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The mining and evolutionary investigation of AP2/ERF genes in pear (Pyrus)
BACKGROUND: In plants, ERF genes participate in a variety of regulatory pathways, such as plant growth and biotic and/or abiotic stress responses. Although the genome of Chinese white pear (‘Dangshansuli’) has been released, knowledge regarding the ERF family in pear, such as gene functions, evoluti...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5859658/ https://www.ncbi.nlm.nih.gov/pubmed/29558898 http://dx.doi.org/10.1186/s12870-018-1265-x |
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author | Li, Xiaolong Tao, Shutian Wei, Shuwei Ming, Meiling Huang, Xiaosan Zhang, Shaoling Wu, Jun |
author_facet | Li, Xiaolong Tao, Shutian Wei, Shuwei Ming, Meiling Huang, Xiaosan Zhang, Shaoling Wu, Jun |
author_sort | Li, Xiaolong |
collection | PubMed |
description | BACKGROUND: In plants, ERF genes participate in a variety of regulatory pathways, such as plant growth and biotic and/or abiotic stress responses. Although the genome of Chinese white pear (‘Dangshansuli’) has been released, knowledge regarding the ERF family in pear, such as gene functions, evolutionary history and expression patterns, remains limited. RESULTS: In our study, a total of 155 members of ERF families were identified in pear (Pyrus bretschneideri). The Ka and Ks values suggested that whole-genome duplication (WGD) and dispersed duplication have effectively contributed to the expansion of the pear ERF family. Gene structure and phylogeny analysis divided the PbrERF family into 12 groups, and their gene functions were predicted by comparative analysis. qRT-PCR was carried out to verify the relative expression levels of 7 genes in group III using wild and cultivated pear fruits at three key developmental stages. Wild samples had higher expression of these genes than cultivated samples, especially at the enlarged fruit stage. The transcriptome data of pear seedlings subjected to dehydration treatment further revealed that 4 of the 7 genes responded to drought conditions. CONCLUSION: The AP2/ERF gene family is greatly expanded in pear. Comparative analysis revealed the probability of ERF genes performing functional roles in multiple pathways. Expression analysis at different stages of pear fruit development in wild and cultivated samples indicated that genes in group III might be involved in abiotic and/or biotic stresses. Further transcriptome data on seedlings subjected to drought treatment verified the potential role of ERF genes in stress response. These results will provide a valuable reference for understanding the function and evolution of the ERF family in higher plants. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12870-018-1265-x) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-5859658 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-58596582018-03-22 The mining and evolutionary investigation of AP2/ERF genes in pear (Pyrus) Li, Xiaolong Tao, Shutian Wei, Shuwei Ming, Meiling Huang, Xiaosan Zhang, Shaoling Wu, Jun BMC Plant Biol Research Article BACKGROUND: In plants, ERF genes participate in a variety of regulatory pathways, such as plant growth and biotic and/or abiotic stress responses. Although the genome of Chinese white pear (‘Dangshansuli’) has been released, knowledge regarding the ERF family in pear, such as gene functions, evolutionary history and expression patterns, remains limited. RESULTS: In our study, a total of 155 members of ERF families were identified in pear (Pyrus bretschneideri). The Ka and Ks values suggested that whole-genome duplication (WGD) and dispersed duplication have effectively contributed to the expansion of the pear ERF family. Gene structure and phylogeny analysis divided the PbrERF family into 12 groups, and their gene functions were predicted by comparative analysis. qRT-PCR was carried out to verify the relative expression levels of 7 genes in group III using wild and cultivated pear fruits at three key developmental stages. Wild samples had higher expression of these genes than cultivated samples, especially at the enlarged fruit stage. The transcriptome data of pear seedlings subjected to dehydration treatment further revealed that 4 of the 7 genes responded to drought conditions. CONCLUSION: The AP2/ERF gene family is greatly expanded in pear. Comparative analysis revealed the probability of ERF genes performing functional roles in multiple pathways. Expression analysis at different stages of pear fruit development in wild and cultivated samples indicated that genes in group III might be involved in abiotic and/or biotic stresses. Further transcriptome data on seedlings subjected to drought treatment verified the potential role of ERF genes in stress response. These results will provide a valuable reference for understanding the function and evolution of the ERF family in higher plants. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12870-018-1265-x) contains supplementary material, which is available to authorized users. BioMed Central 2018-03-20 /pmc/articles/PMC5859658/ /pubmed/29558898 http://dx.doi.org/10.1186/s12870-018-1265-x Text en © The Author(s). 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Article Li, Xiaolong Tao, Shutian Wei, Shuwei Ming, Meiling Huang, Xiaosan Zhang, Shaoling Wu, Jun The mining and evolutionary investigation of AP2/ERF genes in pear (Pyrus) |
title | The mining and evolutionary investigation of AP2/ERF genes in pear (Pyrus) |
title_full | The mining and evolutionary investigation of AP2/ERF genes in pear (Pyrus) |
title_fullStr | The mining and evolutionary investigation of AP2/ERF genes in pear (Pyrus) |
title_full_unstemmed | The mining and evolutionary investigation of AP2/ERF genes in pear (Pyrus) |
title_short | The mining and evolutionary investigation of AP2/ERF genes in pear (Pyrus) |
title_sort | mining and evolutionary investigation of ap2/erf genes in pear (pyrus) |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5859658/ https://www.ncbi.nlm.nih.gov/pubmed/29558898 http://dx.doi.org/10.1186/s12870-018-1265-x |
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