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Baicalein Accelerates Tendon-Bone Healing via Activation of Wnt/β-Catenin Signaling Pathway in Rats

BACKGROUND: Tendon-bone healing is a reconstructive procedure which requires a tendon graft healing to a bone tunnel or to the surface of bone after the junction injury between tendon, ligament, and bone. The surgical reattachment of tendon to bone often fails due to regeneration failure of the spec...

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Autores principales: Tian, Xinggui, Jiang, Huaji, Chen, Yuhui, Ao, Xiang, Chen, Chuan, Zhang, Wentao, He, Feilin, Liao, Xiaoqing, Jiang, Xiaocheng, Li, Tao, Zhang, Zhongmin, Zhang, Xintao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5859801/
https://www.ncbi.nlm.nih.gov/pubmed/29693006
http://dx.doi.org/10.1155/2018/3849760
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author Tian, Xinggui
Jiang, Huaji
Chen, Yuhui
Ao, Xiang
Chen, Chuan
Zhang, Wentao
He, Feilin
Liao, Xiaoqing
Jiang, Xiaocheng
Li, Tao
Zhang, Zhongmin
Zhang, Xintao
author_facet Tian, Xinggui
Jiang, Huaji
Chen, Yuhui
Ao, Xiang
Chen, Chuan
Zhang, Wentao
He, Feilin
Liao, Xiaoqing
Jiang, Xiaocheng
Li, Tao
Zhang, Zhongmin
Zhang, Xintao
author_sort Tian, Xinggui
collection PubMed
description BACKGROUND: Tendon-bone healing is a reconstructive procedure which requires a tendon graft healing to a bone tunnel or to the surface of bone after the junction injury between tendon, ligament, and bone. The surgical reattachment of tendon to bone often fails due to regeneration failure of the specialized tendon-bone junction. MATERIALS AND METHODS: An extra-articular tendon-bone healing rat model was established to discuss the effect of the baicalein 10 mg/(kg·d) in accelerating tendon-bone healing progress. Also, tendon-derived stem cells (TDSCs) were treated with various concentrations of baicalein or dickkopf-1 (DKK-1) to stimulate differentiation for 14 days. RESULTS: In vivo, tendon-bone healing strength of experiment group was obviously stronger than the control group in 3 weeks as well as in 6 weeks. And there were more mature fibroblasts, more Sharpey fibers, and larger new bone formation area treated intragastrically with baicalein compared with rats that were treated with vehicle for 3 weeks and 6 weeks. In vitro, after induction for 14 days, the expressions of osteoblast differentiation markers, that is, alkaline phosphatase (ALP), runt-related transcription factor 2 (Runx2), osteocalcin (OCN), osterix (OSX), and collagen I, were upregulated and Wnt/β-catenin signaling pathway was enhanced in TDSCs. The effect of DKK-1 significantly reduced the effect of baicalein on the osteogenic differentiation. CONCLUSION: These data suggest that baicalein may stimulate TDSCs osteogenic differentiation via activation of Wnt/β-catenin signaling pathway to accelerate tendon-bone healing.
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spelling pubmed-58598012018-04-24 Baicalein Accelerates Tendon-Bone Healing via Activation of Wnt/β-Catenin Signaling Pathway in Rats Tian, Xinggui Jiang, Huaji Chen, Yuhui Ao, Xiang Chen, Chuan Zhang, Wentao He, Feilin Liao, Xiaoqing Jiang, Xiaocheng Li, Tao Zhang, Zhongmin Zhang, Xintao Biomed Res Int Research Article BACKGROUND: Tendon-bone healing is a reconstructive procedure which requires a tendon graft healing to a bone tunnel or to the surface of bone after the junction injury between tendon, ligament, and bone. The surgical reattachment of tendon to bone often fails due to regeneration failure of the specialized tendon-bone junction. MATERIALS AND METHODS: An extra-articular tendon-bone healing rat model was established to discuss the effect of the baicalein 10 mg/(kg·d) in accelerating tendon-bone healing progress. Also, tendon-derived stem cells (TDSCs) were treated with various concentrations of baicalein or dickkopf-1 (DKK-1) to stimulate differentiation for 14 days. RESULTS: In vivo, tendon-bone healing strength of experiment group was obviously stronger than the control group in 3 weeks as well as in 6 weeks. And there were more mature fibroblasts, more Sharpey fibers, and larger new bone formation area treated intragastrically with baicalein compared with rats that were treated with vehicle for 3 weeks and 6 weeks. In vitro, after induction for 14 days, the expressions of osteoblast differentiation markers, that is, alkaline phosphatase (ALP), runt-related transcription factor 2 (Runx2), osteocalcin (OCN), osterix (OSX), and collagen I, were upregulated and Wnt/β-catenin signaling pathway was enhanced in TDSCs. The effect of DKK-1 significantly reduced the effect of baicalein on the osteogenic differentiation. CONCLUSION: These data suggest that baicalein may stimulate TDSCs osteogenic differentiation via activation of Wnt/β-catenin signaling pathway to accelerate tendon-bone healing. Hindawi 2018-03-06 /pmc/articles/PMC5859801/ /pubmed/29693006 http://dx.doi.org/10.1155/2018/3849760 Text en Copyright © 2018 Xinggui Tian et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Tian, Xinggui
Jiang, Huaji
Chen, Yuhui
Ao, Xiang
Chen, Chuan
Zhang, Wentao
He, Feilin
Liao, Xiaoqing
Jiang, Xiaocheng
Li, Tao
Zhang, Zhongmin
Zhang, Xintao
Baicalein Accelerates Tendon-Bone Healing via Activation of Wnt/β-Catenin Signaling Pathway in Rats
title Baicalein Accelerates Tendon-Bone Healing via Activation of Wnt/β-Catenin Signaling Pathway in Rats
title_full Baicalein Accelerates Tendon-Bone Healing via Activation of Wnt/β-Catenin Signaling Pathway in Rats
title_fullStr Baicalein Accelerates Tendon-Bone Healing via Activation of Wnt/β-Catenin Signaling Pathway in Rats
title_full_unstemmed Baicalein Accelerates Tendon-Bone Healing via Activation of Wnt/β-Catenin Signaling Pathway in Rats
title_short Baicalein Accelerates Tendon-Bone Healing via Activation of Wnt/β-Catenin Signaling Pathway in Rats
title_sort baicalein accelerates tendon-bone healing via activation of wnt/β-catenin signaling pathway in rats
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5859801/
https://www.ncbi.nlm.nih.gov/pubmed/29693006
http://dx.doi.org/10.1155/2018/3849760
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