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The effect of storage delay and storage temperature on orthopaedic surgical samples contaminated by Staphylococcus Epidermidis
BACKGROUND: Prosthetic Joint Infection (PJI) is a rare but devastating complications with high morbitity and mortality. The identification of the causal microorganism remains crucial and determines therapeutic strategies and success. Microbiology cultures remain the common method to diagnose PJI. Un...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5858844/ https://www.ncbi.nlm.nih.gov/pubmed/29554102 http://dx.doi.org/10.1371/journal.pone.0192048 |
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author | Van Cauter, Maïté Cornu, Olivier Yombi, Jean-Cyr Rodriguez-Villalobos, Hector Kaminski, Ludovic |
author_facet | Van Cauter, Maïté Cornu, Olivier Yombi, Jean-Cyr Rodriguez-Villalobos, Hector Kaminski, Ludovic |
author_sort | Van Cauter, Maïté |
collection | PubMed |
description | BACKGROUND: Prosthetic Joint Infection (PJI) is a rare but devastating complications with high morbitity and mortality. The identification of the causal microorganism remains crucial and determines therapeutic strategies and success. Microbiology cultures remain the common method to diagnose PJI. Unfortunately, 14% of intra-articular punctures remain negative after culture. The microorganisms are best detected by inoculation of microbiology samples in blood culture bottles (Bactec), or after sonication of the implant and polymerase chain reaction (PCR). The identification of the causal microorganism remains crucial and determines therapeutic success. OBJECTIVES: This study was conducted to assess the effect of culture lead time and sample storage temperature on the detection of the pathogen. METHODS: We obtained bone fragments from femoral heads during primary arthroplasty. Bone fragments were contaminated with a strain of Staphylococcus epidermidis. Four set-ups with different combinations of storage delay and storage temperature were tested. RESULTS: Our study shows the need to cultivate as soon as possible and optimally within 2h after the completion of sampling. Temporary storage in a refrigerator at 4°C also appears to have a positive influence on bacterial viability. At present, these conclusions concern only the Staphylococcus Epidermidis. Others studies are requested to generalize this conclusion to other bacteria. |
format | Online Article Text |
id | pubmed-5858844 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-58588442018-03-28 The effect of storage delay and storage temperature on orthopaedic surgical samples contaminated by Staphylococcus Epidermidis Van Cauter, Maïté Cornu, Olivier Yombi, Jean-Cyr Rodriguez-Villalobos, Hector Kaminski, Ludovic PLoS One Research Article BACKGROUND: Prosthetic Joint Infection (PJI) is a rare but devastating complications with high morbitity and mortality. The identification of the causal microorganism remains crucial and determines therapeutic strategies and success. Microbiology cultures remain the common method to diagnose PJI. Unfortunately, 14% of intra-articular punctures remain negative after culture. The microorganisms are best detected by inoculation of microbiology samples in blood culture bottles (Bactec), or after sonication of the implant and polymerase chain reaction (PCR). The identification of the causal microorganism remains crucial and determines therapeutic success. OBJECTIVES: This study was conducted to assess the effect of culture lead time and sample storage temperature on the detection of the pathogen. METHODS: We obtained bone fragments from femoral heads during primary arthroplasty. Bone fragments were contaminated with a strain of Staphylococcus epidermidis. Four set-ups with different combinations of storage delay and storage temperature were tested. RESULTS: Our study shows the need to cultivate as soon as possible and optimally within 2h after the completion of sampling. Temporary storage in a refrigerator at 4°C also appears to have a positive influence on bacterial viability. At present, these conclusions concern only the Staphylococcus Epidermidis. Others studies are requested to generalize this conclusion to other bacteria. Public Library of Science 2018-03-19 /pmc/articles/PMC5858844/ /pubmed/29554102 http://dx.doi.org/10.1371/journal.pone.0192048 Text en © 2018 Van Cauter et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Van Cauter, Maïté Cornu, Olivier Yombi, Jean-Cyr Rodriguez-Villalobos, Hector Kaminski, Ludovic The effect of storage delay and storage temperature on orthopaedic surgical samples contaminated by Staphylococcus Epidermidis |
title | The effect of storage delay and storage temperature on orthopaedic surgical samples contaminated by Staphylococcus Epidermidis |
title_full | The effect of storage delay and storage temperature on orthopaedic surgical samples contaminated by Staphylococcus Epidermidis |
title_fullStr | The effect of storage delay and storage temperature on orthopaedic surgical samples contaminated by Staphylococcus Epidermidis |
title_full_unstemmed | The effect of storage delay and storage temperature on orthopaedic surgical samples contaminated by Staphylococcus Epidermidis |
title_short | The effect of storage delay and storage temperature on orthopaedic surgical samples contaminated by Staphylococcus Epidermidis |
title_sort | effect of storage delay and storage temperature on orthopaedic surgical samples contaminated by staphylococcus epidermidis |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5858844/ https://www.ncbi.nlm.nih.gov/pubmed/29554102 http://dx.doi.org/10.1371/journal.pone.0192048 |
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