Analysis of iodinated quorum sensing peptides by LC–UV/ESI ion trap mass spectrometry

Five different quorum sensing peptides (QSP) were iodinated using different iodination techniques. These iodinated peptides were analyzed using a C(18) reversed phase HPLC system, applying a linear gradient of water and acetonitrile containing 0.1% (m/v) formic acid as mobile phase. Electrospray ion...

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Autores principales: Janssens, Yorick, Verbeke, Frederick, Debunne, Nathan, Wynendaele, Evelien, Peremans, Kathelijne, De Spiegeleer, Bart
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Xi'an Jiaotong University 2018
Materias:
Short Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5859190/
https://www.ncbi.nlm.nih.gov/pubmed/29568670
http://dx.doi.org/10.1016/j.jpha.2017.09.001
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author Janssens, Yorick
Verbeke, Frederick
Debunne, Nathan
Wynendaele, Evelien
Peremans, Kathelijne
De Spiegeleer, Bart
author_facet Janssens, Yorick
Verbeke, Frederick
Debunne, Nathan
Wynendaele, Evelien
Peremans, Kathelijne
De Spiegeleer, Bart
author_sort Janssens, Yorick
collection PubMed
description Five different quorum sensing peptides (QSP) were iodinated using different iodination techniques. These iodinated peptides were analyzed using a C(18) reversed phase HPLC system, applying a linear gradient of water and acetonitrile containing 0.1% (m/v) formic acid as mobile phase. Electrospray ionization (ESI) ion trap mass spectrometry was used for the identification of the modified peptides, while semi-quantification was performed using total ion current (TIC) spectra. Non-iodinated peptides and mono- and di-iodinated peptides (NIP, MIP and DIP respectively) were well separated and eluted in that order. Depending on the used iodination method, iodination yields varied from low (2%) to high (57%).
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spelling pubmed-58591902018-03-22 Analysis of iodinated quorum sensing peptides by LC–UV/ESI ion trap mass spectrometry Janssens, Yorick Verbeke, Frederick Debunne, Nathan Wynendaele, Evelien Peremans, Kathelijne De Spiegeleer, Bart J Pharm Anal Short Communication Five different quorum sensing peptides (QSP) were iodinated using different iodination techniques. These iodinated peptides were analyzed using a C(18) reversed phase HPLC system, applying a linear gradient of water and acetonitrile containing 0.1% (m/v) formic acid as mobile phase. Electrospray ionization (ESI) ion trap mass spectrometry was used for the identification of the modified peptides, while semi-quantification was performed using total ion current (TIC) spectra. Non-iodinated peptides and mono- and di-iodinated peptides (NIP, MIP and DIP respectively) were well separated and eluted in that order. Depending on the used iodination method, iodination yields varied from low (2%) to high (57%). Xi'an Jiaotong University 2018-02 2017-09-14 /pmc/articles/PMC5859190/ /pubmed/29568670 http://dx.doi.org/10.1016/j.jpha.2017.09.001 Text en © 2018 Xi'an Jiaotong University. Production and hosting by Elsevier B.V. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Short Communication
Janssens, Yorick
Verbeke, Frederick
Debunne, Nathan
Wynendaele, Evelien
Peremans, Kathelijne
De Spiegeleer, Bart
Analysis of iodinated quorum sensing peptides by LC–UV/ESI ion trap mass spectrometry
title Analysis of iodinated quorum sensing peptides by LC–UV/ESI ion trap mass spectrometry
title_full Analysis of iodinated quorum sensing peptides by LC–UV/ESI ion trap mass spectrometry
title_fullStr Analysis of iodinated quorum sensing peptides by LC–UV/ESI ion trap mass spectrometry
title_full_unstemmed Analysis of iodinated quorum sensing peptides by LC–UV/ESI ion trap mass spectrometry
title_short Analysis of iodinated quorum sensing peptides by LC–UV/ESI ion trap mass spectrometry
title_sort analysis of iodinated quorum sensing peptides by lc–uv/esi ion trap mass spectrometry
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5859190/
https://www.ncbi.nlm.nih.gov/pubmed/29568670
http://dx.doi.org/10.1016/j.jpha.2017.09.001
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