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Protective effects of paeonol on subacute/chronic brain injury during cerebral ischemia in rats

Ischemic stroke is a highly complex pathological process that is divided into acute, subacute and chronic phases. Paeonol is a biologically active natural product with a variety of pharmacological effects, including those on neuronal activity. However, the effects of paeonol on subacute/chronic isch...

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Autores principales: Zhao, Bing, Shi, Qiao-Juan, Zhang, Zhen-Zhong, Wang, Shu-Yan, Wang, Xi, Wang, Hao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5858057/
https://www.ncbi.nlm.nih.gov/pubmed/29563983
http://dx.doi.org/10.3892/etm.2018.5893
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author Zhao, Bing
Shi, Qiao-Juan
Zhang, Zhen-Zhong
Wang, Shu-Yan
Wang, Xi
Wang, Hao
author_facet Zhao, Bing
Shi, Qiao-Juan
Zhang, Zhen-Zhong
Wang, Shu-Yan
Wang, Xi
Wang, Hao
author_sort Zhao, Bing
collection PubMed
description Ischemic stroke is a highly complex pathological process that is divided into acute, subacute and chronic phases. Paeonol is a biologically active natural product with a variety of pharmacological effects, including those on neuronal activity. However, the effects of paeonol on subacute/chronic ischemic stroke have remained to be elucidated. The present study was designed to investigate the effects of paeonol against subacute and chronic cerebral ischemic injury and to explore the possible underlying mechanisms. Male adult Sprague Dawley rats were randomly divided into a sham group (treated with saline), a model group [subjected to middle cerebral artery occlusion (MCAO) and treated with saline] and a paeonol-treated group (MCAO + paeonol at 25 mg/kg). Behavioral impairment, infarct volume and ischemic/contralateral hemispheric ratios were assessed at 72 h and at 28 days after MCAO, respectively. Immunofluorescence was employed to determine the neuronal damage and glial responses after MCAO. Compared with the model group, paeonol treatment significantly attenuated behavioral impairment, ischemic infarct volume and moderate cerebral edema in the ischemic brain at 72 h, as well as brain atrophy at 28 days after reperfusion. Furthermore, paeonol treatment ameliorated neuronal damage in the ischemic core and boundary zone regions at 72 h after reperfusion and in the boundary zone at 28 days after reperfusion. In addition, paeonol treatment reduced the proliferation of astrocytes in the boundary zone, and inhibited microglial activation in the ischemic core and boundary zone regions at 72 h and 28 days after reperfusion. These results demonstrated the protective effects of paeonol against subacute/chronic cerebral ischemia, and the mechanism of action may include subacute/chronic microglial activation and astrocyte proliferation.
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spelling pubmed-58580572018-03-21 Protective effects of paeonol on subacute/chronic brain injury during cerebral ischemia in rats Zhao, Bing Shi, Qiao-Juan Zhang, Zhen-Zhong Wang, Shu-Yan Wang, Xi Wang, Hao Exp Ther Med Articles Ischemic stroke is a highly complex pathological process that is divided into acute, subacute and chronic phases. Paeonol is a biologically active natural product with a variety of pharmacological effects, including those on neuronal activity. However, the effects of paeonol on subacute/chronic ischemic stroke have remained to be elucidated. The present study was designed to investigate the effects of paeonol against subacute and chronic cerebral ischemic injury and to explore the possible underlying mechanisms. Male adult Sprague Dawley rats were randomly divided into a sham group (treated with saline), a model group [subjected to middle cerebral artery occlusion (MCAO) and treated with saline] and a paeonol-treated group (MCAO + paeonol at 25 mg/kg). Behavioral impairment, infarct volume and ischemic/contralateral hemispheric ratios were assessed at 72 h and at 28 days after MCAO, respectively. Immunofluorescence was employed to determine the neuronal damage and glial responses after MCAO. Compared with the model group, paeonol treatment significantly attenuated behavioral impairment, ischemic infarct volume and moderate cerebral edema in the ischemic brain at 72 h, as well as brain atrophy at 28 days after reperfusion. Furthermore, paeonol treatment ameliorated neuronal damage in the ischemic core and boundary zone regions at 72 h after reperfusion and in the boundary zone at 28 days after reperfusion. In addition, paeonol treatment reduced the proliferation of astrocytes in the boundary zone, and inhibited microglial activation in the ischemic core and boundary zone regions at 72 h and 28 days after reperfusion. These results demonstrated the protective effects of paeonol against subacute/chronic cerebral ischemia, and the mechanism of action may include subacute/chronic microglial activation and astrocyte proliferation. D.A. Spandidos 2018-04 2018-02-26 /pmc/articles/PMC5858057/ /pubmed/29563983 http://dx.doi.org/10.3892/etm.2018.5893 Text en Copyright: © Zhao et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Zhao, Bing
Shi, Qiao-Juan
Zhang, Zhen-Zhong
Wang, Shu-Yan
Wang, Xi
Wang, Hao
Protective effects of paeonol on subacute/chronic brain injury during cerebral ischemia in rats
title Protective effects of paeonol on subacute/chronic brain injury during cerebral ischemia in rats
title_full Protective effects of paeonol on subacute/chronic brain injury during cerebral ischemia in rats
title_fullStr Protective effects of paeonol on subacute/chronic brain injury during cerebral ischemia in rats
title_full_unstemmed Protective effects of paeonol on subacute/chronic brain injury during cerebral ischemia in rats
title_short Protective effects of paeonol on subacute/chronic brain injury during cerebral ischemia in rats
title_sort protective effects of paeonol on subacute/chronic brain injury during cerebral ischemia in rats
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5858057/
https://www.ncbi.nlm.nih.gov/pubmed/29563983
http://dx.doi.org/10.3892/etm.2018.5893
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