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Zinc oxide and silver nanoparticles toxicity in the baker's yeast, Saccharomyces cerevisiae

Engineered nanomaterials (ENMs) are increasingly incorporated into a variety of commercial applications and consumer products; however, ENMs may possess cytotoxic properties due to their small size. This study assessed the effects of two commonly used ENMs, zinc oxide nanoparticles (ZnONPs) and silv...

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Autores principales: Galván Márquez, Imelda, Ghiyasvand, Mergan, Massarsky, Andrey, Babu, Mohan, Samanfar, Bahram, Omidi, Katayoun, Moon, Thomas W., Smith, Myron L., Golshani, Ashkan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5858749/
https://www.ncbi.nlm.nih.gov/pubmed/29554091
http://dx.doi.org/10.1371/journal.pone.0193111
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author Galván Márquez, Imelda
Ghiyasvand, Mergan
Massarsky, Andrey
Babu, Mohan
Samanfar, Bahram
Omidi, Katayoun
Moon, Thomas W.
Smith, Myron L.
Golshani, Ashkan
author_facet Galván Márquez, Imelda
Ghiyasvand, Mergan
Massarsky, Andrey
Babu, Mohan
Samanfar, Bahram
Omidi, Katayoun
Moon, Thomas W.
Smith, Myron L.
Golshani, Ashkan
author_sort Galván Márquez, Imelda
collection PubMed
description Engineered nanomaterials (ENMs) are increasingly incorporated into a variety of commercial applications and consumer products; however, ENMs may possess cytotoxic properties due to their small size. This study assessed the effects of two commonly used ENMs, zinc oxide nanoparticles (ZnONPs) and silver nanoparticles (AgNPs), in the model eukaryote Saccharomyces cerevisiae. A collection of ≈4600 S. cerevisiae deletion mutant strains was used to deduce the genes, whose absence makes S. cerevisiae more prone to the cytotoxic effects of ZnONPs or AgNPs. We demonstrate that S. cerevisiae strains that lack genes involved in transmembrane and membrane transport, cellular ion homeostasis, and cell wall organization or biogenesis exhibited the highest sensitivity to ZnONPs. In contrast, strains that lack genes involved in transcription and RNA processing, cellular respiration, and endocytosis and vesicular transport exhibited the highest sensitivity to AgNPs. Secondary assays confirmed that ZnONPs affected cell wall function and integrity, whereas AgNPs exposure decreased transcription, reduced endocytosis, and led to a dysfunctional electron transport system. This study supports the use of S. cerevisiae Gene Deletion Array as an effective high-throughput technique to determine cellular targets of ENM toxicity.
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spelling pubmed-58587492018-03-28 Zinc oxide and silver nanoparticles toxicity in the baker's yeast, Saccharomyces cerevisiae Galván Márquez, Imelda Ghiyasvand, Mergan Massarsky, Andrey Babu, Mohan Samanfar, Bahram Omidi, Katayoun Moon, Thomas W. Smith, Myron L. Golshani, Ashkan PLoS One Research Article Engineered nanomaterials (ENMs) are increasingly incorporated into a variety of commercial applications and consumer products; however, ENMs may possess cytotoxic properties due to their small size. This study assessed the effects of two commonly used ENMs, zinc oxide nanoparticles (ZnONPs) and silver nanoparticles (AgNPs), in the model eukaryote Saccharomyces cerevisiae. A collection of ≈4600 S. cerevisiae deletion mutant strains was used to deduce the genes, whose absence makes S. cerevisiae more prone to the cytotoxic effects of ZnONPs or AgNPs. We demonstrate that S. cerevisiae strains that lack genes involved in transmembrane and membrane transport, cellular ion homeostasis, and cell wall organization or biogenesis exhibited the highest sensitivity to ZnONPs. In contrast, strains that lack genes involved in transcription and RNA processing, cellular respiration, and endocytosis and vesicular transport exhibited the highest sensitivity to AgNPs. Secondary assays confirmed that ZnONPs affected cell wall function and integrity, whereas AgNPs exposure decreased transcription, reduced endocytosis, and led to a dysfunctional electron transport system. This study supports the use of S. cerevisiae Gene Deletion Array as an effective high-throughput technique to determine cellular targets of ENM toxicity. Public Library of Science 2018-03-19 /pmc/articles/PMC5858749/ /pubmed/29554091 http://dx.doi.org/10.1371/journal.pone.0193111 Text en © 2018 Galván Márquez et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Galván Márquez, Imelda
Ghiyasvand, Mergan
Massarsky, Andrey
Babu, Mohan
Samanfar, Bahram
Omidi, Katayoun
Moon, Thomas W.
Smith, Myron L.
Golshani, Ashkan
Zinc oxide and silver nanoparticles toxicity in the baker's yeast, Saccharomyces cerevisiae
title Zinc oxide and silver nanoparticles toxicity in the baker's yeast, Saccharomyces cerevisiae
title_full Zinc oxide and silver nanoparticles toxicity in the baker's yeast, Saccharomyces cerevisiae
title_fullStr Zinc oxide and silver nanoparticles toxicity in the baker's yeast, Saccharomyces cerevisiae
title_full_unstemmed Zinc oxide and silver nanoparticles toxicity in the baker's yeast, Saccharomyces cerevisiae
title_short Zinc oxide and silver nanoparticles toxicity in the baker's yeast, Saccharomyces cerevisiae
title_sort zinc oxide and silver nanoparticles toxicity in the baker's yeast, saccharomyces cerevisiae
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5858749/
https://www.ncbi.nlm.nih.gov/pubmed/29554091
http://dx.doi.org/10.1371/journal.pone.0193111
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