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uvCLAP is a fast and non-radioactive method to identify in vivo targets of RNA-binding proteins
RNA-binding proteins (RBPs) play important and essential roles in eukaryotic gene expression regulating splicing, localization, translation, and stability of mRNAs. We describe ultraviolet crosslinking and affinity purification (uvCLAP), an easy-to-use, robust, reproducible, and high-throughput meth...
| Autores principales: | , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Nature Publishing Group UK
2018
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5861125/ https://www.ncbi.nlm.nih.gov/pubmed/29559621 http://dx.doi.org/10.1038/s41467-018-03575-4 |