Expression of recombinant HBD3 protein that reduces Mycobacterial infection capacity

Bovine tuberculosis is a disease caused by Mycobacterium bovis (M. bovis) that leads to great economic losses in cattle production. The discovery of a reasonable bioagent to reduce M. bovis infection risk and environment contamination becomes significant and urgent. Previous study reported that huma...

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Detalles Bibliográficos
Autores principales: Su, Feng, Chen, Xin, Liu, Xin, Liu, Guanghui, Zhang, Yong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2018
Materias:
Original Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5861256/
https://www.ncbi.nlm.nih.gov/pubmed/29556853
http://dx.doi.org/10.1186/s13568-018-0573-8
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author Su, Feng
Chen, Xin
Liu, Xin
Liu, Guanghui
Zhang, Yong
author_facet Su, Feng
Chen, Xin
Liu, Xin
Liu, Guanghui
Zhang, Yong
author_sort Su, Feng
collection PubMed
description Bovine tuberculosis is a disease caused by Mycobacterium bovis (M. bovis) that leads to great economic losses in cattle production. The discovery of a reasonable bioagent to reduce M. bovis infection risk and environment contamination becomes significant and urgent. Previous study reported that human β-defensin-3 (HBD3) participated in Mycobacterial immunity and was recognized as a suitable candidate reagent. However, its minimal inhibitory concentration to M. bovis is not yet reported. In this study, we first purified HBD3 protein by recombinant-DNA technology and prokaryotic expression system. Subsequently, anti-bacterial tests were used to evaluate the basic bioactivity of the protein. Results revealed that recombinant HBD3 (rHBD3) protein inhibits Staphylococcus multiplication but not the host Escherichia coli. The growth curve of M. bovis showed that rHBD3 protein controls the proliferation of M. bovis in 20 μg/ml concentration. In addition, rHBD3 protein-incubated M. bovis exhibited reduced infectivity to alveolar epithelial cells and macrophages. In conclusion, the expression of rHBD3 protein is a potential ideal bio-regent for reducing M. bovis infection.
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spelling pubmed-58612562018-03-23 Expression of recombinant HBD3 protein that reduces Mycobacterial infection capacity Su, Feng Chen, Xin Liu, Xin Liu, Guanghui Zhang, Yong AMB Express Original Article Bovine tuberculosis is a disease caused by Mycobacterium bovis (M. bovis) that leads to great economic losses in cattle production. The discovery of a reasonable bioagent to reduce M. bovis infection risk and environment contamination becomes significant and urgent. Previous study reported that human β-defensin-3 (HBD3) participated in Mycobacterial immunity and was recognized as a suitable candidate reagent. However, its minimal inhibitory concentration to M. bovis is not yet reported. In this study, we first purified HBD3 protein by recombinant-DNA technology and prokaryotic expression system. Subsequently, anti-bacterial tests were used to evaluate the basic bioactivity of the protein. Results revealed that recombinant HBD3 (rHBD3) protein inhibits Staphylococcus multiplication but not the host Escherichia coli. The growth curve of M. bovis showed that rHBD3 protein controls the proliferation of M. bovis in 20 μg/ml concentration. In addition, rHBD3 protein-incubated M. bovis exhibited reduced infectivity to alveolar epithelial cells and macrophages. In conclusion, the expression of rHBD3 protein is a potential ideal bio-regent for reducing M. bovis infection. Springer Berlin Heidelberg 2018-03-20 /pmc/articles/PMC5861256/ /pubmed/29556853 http://dx.doi.org/10.1186/s13568-018-0573-8 Text en © The Author(s) 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
spellingShingle Original Article
Su, Feng
Chen, Xin
Liu, Xin
Liu, Guanghui
Zhang, Yong
Expression of recombinant HBD3 protein that reduces Mycobacterial infection capacity
title Expression of recombinant HBD3 protein that reduces Mycobacterial infection capacity
title_full Expression of recombinant HBD3 protein that reduces Mycobacterial infection capacity
title_fullStr Expression of recombinant HBD3 protein that reduces Mycobacterial infection capacity
title_full_unstemmed Expression of recombinant HBD3 protein that reduces Mycobacterial infection capacity
title_short Expression of recombinant HBD3 protein that reduces Mycobacterial infection capacity
title_sort expression of recombinant hbd3 protein that reduces mycobacterial infection capacity
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5861256/
https://www.ncbi.nlm.nih.gov/pubmed/29556853
http://dx.doi.org/10.1186/s13568-018-0573-8
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