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The role of PTRF/Cavin1 as a biomarker in both glioma and serum exosomes
Exosomes play critical roles in intercellular communication in both nearby and distant cells in individuals and organs. Polymerase I and transcript release factor (PTRF), also known as Cavin1, has previously been described as a critical factor in caveola formation, and aberrant PTRF expression has b...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Ivyspring International Publisher
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5858166/ https://www.ncbi.nlm.nih.gov/pubmed/29556340 http://dx.doi.org/10.7150/thno.22952 |
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author | Huang, Kai Fang, Chuan Yi, Kaikai Liu, Xing Qi, Hongzhao Tan, Yanli Zhou, Junhu Li, Ying Liu, Mingyang Zhang, Yuqing Yang, Jingxuan Zhang, Jianning Li, Min Kang, Chunsheng |
author_facet | Huang, Kai Fang, Chuan Yi, Kaikai Liu, Xing Qi, Hongzhao Tan, Yanli Zhou, Junhu Li, Ying Liu, Mingyang Zhang, Yuqing Yang, Jingxuan Zhang, Jianning Li, Min Kang, Chunsheng |
author_sort | Huang, Kai |
collection | PubMed |
description | Exosomes play critical roles in intercellular communication in both nearby and distant cells in individuals and organs. Polymerase I and transcript release factor (PTRF), also known as Cavin1, has previously been described as a critical factor in caveola formation, and aberrant PTRF expression has been reported in various malignancies. However, the function of PTRF in tumor progression remains controversial, and its role in glioma is poorly understood. In this study, we report that PTRF is associated with malignancy grade and poor prognosis in glioma patients. Our previous study using two proteomics methods, tandem mass tag (TMT) and data-independent acquisition (DIA), showed that EGFRvIII overexpression increased PTRF expression at the protein level. In contrast, blocking PI3K and AKT using LY294002 and MK-2206, respectively, decreased PTRF expression, showing that PTRF is regulated in the EGFR/PI3K/AKT pathway. ChIP-PCR analysis showed that PTRF is transcriptionally regulated by the H3K4me3 and H3K27me3 modifications. Furthermore, PTRF overexpression increased exosome secretion and induced cell growth in vitro. More importantly, overexpressing PTRF induced the malignancy of nearby cells in vivo, suggesting that PTRF alters the microenvironment through intercellular communication via exosomes. Furthermore, analysis of clinical samples showed a positive correlation between tumor grade and PTRF expression in both tumor tissues and exosomes isolated from blood harvested from glioma patients, and PTRF expression in exosomes isolated from the sera of GBM patients was decreased after surgery. In conclusion, PTRF serves as a promising biomarker in both tumor samples and serum exosomes, thus facilitating the detection of glioma and potentially serving as a therapeutic target for glioblastoma multiforme. |
format | Online Article Text |
id | pubmed-5858166 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Ivyspring International Publisher |
record_format | MEDLINE/PubMed |
spelling | pubmed-58581662018-03-19 The role of PTRF/Cavin1 as a biomarker in both glioma and serum exosomes Huang, Kai Fang, Chuan Yi, Kaikai Liu, Xing Qi, Hongzhao Tan, Yanli Zhou, Junhu Li, Ying Liu, Mingyang Zhang, Yuqing Yang, Jingxuan Zhang, Jianning Li, Min Kang, Chunsheng Theranostics Research Paper Exosomes play critical roles in intercellular communication in both nearby and distant cells in individuals and organs. Polymerase I and transcript release factor (PTRF), also known as Cavin1, has previously been described as a critical factor in caveola formation, and aberrant PTRF expression has been reported in various malignancies. However, the function of PTRF in tumor progression remains controversial, and its role in glioma is poorly understood. In this study, we report that PTRF is associated with malignancy grade and poor prognosis in glioma patients. Our previous study using two proteomics methods, tandem mass tag (TMT) and data-independent acquisition (DIA), showed that EGFRvIII overexpression increased PTRF expression at the protein level. In contrast, blocking PI3K and AKT using LY294002 and MK-2206, respectively, decreased PTRF expression, showing that PTRF is regulated in the EGFR/PI3K/AKT pathway. ChIP-PCR analysis showed that PTRF is transcriptionally regulated by the H3K4me3 and H3K27me3 modifications. Furthermore, PTRF overexpression increased exosome secretion and induced cell growth in vitro. More importantly, overexpressing PTRF induced the malignancy of nearby cells in vivo, suggesting that PTRF alters the microenvironment through intercellular communication via exosomes. Furthermore, analysis of clinical samples showed a positive correlation between tumor grade and PTRF expression in both tumor tissues and exosomes isolated from blood harvested from glioma patients, and PTRF expression in exosomes isolated from the sera of GBM patients was decreased after surgery. In conclusion, PTRF serves as a promising biomarker in both tumor samples and serum exosomes, thus facilitating the detection of glioma and potentially serving as a therapeutic target for glioblastoma multiforme. Ivyspring International Publisher 2018-02-07 /pmc/articles/PMC5858166/ /pubmed/29556340 http://dx.doi.org/10.7150/thno.22952 Text en © Ivyspring International Publisher This is an open access article distributed under the terms of the Creative Commons Attribution (CC BY-NC) license (https://creativecommons.org/licenses/by-nc/4.0/). See http://ivyspring.com/terms for full terms and conditions. |
spellingShingle | Research Paper Huang, Kai Fang, Chuan Yi, Kaikai Liu, Xing Qi, Hongzhao Tan, Yanli Zhou, Junhu Li, Ying Liu, Mingyang Zhang, Yuqing Yang, Jingxuan Zhang, Jianning Li, Min Kang, Chunsheng The role of PTRF/Cavin1 as a biomarker in both glioma and serum exosomes |
title | The role of PTRF/Cavin1 as a biomarker in both glioma and serum exosomes |
title_full | The role of PTRF/Cavin1 as a biomarker in both glioma and serum exosomes |
title_fullStr | The role of PTRF/Cavin1 as a biomarker in both glioma and serum exosomes |
title_full_unstemmed | The role of PTRF/Cavin1 as a biomarker in both glioma and serum exosomes |
title_short | The role of PTRF/Cavin1 as a biomarker in both glioma and serum exosomes |
title_sort | role of ptrf/cavin1 as a biomarker in both glioma and serum exosomes |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5858166/ https://www.ncbi.nlm.nih.gov/pubmed/29556340 http://dx.doi.org/10.7150/thno.22952 |
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