uvCLAP is a fast and non-radioactive method to identify in vivo targets of RNA-binding proteins

RNA-binding proteins (RBPs) play important and essential roles in eukaryotic gene expression regulating splicing, localization, translation, and stability of mRNAs. We describe ultraviolet crosslinking and affinity purification (uvCLAP), an easy-to-use, robust, reproducible, and high-throughput meth...

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Detalles Bibliográficos
Autores principales: Maticzka, Daniel, Ilik, Ibrahim Avsar, Aktas, Tugce, Backofen, Rolf, Akhtar, Asifa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5861125/
https://www.ncbi.nlm.nih.gov/pubmed/29559621
http://dx.doi.org/10.1038/s41467-018-03575-4

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5861125/
https://www.ncbi.nlm.nih.gov/pubmed/29559621
http://dx.doi.org/10.1038/s41467-018-03575-4

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