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Revisiting Francisella tularensis subsp. holarctica, Causative Agent of Tularemia in Germany With Bioinformatics: New Insights in Genome Structure, DNA Methylation and Comparative Phylogenetic Analysis

Francisella (F.) tularensis is a highly virulent, Gram-negative bacterial pathogen and the causative agent of the zoonotic disease tularemia. Here, we generated, analyzed and characterized a high quality circular genome sequence of the F. tularensis subsp. holarctica strain 12T0050 that caused fatal...

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Autores principales: Busch, Anne, Thomas, Prasad, Zuchantke, Eric, Brendebach, Holger, Neubert, Kerstin, Gruetzke, Josephine, Al Dahouk, Sascha, Peters, Martin, Hotzel, Helmut, Neubauer, Heinrich, Tomaso, Herbert
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5859110/
https://www.ncbi.nlm.nih.gov/pubmed/29593661
http://dx.doi.org/10.3389/fmicb.2018.00344
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author Busch, Anne
Thomas, Prasad
Zuchantke, Eric
Brendebach, Holger
Neubert, Kerstin
Gruetzke, Josephine
Al Dahouk, Sascha
Peters, Martin
Hotzel, Helmut
Neubauer, Heinrich
Tomaso, Herbert
author_facet Busch, Anne
Thomas, Prasad
Zuchantke, Eric
Brendebach, Holger
Neubert, Kerstin
Gruetzke, Josephine
Al Dahouk, Sascha
Peters, Martin
Hotzel, Helmut
Neubauer, Heinrich
Tomaso, Herbert
author_sort Busch, Anne
collection PubMed
description Francisella (F.) tularensis is a highly virulent, Gram-negative bacterial pathogen and the causative agent of the zoonotic disease tularemia. Here, we generated, analyzed and characterized a high quality circular genome sequence of the F. tularensis subsp. holarctica strain 12T0050 that caused fatal tularemia in a hare. Besides the genomic structure, we focused on the analysis of oriC, unique to the Francisella genus and regulating replication in and outside hosts and the first report on genomic DNA methylation of a Francisella strain. The high quality genome was used to establish and evaluate a diagnostic whole genome sequencing pipeline. A genotyping strategy for F. tularensis was developed using various bioinformatics tools for genotyping. Additionally, whole genome sequences of F. tularensis subsp. holarctica isolates isolated in the years 2008–2015 in Germany were generated. A phylogenetic analysis allowed to determine the genetic relatedness of these isolates and confirmed the highly conserved nature of F. tularensis subsp. holarctica.
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spelling pubmed-58591102018-03-28 Revisiting Francisella tularensis subsp. holarctica, Causative Agent of Tularemia in Germany With Bioinformatics: New Insights in Genome Structure, DNA Methylation and Comparative Phylogenetic Analysis Busch, Anne Thomas, Prasad Zuchantke, Eric Brendebach, Holger Neubert, Kerstin Gruetzke, Josephine Al Dahouk, Sascha Peters, Martin Hotzel, Helmut Neubauer, Heinrich Tomaso, Herbert Front Microbiol Microbiology Francisella (F.) tularensis is a highly virulent, Gram-negative bacterial pathogen and the causative agent of the zoonotic disease tularemia. Here, we generated, analyzed and characterized a high quality circular genome sequence of the F. tularensis subsp. holarctica strain 12T0050 that caused fatal tularemia in a hare. Besides the genomic structure, we focused on the analysis of oriC, unique to the Francisella genus and regulating replication in and outside hosts and the first report on genomic DNA methylation of a Francisella strain. The high quality genome was used to establish and evaluate a diagnostic whole genome sequencing pipeline. A genotyping strategy for F. tularensis was developed using various bioinformatics tools for genotyping. Additionally, whole genome sequences of F. tularensis subsp. holarctica isolates isolated in the years 2008–2015 in Germany were generated. A phylogenetic analysis allowed to determine the genetic relatedness of these isolates and confirmed the highly conserved nature of F. tularensis subsp. holarctica. Frontiers Media S.A. 2018-03-13 /pmc/articles/PMC5859110/ /pubmed/29593661 http://dx.doi.org/10.3389/fmicb.2018.00344 Text en Copyright © 2018 Busch, Thomas, Zuchantke, Brendebach, Neubert, Gruetzke, Al Dahouk, Peters, Hotzel, Neubauer and Tomaso. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Busch, Anne
Thomas, Prasad
Zuchantke, Eric
Brendebach, Holger
Neubert, Kerstin
Gruetzke, Josephine
Al Dahouk, Sascha
Peters, Martin
Hotzel, Helmut
Neubauer, Heinrich
Tomaso, Herbert
Revisiting Francisella tularensis subsp. holarctica, Causative Agent of Tularemia in Germany With Bioinformatics: New Insights in Genome Structure, DNA Methylation and Comparative Phylogenetic Analysis
title Revisiting Francisella tularensis subsp. holarctica, Causative Agent of Tularemia in Germany With Bioinformatics: New Insights in Genome Structure, DNA Methylation and Comparative Phylogenetic Analysis
title_full Revisiting Francisella tularensis subsp. holarctica, Causative Agent of Tularemia in Germany With Bioinformatics: New Insights in Genome Structure, DNA Methylation and Comparative Phylogenetic Analysis
title_fullStr Revisiting Francisella tularensis subsp. holarctica, Causative Agent of Tularemia in Germany With Bioinformatics: New Insights in Genome Structure, DNA Methylation and Comparative Phylogenetic Analysis
title_full_unstemmed Revisiting Francisella tularensis subsp. holarctica, Causative Agent of Tularemia in Germany With Bioinformatics: New Insights in Genome Structure, DNA Methylation and Comparative Phylogenetic Analysis
title_short Revisiting Francisella tularensis subsp. holarctica, Causative Agent of Tularemia in Germany With Bioinformatics: New Insights in Genome Structure, DNA Methylation and Comparative Phylogenetic Analysis
title_sort revisiting francisella tularensis subsp. holarctica, causative agent of tularemia in germany with bioinformatics: new insights in genome structure, dna methylation and comparative phylogenetic analysis
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5859110/
https://www.ncbi.nlm.nih.gov/pubmed/29593661
http://dx.doi.org/10.3389/fmicb.2018.00344
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