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The expression of the new epididymal luminal protein of PDZ domain containing 1 is decreased in asthenozoospermia

Spermatozoa are not mature until they transit the epididymis where they acquire motility and the ability to fertilize an egg through sequential modifications. The epididymis has three functional regions, caput, corpus, and cauda, and the luminal proteins of the epididymis play important roles in the...

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Autores principales: Liang, A-Juan, Wang, Gui-Shuan, Ping, Ping, Hu, Shuang-Gang, Lin, Yu, Ma, Yi, Duan, Zheng-Zheng, Wang, Han-Shu, Sun, Fei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medknow Publications & Media Pvt Ltd 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5858100/
https://www.ncbi.nlm.nih.gov/pubmed/29405165
http://dx.doi.org/10.4103/aja.aja_65_17
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author Liang, A-Juan
Wang, Gui-Shuan
Ping, Ping
Hu, Shuang-Gang
Lin, Yu
Ma, Yi
Duan, Zheng-Zheng
Wang, Han-Shu
Sun, Fei
author_facet Liang, A-Juan
Wang, Gui-Shuan
Ping, Ping
Hu, Shuang-Gang
Lin, Yu
Ma, Yi
Duan, Zheng-Zheng
Wang, Han-Shu
Sun, Fei
author_sort Liang, A-Juan
collection PubMed
description Spermatozoa are not mature until they transit the epididymis where they acquire motility and the ability to fertilize an egg through sequential modifications. The epididymis has three functional regions, caput, corpus, and cauda, and the luminal proteins of the epididymis play important roles in the above modifications. However, the proteins with differential enrichment between the caput and cauda are still largely unknown. To reveal the functions of the caput and cauda during sperm maturation, luminal proteins from caput and cauda of mice were analyzed by isobaric tag for relative and absolute quantitation (iTRAQ). Overall, 128 differentially enriched proteins were found, of which 46 were caput enriched and 82 were cauda enriched. Bioinformatic analysis showed that lipid metabolism was active in the caput; while anion- and cation-binding activity and phosphorus and organophosphate metabolism were active in the cauda. A new epididymal luminal protein, the caput-enriched PDZ domain containing 1 (Pdzk1), also named Na(+)/H(+) exchange regulatory cofactor 3 (NHERF3), which plays a critical role in cholesterol metabolism and carnitine transport, was found in the lipid metabolism. Western blotting and immunofluorescence analyses showed that Pdzk1 was expressed in the epididymis but not in the testis, and localized at the middle piece of the sperm tail. Pdzk1 protein level was also reduced in the spermatozoa in case of asthenozoospermic patients compared with that in normozoospermic men, suggesting that Pdzk1 may participate in sperm maturation regulation and may be associated with male infertility. These results may provide new insights into the mechanisms of sperm maturation and male infertility.
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spelling pubmed-58581002018-03-23 The expression of the new epididymal luminal protein of PDZ domain containing 1 is decreased in asthenozoospermia Liang, A-Juan Wang, Gui-Shuan Ping, Ping Hu, Shuang-Gang Lin, Yu Ma, Yi Duan, Zheng-Zheng Wang, Han-Shu Sun, Fei Asian J Androl Original Article Spermatozoa are not mature until they transit the epididymis where they acquire motility and the ability to fertilize an egg through sequential modifications. The epididymis has three functional regions, caput, corpus, and cauda, and the luminal proteins of the epididymis play important roles in the above modifications. However, the proteins with differential enrichment between the caput and cauda are still largely unknown. To reveal the functions of the caput and cauda during sperm maturation, luminal proteins from caput and cauda of mice were analyzed by isobaric tag for relative and absolute quantitation (iTRAQ). Overall, 128 differentially enriched proteins were found, of which 46 were caput enriched and 82 were cauda enriched. Bioinformatic analysis showed that lipid metabolism was active in the caput; while anion- and cation-binding activity and phosphorus and organophosphate metabolism were active in the cauda. A new epididymal luminal protein, the caput-enriched PDZ domain containing 1 (Pdzk1), also named Na(+)/H(+) exchange regulatory cofactor 3 (NHERF3), which plays a critical role in cholesterol metabolism and carnitine transport, was found in the lipid metabolism. Western blotting and immunofluorescence analyses showed that Pdzk1 was expressed in the epididymis but not in the testis, and localized at the middle piece of the sperm tail. Pdzk1 protein level was also reduced in the spermatozoa in case of asthenozoospermic patients compared with that in normozoospermic men, suggesting that Pdzk1 may participate in sperm maturation regulation and may be associated with male infertility. These results may provide new insights into the mechanisms of sperm maturation and male infertility. Medknow Publications & Media Pvt Ltd 2018 2018-01-19 /pmc/articles/PMC5858100/ /pubmed/29405165 http://dx.doi.org/10.4103/aja.aja_65_17 Text en Copyright: © The Author(s)(2018) http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 3.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as the author is credited and the new creations are licensed under the identical terms.
spellingShingle Original Article
Liang, A-Juan
Wang, Gui-Shuan
Ping, Ping
Hu, Shuang-Gang
Lin, Yu
Ma, Yi
Duan, Zheng-Zheng
Wang, Han-Shu
Sun, Fei
The expression of the new epididymal luminal protein of PDZ domain containing 1 is decreased in asthenozoospermia
title The expression of the new epididymal luminal protein of PDZ domain containing 1 is decreased in asthenozoospermia
title_full The expression of the new epididymal luminal protein of PDZ domain containing 1 is decreased in asthenozoospermia
title_fullStr The expression of the new epididymal luminal protein of PDZ domain containing 1 is decreased in asthenozoospermia
title_full_unstemmed The expression of the new epididymal luminal protein of PDZ domain containing 1 is decreased in asthenozoospermia
title_short The expression of the new epididymal luminal protein of PDZ domain containing 1 is decreased in asthenozoospermia
title_sort expression of the new epididymal luminal protein of pdz domain containing 1 is decreased in asthenozoospermia
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5858100/
https://www.ncbi.nlm.nih.gov/pubmed/29405165
http://dx.doi.org/10.4103/aja.aja_65_17
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