Cargando…
Functional and evolutionary implications from the molecular characterization of five spermatophore CHH/MIH/GIH genes in the shrimp Fenneropenaeus merguiensis
The recent use of RNA-Seq to study the transcriptomes of different species has helped identify a large number of new genes from different non-model organisms. In this study, five distinctive transcripts encoding for neuropeptide members of the CHH/MIH/GIH family have been identified from the spermat...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2018
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5858750/ https://www.ncbi.nlm.nih.gov/pubmed/29554093 http://dx.doi.org/10.1371/journal.pone.0193375 |
_version_ | 1783307706272055296 |
---|---|
author | Shi, LiLi Li, Bin Zhou, Ting Ting Wang, Wei Chan, Siuming F. |
author_facet | Shi, LiLi Li, Bin Zhou, Ting Ting Wang, Wei Chan, Siuming F. |
author_sort | Shi, LiLi |
collection | PubMed |
description | The recent use of RNA-Seq to study the transcriptomes of different species has helped identify a large number of new genes from different non-model organisms. In this study, five distinctive transcripts encoding for neuropeptide members of the CHH/MIH/GIH family have been identified from the spermatophore transcriptome of the shrimp Fenneropenaeus merguiensis. The size of these transcripts ranged from 531 bp to 1771 bp. Four transcripts encoded different CHH-family subtype I members, and one transcript encoded a subtype II member. RT-PCR and RACE approaches have confirmed the expression of these genes in males. The low degree of amino acid sequence identity among these neuropeptides suggests that they may have different specific function(s). Results from a phylogenetic tree analysis indicated that these neuropeptides were likely derived from a common ancestor gene resulting from mutation and gene duplication. These CHH-family members could be grouped into distinct clusters, indicating a strong structural/functional relationship among these neuropeptides. Eyestalk removal caused a significant increase in the expression of transcript 32710 but decreases in expression for transcript 28020. These findings suggest the possible regulation of these genes by eyestalk factor(s). In summary, the results of this study would justify a re-evaluation of the more generalized and pleiotropic functions of these neuropeptides. This study also represents the first report on the cloning/identification of five CHH family neuropeptides in a non-neuronal tissue from a single crustacean species. |
format | Online Article Text |
id | pubmed-5858750 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-58587502018-03-28 Functional and evolutionary implications from the molecular characterization of five spermatophore CHH/MIH/GIH genes in the shrimp Fenneropenaeus merguiensis Shi, LiLi Li, Bin Zhou, Ting Ting Wang, Wei Chan, Siuming F. PLoS One Research Article The recent use of RNA-Seq to study the transcriptomes of different species has helped identify a large number of new genes from different non-model organisms. In this study, five distinctive transcripts encoding for neuropeptide members of the CHH/MIH/GIH family have been identified from the spermatophore transcriptome of the shrimp Fenneropenaeus merguiensis. The size of these transcripts ranged from 531 bp to 1771 bp. Four transcripts encoded different CHH-family subtype I members, and one transcript encoded a subtype II member. RT-PCR and RACE approaches have confirmed the expression of these genes in males. The low degree of amino acid sequence identity among these neuropeptides suggests that they may have different specific function(s). Results from a phylogenetic tree analysis indicated that these neuropeptides were likely derived from a common ancestor gene resulting from mutation and gene duplication. These CHH-family members could be grouped into distinct clusters, indicating a strong structural/functional relationship among these neuropeptides. Eyestalk removal caused a significant increase in the expression of transcript 32710 but decreases in expression for transcript 28020. These findings suggest the possible regulation of these genes by eyestalk factor(s). In summary, the results of this study would justify a re-evaluation of the more generalized and pleiotropic functions of these neuropeptides. This study also represents the first report on the cloning/identification of five CHH family neuropeptides in a non-neuronal tissue from a single crustacean species. Public Library of Science 2018-03-19 /pmc/articles/PMC5858750/ /pubmed/29554093 http://dx.doi.org/10.1371/journal.pone.0193375 Text en © 2018 Shi et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Shi, LiLi Li, Bin Zhou, Ting Ting Wang, Wei Chan, Siuming F. Functional and evolutionary implications from the molecular characterization of five spermatophore CHH/MIH/GIH genes in the shrimp Fenneropenaeus merguiensis |
title | Functional and evolutionary implications from the molecular characterization of five spermatophore CHH/MIH/GIH genes in the shrimp Fenneropenaeus merguiensis |
title_full | Functional and evolutionary implications from the molecular characterization of five spermatophore CHH/MIH/GIH genes in the shrimp Fenneropenaeus merguiensis |
title_fullStr | Functional and evolutionary implications from the molecular characterization of five spermatophore CHH/MIH/GIH genes in the shrimp Fenneropenaeus merguiensis |
title_full_unstemmed | Functional and evolutionary implications from the molecular characterization of five spermatophore CHH/MIH/GIH genes in the shrimp Fenneropenaeus merguiensis |
title_short | Functional and evolutionary implications from the molecular characterization of five spermatophore CHH/MIH/GIH genes in the shrimp Fenneropenaeus merguiensis |
title_sort | functional and evolutionary implications from the molecular characterization of five spermatophore chh/mih/gih genes in the shrimp fenneropenaeus merguiensis |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5858750/ https://www.ncbi.nlm.nih.gov/pubmed/29554093 http://dx.doi.org/10.1371/journal.pone.0193375 |
work_keys_str_mv | AT shilili functionalandevolutionaryimplicationsfromthemolecularcharacterizationoffivespermatophorechhmihgihgenesintheshrimpfenneropenaeusmerguiensis AT libin functionalandevolutionaryimplicationsfromthemolecularcharacterizationoffivespermatophorechhmihgihgenesintheshrimpfenneropenaeusmerguiensis AT zhoutingting functionalandevolutionaryimplicationsfromthemolecularcharacterizationoffivespermatophorechhmihgihgenesintheshrimpfenneropenaeusmerguiensis AT wangwei functionalandevolutionaryimplicationsfromthemolecularcharacterizationoffivespermatophorechhmihgihgenesintheshrimpfenneropenaeusmerguiensis AT chansiumingf functionalandevolutionaryimplicationsfromthemolecularcharacterizationoffivespermatophorechhmihgihgenesintheshrimpfenneropenaeusmerguiensis |