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Near-Patient Sampling to Assist Infection Control—A Case Report and Discussion

Air sampling as an aid to infection control is still in an experimental stage, as there is no consensus about which air samplers and pathogen detection methods should be used, and what thresholds of specific pathogens in specific exposed populations (staff, patients, or visitors) constitutes a true...

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Autores principales: Tang, Julian W., Hoyle, Elizabeth, Moran, Sammy, Pareek, Manish
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5858307/
https://www.ncbi.nlm.nih.gov/pubmed/29385031
http://dx.doi.org/10.3390/ijerph15020238
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author Tang, Julian W.
Hoyle, Elizabeth
Moran, Sammy
Pareek, Manish
author_facet Tang, Julian W.
Hoyle, Elizabeth
Moran, Sammy
Pareek, Manish
author_sort Tang, Julian W.
collection PubMed
description Air sampling as an aid to infection control is still in an experimental stage, as there is no consensus about which air samplers and pathogen detection methods should be used, and what thresholds of specific pathogens in specific exposed populations (staff, patients, or visitors) constitutes a true clinical risk. This case report used a button sampler, worn or held by staff or left free-standing in a fixed location, for environmental sampling around a child who was chronically infected by a respiratory adenovirus, to determine whether there was any risk of secondary adenovirus infection to the staff managing the patient. Despite multiple air samples taken on difference days, coinciding with high levels of adenovirus detectable in the child’s nasopharyngeal aspirates (NPAs), none of the air samples contained any detectable adenovirus DNA using a clinically validated diagnostic polymerase chain reaction (PCR) assay. Although highly sensitive, in-house PCR assays have been developed to detect airborne pathogen RNA/DNA, it is still unclear what level of specific pathogen RNA/DNA constitutes a true clinical risk. In this case, the absence of detectable airborne adenovirus DNA using a conventional diagnostic assay removed the requirement for staff to wear surgical masks and face visors when they entered the child’s room. No subsequent staff infections or outbreaks of adenovirus have so far been identified.
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spelling pubmed-58583072018-03-19 Near-Patient Sampling to Assist Infection Control—A Case Report and Discussion Tang, Julian W. Hoyle, Elizabeth Moran, Sammy Pareek, Manish Int J Environ Res Public Health Case Report Air sampling as an aid to infection control is still in an experimental stage, as there is no consensus about which air samplers and pathogen detection methods should be used, and what thresholds of specific pathogens in specific exposed populations (staff, patients, or visitors) constitutes a true clinical risk. This case report used a button sampler, worn or held by staff or left free-standing in a fixed location, for environmental sampling around a child who was chronically infected by a respiratory adenovirus, to determine whether there was any risk of secondary adenovirus infection to the staff managing the patient. Despite multiple air samples taken on difference days, coinciding with high levels of adenovirus detectable in the child’s nasopharyngeal aspirates (NPAs), none of the air samples contained any detectable adenovirus DNA using a clinically validated diagnostic polymerase chain reaction (PCR) assay. Although highly sensitive, in-house PCR assays have been developed to detect airborne pathogen RNA/DNA, it is still unclear what level of specific pathogen RNA/DNA constitutes a true clinical risk. In this case, the absence of detectable airborne adenovirus DNA using a conventional diagnostic assay removed the requirement for staff to wear surgical masks and face visors when they entered the child’s room. No subsequent staff infections or outbreaks of adenovirus have so far been identified. MDPI 2018-01-31 2018-02 /pmc/articles/PMC5858307/ /pubmed/29385031 http://dx.doi.org/10.3390/ijerph15020238 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Case Report
Tang, Julian W.
Hoyle, Elizabeth
Moran, Sammy
Pareek, Manish
Near-Patient Sampling to Assist Infection Control—A Case Report and Discussion
title Near-Patient Sampling to Assist Infection Control—A Case Report and Discussion
title_full Near-Patient Sampling to Assist Infection Control—A Case Report and Discussion
title_fullStr Near-Patient Sampling to Assist Infection Control—A Case Report and Discussion
title_full_unstemmed Near-Patient Sampling to Assist Infection Control—A Case Report and Discussion
title_short Near-Patient Sampling to Assist Infection Control—A Case Report and Discussion
title_sort near-patient sampling to assist infection control—a case report and discussion
topic Case Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5858307/
https://www.ncbi.nlm.nih.gov/pubmed/29385031
http://dx.doi.org/10.3390/ijerph15020238
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