Analysis of iodinated quorum sensing peptides by LC–UV/ESI ion trap mass spectrometry

Five different quorum sensing peptides (QSP) were iodinated using different iodination techniques. These iodinated peptides were analyzed using a C(18) reversed phase HPLC system, applying a linear gradient of water and acetonitrile containing 0.1% (m/v) formic acid as mobile phase. Electrospray ion...

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Detalles Bibliográficos
Autores principales: Janssens, Yorick, Verbeke, Frederick, Debunne, Nathan, Wynendaele, Evelien, Peremans, Kathelijne, De Spiegeleer, Bart
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Xi'an Jiaotong University 2018
Materias:
Short Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5859190/
https://www.ncbi.nlm.nih.gov/pubmed/29568670
http://dx.doi.org/10.1016/j.jpha.2017.09.001
Descripción
Sumario:Five different quorum sensing peptides (QSP) were iodinated using different iodination techniques. These iodinated peptides were analyzed using a C(18) reversed phase HPLC system, applying a linear gradient of water and acetonitrile containing 0.1% (m/v) formic acid as mobile phase. Electrospray ionization (ESI) ion trap mass spectrometry was used for the identification of the modified peptides, while semi-quantification was performed using total ion current (TIC) spectra. Non-iodinated peptides and mono- and di-iodinated peptides (NIP, MIP and DIP respectively) were well separated and eluted in that order. Depending on the used iodination method, iodination yields varied from low (2%) to high (57%).

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