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Effect of dietary restriction and subsequent re-alimentation on the transcriptional profile of bovine jejunal epithelium

Compensatory growth (CG), an accelerated growth phenomenon which occurs following a period of dietary restriction is utilised worldwide in animal production systems as a management practise to lower feed costs. The objective of this study was to evaluate the contribution of jejunal epithelial to CG...

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Autores principales: Keogh, Kate, Waters, Sinead M., Cormican, Paul, Kelly, Alan K., Kenny, David A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5858768/
https://www.ncbi.nlm.nih.gov/pubmed/29554113
http://dx.doi.org/10.1371/journal.pone.0194445
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author Keogh, Kate
Waters, Sinead M.
Cormican, Paul
Kelly, Alan K.
Kenny, David A.
author_facet Keogh, Kate
Waters, Sinead M.
Cormican, Paul
Kelly, Alan K.
Kenny, David A.
author_sort Keogh, Kate
collection PubMed
description Compensatory growth (CG), an accelerated growth phenomenon which occurs following a period of dietary restriction is utilised worldwide in animal production systems as a management practise to lower feed costs. The objective of this study was to evaluate the contribution of jejunal epithelial to CG in cattle through transcriptional profiling following a period of dietary restriction as well as subsequent re-alimentation induced CG. Sixty Holstein Friesian bulls were separated into two groups; RES and ADLIB, with 30 animals in each. RES animals were offered a restricted diet for 125 days (Period 1) followed by ad libitum feeding for 55 days (Period 2). ADLIB animals had ad libitum access to feed across both periods 1 and 2. At the end of each period, 15 animals from each treatment group were slaughtered, jejunal epithelium collected and RNAseq analysis performed. Animals that were previously diet restricted underwent CG, gaining 1.8 times the rate of their non-restricted counterparts. Twenty-four genes were differentially expressed in RES compared to ADLIB animals at the end of Period 1, with only one gene, GSTA1, differentially expressed between the two groups at the end of Period 2. When analysed within treatment (RES, Period 2 v Period 1), 31 genes were differentially expressed between diet restricted and animals undergoing CG. Dietary restriction and subsequent re-alimentation were associated with altered expression of genes involved in digestion and metabolism as well as those involved in cellular division and growth. Compensatory growth was also associated with greater expression of genes involved in cellular protection and detoxification in jejunal epithelium. This study highlights some of the molecular mechanisms regulating the response to dietary restriction and subsequent re-alimentation induced CG in cattle; however the gene expression results suggest that most of the CG in jejunal epithelium had occurred by day 55 of re-alimentation.
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spelling pubmed-58587682018-03-28 Effect of dietary restriction and subsequent re-alimentation on the transcriptional profile of bovine jejunal epithelium Keogh, Kate Waters, Sinead M. Cormican, Paul Kelly, Alan K. Kenny, David A. PLoS One Research Article Compensatory growth (CG), an accelerated growth phenomenon which occurs following a period of dietary restriction is utilised worldwide in animal production systems as a management practise to lower feed costs. The objective of this study was to evaluate the contribution of jejunal epithelial to CG in cattle through transcriptional profiling following a period of dietary restriction as well as subsequent re-alimentation induced CG. Sixty Holstein Friesian bulls were separated into two groups; RES and ADLIB, with 30 animals in each. RES animals were offered a restricted diet for 125 days (Period 1) followed by ad libitum feeding for 55 days (Period 2). ADLIB animals had ad libitum access to feed across both periods 1 and 2. At the end of each period, 15 animals from each treatment group were slaughtered, jejunal epithelium collected and RNAseq analysis performed. Animals that were previously diet restricted underwent CG, gaining 1.8 times the rate of their non-restricted counterparts. Twenty-four genes were differentially expressed in RES compared to ADLIB animals at the end of Period 1, with only one gene, GSTA1, differentially expressed between the two groups at the end of Period 2. When analysed within treatment (RES, Period 2 v Period 1), 31 genes were differentially expressed between diet restricted and animals undergoing CG. Dietary restriction and subsequent re-alimentation were associated with altered expression of genes involved in digestion and metabolism as well as those involved in cellular division and growth. Compensatory growth was also associated with greater expression of genes involved in cellular protection and detoxification in jejunal epithelium. This study highlights some of the molecular mechanisms regulating the response to dietary restriction and subsequent re-alimentation induced CG in cattle; however the gene expression results suggest that most of the CG in jejunal epithelium had occurred by day 55 of re-alimentation. Public Library of Science 2018-03-19 /pmc/articles/PMC5858768/ /pubmed/29554113 http://dx.doi.org/10.1371/journal.pone.0194445 Text en © 2018 Keogh et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Keogh, Kate
Waters, Sinead M.
Cormican, Paul
Kelly, Alan K.
Kenny, David A.
Effect of dietary restriction and subsequent re-alimentation on the transcriptional profile of bovine jejunal epithelium
title Effect of dietary restriction and subsequent re-alimentation on the transcriptional profile of bovine jejunal epithelium
title_full Effect of dietary restriction and subsequent re-alimentation on the transcriptional profile of bovine jejunal epithelium
title_fullStr Effect of dietary restriction and subsequent re-alimentation on the transcriptional profile of bovine jejunal epithelium
title_full_unstemmed Effect of dietary restriction and subsequent re-alimentation on the transcriptional profile of bovine jejunal epithelium
title_short Effect of dietary restriction and subsequent re-alimentation on the transcriptional profile of bovine jejunal epithelium
title_sort effect of dietary restriction and subsequent re-alimentation on the transcriptional profile of bovine jejunal epithelium
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5858768/
https://www.ncbi.nlm.nih.gov/pubmed/29554113
http://dx.doi.org/10.1371/journal.pone.0194445
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