GAS5 was significantly decreased in ovarian cancer tissues. Decrease in lncRNA GAS5 expression resulted in increased cell proliferation and colony formation and reduced ovarian cancer cell apoptosis. In contrast, exogenous overexpression of lncRNA GAS5 in ovarian cancer cells inhibited proliferation, colony formation, and apoptosis in ovarian cancer cells. In addition, the role of lncRNA GAS5 in ovarian cancer was associated with inflammasome formation and pyroptosis. Conclusion: These results suggested that lncRNA GAS5 acts as tumor suppressor and could be used as a potential treatment target for diagnosis and therapy of ovarian cancer. Portland Press Ltd. 2018-03-16 /pmc/articles/PMC5857912/ /pubmed/29229673 http://dx.doi.org/10.1042/BSR20171150 Text en © 2018 The Author(s). http://creativecommons.org/licenses/by/4.0/This is an open access article published by Portland Press Limited on behalf of the Biochemical Society and distributed under the Creative Commons Attribution License 4.0 (CC BY) (http://creativecommons.org/licenses/by/4.0/) .
spellingShingle Research Articles
Li, Jie
Yang, Chen
Li, Yinguang
Chen, Aiyue
Li, Li
You, Zeshan
LncRNA GAS5 suppresses ovarian cancer by inducing inflammasome formation
title LncRNA GAS5 suppresses ovarian cancer by inducing inflammasome formation
title_full LncRNA GAS5 suppresses ovarian cancer by inducing inflammasome formation
title_fullStr LncRNA GAS5 suppresses ovarian cancer by inducing inflammasome formation
title_full_unstemmed LncRNA GAS5 suppresses ovarian cancer by inducing inflammasome formation
title_short LncRNA GAS5 suppresses ovarian cancer by inducing inflammasome formation
title_sort lncrna gas5 suppresses ovarian cancer by inducing inflammasome formation
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5857912/
https://www.ncbi.nlm.nih.gov/pubmed/29229673
http://dx.doi.org/10.1042/BSR20171150
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