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Identification of small ORF-encoded peptides in mouse serum
Identification of the coding elements in the genome is fundamental to interpret the development of living systems and species diversity. Small peptides (length < 100 amino acids) have played an important role in regulating the biological metabolism, but their identification has been limited by th...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Berlin Heidelberg
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5860097/ https://www.ncbi.nlm.nih.gov/pubmed/29577068 http://dx.doi.org/10.1007/s41048-018-0048-0 |
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author | Deng, Yaqin Bamigbade, Adekunle Toyin Hammad, Mirza Ahmed Xu, Shimeng Liu, Pingsheng |
author_facet | Deng, Yaqin Bamigbade, Adekunle Toyin Hammad, Mirza Ahmed Xu, Shimeng Liu, Pingsheng |
author_sort | Deng, Yaqin |
collection | PubMed |
description | Identification of the coding elements in the genome is fundamental to interpret the development of living systems and species diversity. Small peptides (length < 100 amino acids) have played an important role in regulating the biological metabolism, but their identification has been limited by their size and abundance. Serum is the most important body fluid and is full of small peptides. In this study, we have established a small ORF-encoded peptides (SEPs) database from mouse GENCODE release. This database provides about half a million putative translated SEPs in mouse. We also extract serum proteins from wild type and ob/ob mice, and collect the low molecular weight proteins for mass spectrometric analysis. More than 50 novel SEPs have been discovered. Several SEPs are further verified by biochemical method with newly raised antibodies. These novel SEPs enhance the knowledge about the complexity of serum and provide new clues for the annotation and functional analysis of genes, especially the noncoding elements in the genome. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s41048-018-0048-0) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-5860097 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Springer Berlin Heidelberg |
record_format | MEDLINE/PubMed |
spelling | pubmed-58600972018-03-22 Identification of small ORF-encoded peptides in mouse serum Deng, Yaqin Bamigbade, Adekunle Toyin Hammad, Mirza Ahmed Xu, Shimeng Liu, Pingsheng Biophys Rep Research Article Identification of the coding elements in the genome is fundamental to interpret the development of living systems and species diversity. Small peptides (length < 100 amino acids) have played an important role in regulating the biological metabolism, but their identification has been limited by their size and abundance. Serum is the most important body fluid and is full of small peptides. In this study, we have established a small ORF-encoded peptides (SEPs) database from mouse GENCODE release. This database provides about half a million putative translated SEPs in mouse. We also extract serum proteins from wild type and ob/ob mice, and collect the low molecular weight proteins for mass spectrometric analysis. More than 50 novel SEPs have been discovered. Several SEPs are further verified by biochemical method with newly raised antibodies. These novel SEPs enhance the knowledge about the complexity of serum and provide new clues for the annotation and functional analysis of genes, especially the noncoding elements in the genome. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s41048-018-0048-0) contains supplementary material, which is available to authorized users. Springer Berlin Heidelberg 2018-03-08 2018 /pmc/articles/PMC5860097/ /pubmed/29577068 http://dx.doi.org/10.1007/s41048-018-0048-0 Text en © The Author(s) 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. |
spellingShingle | Research Article Deng, Yaqin Bamigbade, Adekunle Toyin Hammad, Mirza Ahmed Xu, Shimeng Liu, Pingsheng Identification of small ORF-encoded peptides in mouse serum |
title | Identification of small ORF-encoded peptides in mouse serum |
title_full | Identification of small ORF-encoded peptides in mouse serum |
title_fullStr | Identification of small ORF-encoded peptides in mouse serum |
title_full_unstemmed | Identification of small ORF-encoded peptides in mouse serum |
title_short | Identification of small ORF-encoded peptides in mouse serum |
title_sort | identification of small orf-encoded peptides in mouse serum |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5860097/ https://www.ncbi.nlm.nih.gov/pubmed/29577068 http://dx.doi.org/10.1007/s41048-018-0048-0 |
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