Cargando…

The mining and evolutionary investigation of AP2/ERF genes in pear (Pyrus)

BACKGROUND: In plants, ERF genes participate in a variety of regulatory pathways, such as plant growth and biotic and/or abiotic stress responses. Although the genome of Chinese white pear (‘Dangshansuli’) has been released, knowledge regarding the ERF family in pear, such as gene functions, evoluti...

Descripción completa

Detalles Bibliográficos
Autores principales: Li, Xiaolong, Tao, Shutian, Wei, Shuwei, Ming, Meiling, Huang, Xiaosan, Zhang, Shaoling, Wu, Jun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5859658/
https://www.ncbi.nlm.nih.gov/pubmed/29558898
http://dx.doi.org/10.1186/s12870-018-1265-x
_version_ 1783307866814283776
author Li, Xiaolong
Tao, Shutian
Wei, Shuwei
Ming, Meiling
Huang, Xiaosan
Zhang, Shaoling
Wu, Jun
author_facet Li, Xiaolong
Tao, Shutian
Wei, Shuwei
Ming, Meiling
Huang, Xiaosan
Zhang, Shaoling
Wu, Jun
author_sort Li, Xiaolong
collection PubMed
description BACKGROUND: In plants, ERF genes participate in a variety of regulatory pathways, such as plant growth and biotic and/or abiotic stress responses. Although the genome of Chinese white pear (‘Dangshansuli’) has been released, knowledge regarding the ERF family in pear, such as gene functions, evolutionary history and expression patterns, remains limited. RESULTS: In our study, a total of 155 members of ERF families were identified in pear (Pyrus bretschneideri). The Ka and Ks values suggested that whole-genome duplication (WGD) and dispersed duplication have effectively contributed to the expansion of the pear ERF family. Gene structure and phylogeny analysis divided the PbrERF family into 12 groups, and their gene functions were predicted by comparative analysis. qRT-PCR was carried out to verify the relative expression levels of 7 genes in group III using wild and cultivated pear fruits at three key developmental stages. Wild samples had higher expression of these genes than cultivated samples, especially at the enlarged fruit stage. The transcriptome data of pear seedlings subjected to dehydration treatment further revealed that 4 of the 7 genes responded to drought conditions. CONCLUSION: The AP2/ERF gene family is greatly expanded in pear. Comparative analysis revealed the probability of ERF genes performing functional roles in multiple pathways. Expression analysis at different stages of pear fruit development in wild and cultivated samples indicated that genes in group III might be involved in abiotic and/or biotic stresses. Further transcriptome data on seedlings subjected to drought treatment verified the potential role of ERF genes in stress response. These results will provide a valuable reference for understanding the function and evolution of the ERF family in higher plants. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12870-018-1265-x) contains supplementary material, which is available to authorized users.
format Online
Article
Text
id pubmed-5859658
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-58596582018-03-22 The mining and evolutionary investigation of AP2/ERF genes in pear (Pyrus) Li, Xiaolong Tao, Shutian Wei, Shuwei Ming, Meiling Huang, Xiaosan Zhang, Shaoling Wu, Jun BMC Plant Biol Research Article BACKGROUND: In plants, ERF genes participate in a variety of regulatory pathways, such as plant growth and biotic and/or abiotic stress responses. Although the genome of Chinese white pear (‘Dangshansuli’) has been released, knowledge regarding the ERF family in pear, such as gene functions, evolutionary history and expression patterns, remains limited. RESULTS: In our study, a total of 155 members of ERF families were identified in pear (Pyrus bretschneideri). The Ka and Ks values suggested that whole-genome duplication (WGD) and dispersed duplication have effectively contributed to the expansion of the pear ERF family. Gene structure and phylogeny analysis divided the PbrERF family into 12 groups, and their gene functions were predicted by comparative analysis. qRT-PCR was carried out to verify the relative expression levels of 7 genes in group III using wild and cultivated pear fruits at three key developmental stages. Wild samples had higher expression of these genes than cultivated samples, especially at the enlarged fruit stage. The transcriptome data of pear seedlings subjected to dehydration treatment further revealed that 4 of the 7 genes responded to drought conditions. CONCLUSION: The AP2/ERF gene family is greatly expanded in pear. Comparative analysis revealed the probability of ERF genes performing functional roles in multiple pathways. Expression analysis at different stages of pear fruit development in wild and cultivated samples indicated that genes in group III might be involved in abiotic and/or biotic stresses. Further transcriptome data on seedlings subjected to drought treatment verified the potential role of ERF genes in stress response. These results will provide a valuable reference for understanding the function and evolution of the ERF family in higher plants. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12870-018-1265-x) contains supplementary material, which is available to authorized users. BioMed Central 2018-03-20 /pmc/articles/PMC5859658/ /pubmed/29558898 http://dx.doi.org/10.1186/s12870-018-1265-x Text en © The Author(s). 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Li, Xiaolong
Tao, Shutian
Wei, Shuwei
Ming, Meiling
Huang, Xiaosan
Zhang, Shaoling
Wu, Jun
The mining and evolutionary investigation of AP2/ERF genes in pear (Pyrus)
title The mining and evolutionary investigation of AP2/ERF genes in pear (Pyrus)
title_full The mining and evolutionary investigation of AP2/ERF genes in pear (Pyrus)
title_fullStr The mining and evolutionary investigation of AP2/ERF genes in pear (Pyrus)
title_full_unstemmed The mining and evolutionary investigation of AP2/ERF genes in pear (Pyrus)
title_short The mining and evolutionary investigation of AP2/ERF genes in pear (Pyrus)
title_sort mining and evolutionary investigation of ap2/erf genes in pear (pyrus)
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5859658/
https://www.ncbi.nlm.nih.gov/pubmed/29558898
http://dx.doi.org/10.1186/s12870-018-1265-x
work_keys_str_mv AT lixiaolong theminingandevolutionaryinvestigationofap2erfgenesinpearpyrus
AT taoshutian theminingandevolutionaryinvestigationofap2erfgenesinpearpyrus
AT weishuwei theminingandevolutionaryinvestigationofap2erfgenesinpearpyrus
AT mingmeiling theminingandevolutionaryinvestigationofap2erfgenesinpearpyrus
AT huangxiaosan theminingandevolutionaryinvestigationofap2erfgenesinpearpyrus
AT zhangshaoling theminingandevolutionaryinvestigationofap2erfgenesinpearpyrus
AT wujun theminingandevolutionaryinvestigationofap2erfgenesinpearpyrus
AT lixiaolong miningandevolutionaryinvestigationofap2erfgenesinpearpyrus
AT taoshutian miningandevolutionaryinvestigationofap2erfgenesinpearpyrus
AT weishuwei miningandevolutionaryinvestigationofap2erfgenesinpearpyrus
AT mingmeiling miningandevolutionaryinvestigationofap2erfgenesinpearpyrus
AT huangxiaosan miningandevolutionaryinvestigationofap2erfgenesinpearpyrus
AT zhangshaoling miningandevolutionaryinvestigationofap2erfgenesinpearpyrus
AT wujun miningandevolutionaryinvestigationofap2erfgenesinpearpyrus